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1. 发明申请

US20050277203A1 Immunochemical filter device and methods for use thereof 有权
标题翻译：免疫化学过滤装置及其使用方法
公开(公告)号：US20050277203A1
公开(公告)日：2005-12-15
申请号：US10954627
申请日：2004-09-29
申请人： Aimo Niskanen
发明人： Aimo Niskanen
IPC分类号： C12M1/26 , C12M1/34 , G01N20060101 , G01N33/53 , G01N33/543
CPC分类号： G01N33/5304 , Y10T436/25 , Y10T436/25375 , Y10T436/255
摘要： The invention provides an immunochemical filter device and use thereof, said filter device comprising a filter material attached to a support member. The filter material comprises a labeled binding reagent, wherein said labeled binding reagent is released from the filter material into solution by migration of a liquid sample solution through the filter material. The mixture of the sample solution and the labeled specific binding reagent is transferred to an analyzer device comprising a porous carrier, preferably by expressing the mixture through an aperture, diffusible membrane or valve in the support member. Additionally the invention provides a method for determining the presence or absence of an analyte in a sample solution and further provides a kit comprising the filter device.
摘要翻译：本发明提供一种免疫化学过滤装置及其用途，所述过滤装置包括附着于支撑构件的过滤材料。过滤材料包括标记的结合试剂，其中所述标记的结合试剂通过液体样品溶液通过过滤材料迁移而从过滤材料释放到溶液中。将样品溶液和标记的特异性结合试剂的混合物转移到包含多孔载体的分析装置中，优选通过将支持构件中的孔，扩散膜或阀表示混合物。另外，本发明提供了一种用于确定样品溶液中分析物的存在或不存在的方法，并且还提供了包括过滤装置的试剂盒。

2. 发明申请

US20050272601A1 Composition and devices for gas sorption and process for their manufacturing 有权
标题翻译：气体吸附的组成和装置及其制造过程
公开(公告)号：US20050272601A1
公开(公告)日：2005-12-08
申请号：US11199424
申请日：2005-08-08
申请人： Lorena Cattaneo , Alessandro Gallitognotta
发明人： Lorena Cattaneo , Alessandro Gallitognotta
IPC分类号： B01J20/26 , C08K3/00 , G01N20060101 , G01N1/00
CPC分类号： B01J20/28026 , B01J20/041 , B01J20/06 , B01J20/08 , B01J20/26 , B01J20/262 , B01J20/28004 , B01J20/3078
摘要： Compositions and devices for gas sorption are provided that can be activated just before their use, which hence do not need storing under inert atmosphere during storage and transportation.
摘要翻译：提供用于气体吸附的组合物和装置，其可以在其使用之前被激活，因此在储存和运输期间不需要在惰性气氛下储存。

3. 发明申请

US20050272105A1 Bacterial biosensors 审中-公开
标题翻译：细菌生物传感器
公开(公告)号：US20050272105A1
公开(公告)日：2005-12-08
申请号：US10888530
申请日：2004-07-09
申请人： Kalle Levon , Bin Yu , Yanxiu Zhou
发明人： Kalle Levon , Bin Yu , Yanxiu Zhou
IPC分类号： A61F13/15 , C12M1/34 , C12Q1/00 , G01N20060101 , G01N33/543 , G01N33/552 , G01N33/554 , G01N33/569
CPC分类号： C12Q1/00 , C07K9/00 , C07K14/32 , C07K17/00 , C12Q1/003 , G01N33/53 , G01N33/54353 , G01N33/5438 , G01N33/54393 , G01N33/552
摘要： A real-time, portable peptide-containing potentiometric biosensor that can directly identify bacterial spores. Two peptides for specific recognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example, B. subtilis spores in a concentration range of 0.08-7.3×104 CFU/ml. The sensor exhibited highly selective recognition properties towards Bacillus subtilis spores over other kinds of spores. The selectivity coefficients of the sensors for other kinds of spores are in the range of 0-1.0×10−5. The biosensor system not only has the specificity to distinguish Bacillus subtilis spores in a mixture of B. subtilis and B. thuringiensis (thur.) Kurstaki spores, but also can discriminate between live and dead B. subtilis spores. Furthermore, the sensor can distinguish a Bacillus subtilis 1A700 from other B. subtilis strain. Assay time may be as low as about 5 minutes for a single test. Rapid identification of B. anthracis Sterne and B. anthracis ΔAmes was also provided.
摘要翻译：一种可直接鉴定细菌孢子的实时便携式含肽电位生物传感器。用于特异性识别枯草芽孢杆菌和炭疽芽孢杆菌的两种肽可以通过聚硅氧烷单层固定（PMI）技术来固定。传感器通过检测例如浓度范围为0.08-7.3×10 4 CFU / ml的枯草芽孢杆菌孢子将生物识别事件转化为潜在的变化。传感器表现出对枯草芽孢杆菌孢子与其他种类孢子的高选择性识别性能。用于其他种类孢子的传感器的选择性系数在0-1.0×10 -5的范围内。生物传感器系统不仅具有将枯草芽孢杆菌和苏云金芽孢杆菌（thur。）Kurstaki孢子的混合物中的枯草芽孢杆菌孢子区分开的特异性，而且可以区分活枯枯病芽孢杆菌孢子和活枯草芽孢杆菌孢子。此外，传感器可以将枯草芽孢杆菌1A700与其他枯草芽孢杆菌菌株区分开来。单次测试的测定时间可能低至约5分钟。炭疽杆菌和炭疽杆菌的快速鉴定也提供了DeltaAmes。

4. 发明授权

US06969857B2 Compensated infrared absorption sensor for carbon dioxide and other infrared absorbing gases 有权
标题翻译：用于二氧化碳和其他红外吸收气体的补偿红外吸收传感器
公开(公告)号：US06969857B2
公开(公告)日：2005-11-29
申请号：US10753614
申请日：2004-01-08
申请人： Thomas E. Owen
发明人： Thomas E. Owen
IPC分类号： G01J3/427 , G01N20060101 , G01N21/05 , G01N21/31 , G01N21/35 , G01N21/61 , G01N33/00
CPC分类号： G01N21/3518 , G01J3/0232 , G01J3/427 , G01N21/05 , G01N21/314 , G01N21/3504 , G01N21/61 , G01N33/004 , G01N33/0054 , G01N2021/3125 , G01N2021/3137 , G01N2201/0675 , G01N2201/1211 , G01N2201/1218 , Y02A50/244 , Y02A50/246
摘要： A gas sensor, whose chamber uses filters and choppers in either a semicircular geometry or annular geometry, and incorporates separate infrared radiation filters and optical choppers. This configuration facilitates the use of a single infrared radiation source and a single detector for infrared measurements at two wavelengths, such that measurement errors may be compensated.
摘要翻译：气体传感器，其室使用半圆形几何形状或环形几何形状的过滤器和斩波器，并且包含单独的红外辐射滤波器和光学切割器。这种配置有助于在两个波长处使用单个红外辐射源和单个检测器用于红外测量，从而可以补偿测量误差。

5. 发明申请

US20050247871A1 Combined chemical/biological agent detection system and method utilizing mass spectrometry 有权
标题翻译：组合化学/生物试剂检测系统和利用质谱法的方法
公开(公告)号：US20050247871A1
公开(公告)日：2005-11-10
申请号：US10515300
申请日：2003-07-17
申请人： Wayne Bryden , Robert Cotter , Scott Ecelberger
发明人： Wayne Bryden , Robert Cotter , Scott Ecelberger
IPC分类号： B01D59/44 , G01N20060101 , H01J49/00 , H01J49/04 , H01J49/10 , H01J49/16 , H01J49/40
CPC分类号： H01J49/147 , H01J49/107
摘要： A mass spectrometer is provided herein and is configured to have two ionization sources, in which a first ionization source, such as MALDI, ESI and the like, which is capable of providing in addition to ions a set of normally intractable desorbed neutrals that are ionized by a second EI source coupled with the first source.
摘要翻译：本文提供了一种质谱仪，并且被配置为具有两个电离源，其中第一电离源如MALDI，ESI等能够提供除离子之外的一组正常难溶的解吸的中性粒子，其被离子化通过与第一源耦合的第二EI源。

6. 发明申请

US20050233470A1 Methods and compound mixtures for determining protein activity using nmr spectroscopy 审中-公开
标题翻译：使用nmr光谱法测定蛋白质活性的方法和化合物混合物
公开(公告)号：US20050233470A1
公开(公告)日：2005-10-20
申请号：US10511960
申请日：2003-04-15
申请人： Bill Clark , Klaes Golman , Mathilde Lerche , Mike Looker , Mike O'Sullivan , Albie Santos , Rolf Servin , Mikkel Thaning , Jan-Henrik Ardenkjaer-Larsen
发明人： Bill Clark , Klaes Golman , Mathilde Lerche , Mike Looker , Mike O'Sullivan , Albie Santos , Rolf Servin , Mikkel Thaning , Jan-Henrik Ardenkjaer-Larsen
IPC分类号： G01R33/465 , C12Q1/00 , C12Q1/26 , C12Q1/48 , G01N20060101 , G01N24/00 , G01N24/08 , G01R33/28
CPC分类号： G01N24/088 , G01R33/282 , G01R33/465 , Y10T436/24
摘要： The invention relates to methods for determining protein activity using NMR spectroscopy. The present invention provides a method for determining protein activity in vivo using probe compounds and enhancing the nuclear polarisation of NMR active nuclei present in the probe compounds (hereinafter termed “hyperpolarisation”) prior to NMR analysis. The invention also provides mixtures of probe compounds for the above-mentioned method.
摘要翻译：本发明涉及使用NMR光谱测定蛋白质活性的方法。本发明提供了在NMR分析之前使用探针化合物和增强存在于探针化合物中的NMR活性核（以下称为“超极化”）的核偏振来测定体内蛋白质活性的方法。本发明还提供了用于上述方法的探针化合物的混合物。

7. 发明申请

US20050221324A1 Genotoxicity analysis 审中-公开
标题翻译：遗传毒性分析
公开(公告)号：US20050221324A1
公开(公告)日：2005-10-06
申请号：US10513489
申请日：2003-05-06
申请人： Michael Fox , Charles Waldren
发明人： Michael Fox , Charles Waldren
IPC分类号： C12N5/20 , C12N15/85 , C12Q1/68 , G01N20060101 , G01N33/50 , G01N33/566 , G01N33/569
CPC分类号： G01N33/5014 , G01N33/566 , G01N33/56966 , G01N2333/70585 , G01N2333/70596
摘要： Genotoxicity testing can be carried out using a genetic hybrid cell line, for example, a CHO cell line which contains human chromosome 11. This exemplified hybrid cell line expresses human CD59 on the cell surface, and the human CD59 gene serves as a test for mutagenic agents. The hybrid cell line is grown in the presence of a test compound, and the loss of cell surface CD59 is followed using a fluorescent-labeled antibody specific for human CD59 and flow cytometry to monitor the presence or absence of labeled antibody on particular cells. Absence of the labeled antibody on the surface of the cells is indicative of a mutation in the CD59 gene such that either no CD59 protein is made or there has been a mutation which results in the loss of the antibody binding site. A test compound which causes CD59 loss is deemed to be genotoxic (i.e., mutagenic). The mutations can be point mutations, deletions, inversions, insertions, or frameshifts. The sensitivity of the assay is improved when the cells are first panned with antibody specific for the cell surface marker to remove spontaneous mutants prior to challenge with the test compound. Alternatively, or in addition, an antibiotic resistance marker is incorporated onto the same chromosome as the cell surface marker, and an antibiotic selection step precedes the challenge with the potential genotoxic composition or condition.
摘要翻译：可以使用遗传杂交细胞系，例如包含人染色体11的CHO细胞系进行基因毒性测试。这个例示的杂交细胞系在细胞表面表达人CD59，并且人CD59基因用作诱变的试验代理商杂交细胞系在受试化合物的存在下生长，并且使用针对人CD59特异性的荧光标记抗体和流式细胞术来监测细胞表面CD59的丧失，以监测特定细胞上标记抗体的存在或不存在。在细胞表面没有标记的抗体指示CD59基因中的突变，使得不产生CD59蛋白，或者存在导致抗体结合位点丧失的突变。造成CD59损失的试验化合物被认为是遗传毒性（即致突变性的）。突变可以是点突变，缺失，倒置，插入或移码。当细胞首先用针对细胞表面标志物的抗体特异性去除以在用测试化合物攻击之前去除自发突变体时，测定的灵敏度得到改善。或者或另外，将抗生素抗性标记并入细胞表面标志物的相同染色体上，并且抗生素选择步骤在潜在的遗传毒性组合物或病症的攻击之前进行。

8. 发明申请

US20050208580A1 Novel protein and method for producing the protein 审中-公开
标题翻译：新型蛋白质及其生产方法
公开(公告)号：US20050208580A1
公开(公告)日：2005-09-22
申请号：US11135521
申请日：2005-05-24
申请人： Kyoji Yamaguchi , Hisataka Yasuda , Nobuaki Nakagawa , Nobuyuki Shima , Masahiko Kinosaki , Eisuke Tsuda , Masaaki Goto , Kazuki Yano , Akihiro Tomoyasu , Fumie Kobayashi , Naohiro Washida , Ken Takahashi , Tomonori Morinaga , Kanji Higashio
发明人： Kyoji Yamaguchi , Hisataka Yasuda , Nobuaki Nakagawa , Nobuyuki Shima , Masahiko Kinosaki , Eisuke Tsuda , Masaaki Goto , Kazuki Yano , Akihiro Tomoyasu , Fumie Kobayashi , Naohiro Washida , Ken Takahashi , Tomonori Morinaga , Kanji Higashio
IPC分类号： C07K14/715 , A61K20060101 , A61K38/00 , A61K39/395 , A61K45/00 , A61P3/14 , A61P19/00 , A61P19/02 , A61P19/08 , A61P19/10 , A61P29/00 , B01J20/281 , C07K20060101 , C07K1/22 , C07K14/47 , C07K14/525 , C07K14/705 , C07K16/18 , C07K16/28 , C12N20060101 , C12N5/10 , C12N5/20 , C12N15/02 , C12N15/09 , C12N15/12 , C12P20060101 , C12P21/02 , C12P21/08 , C12Q20060101 , G01N20060101 , G01N30/88 , G01N33/15 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/577 , C12Q1/68 , C07H21/04
CPC分类号： C07K16/2875 , A61K38/00 , A61K2039/505 , C07K14/525 , C07K14/705 , C07K14/70578 , C07K16/28 , C07K16/2878 , C07K2317/76 , C07K2317/92 , C07K2319/00 , G01N33/566 , G01N2333/525 , G01N2333/70578 , G01N2800/108
摘要： A novel protein which binds to Osteoclastogenesis Inhibitory Factor (OCIF-binding molecule; OBM), a process for preparing the same, DNA encoding said protein, a protein having an amino acid sequence encoded by this DNA, a method for producing said protein by genetic engineering technique, and a pharmaceutical composition containing said protein. Screening methods for a substance for controlling expression of said protein using said protein and the DNA, a substance which inhibits or modulates the biological activity of said protein, or a receptor which transmits the action of said protein through binding to said protein, the substance obtained by the screening methods, and a pharmaceutical composition which contains this substance. An antibody for said protein, a process for preparing the same, a measuring method of said protein using the antibody, and a medicine comprising this antibody.
摘要翻译：结合破骨细胞发生抑制因子（OCIF-结合分子; OBM）的新型蛋白质，其制备方法，编码所述蛋白质的DNA，由该DNA编码的氨基酸序列的蛋白质，通过遗传学生产所述蛋白质的方法工程技术和含有该蛋白质的药物组合物。使用所述蛋白质和DNA控制所述蛋白质的表达的物质的筛选方法，抑制或调节所述蛋白质的生物活性的物质，或通过与所述蛋白质结合而转移所述蛋白质的作用的受体，获得的物质通过筛选方法和含有该物质的药物组合物。所述蛋白质的抗体，其制备方法，使用该抗体的所述蛋白质的测定方法，以及包含该抗体的药物。

9. 发明申请

US20050202503A1 Displacement assay for detection of small molecules 有权
标题翻译：用于检测小分子的位移测定
公开(公告)号：US20050202503A1
公开(公告)日：2005-09-15
申请号：US11123648
申请日：2005-05-06
申请人： Milan Stojanovic , Donald Landry
发明人： Milan Stojanovic , Donald Landry
IPC分类号： A01N61/00 , C07H21/02 , C07H21/04 , C07K1/00 , C07K16/00 , C12M1/34 , C12Q1/00 , C12Q1/68 , C12Q1/70 , G01N20060101 , G01N33/53 , G01N33/566
CPC分类号： G01N33/566 , Y10T436/143333
摘要： Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a calorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2-5000 μM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of the sensor. As the dye is insoluble in buffer, cocaine binding can be detected as displaced dye precipitates and supernatant decolorizes.
摘要翻译：抗可卡因适配体和染料二乙基硫代花青的复合物作为量热传感器，其浓度范围为2-5000μM，可卡因在760nm处的吸光度衰减。机理研究表明染料的分子间位移作为传感器的作用机理。由于染料不溶于缓冲液，可检测到可卡因结合作为置换的染料沉淀物，上清液脱色。

10. 发明申请

US20050181452A1 Fragment complementation assays for G-protein-coupled receptors and their signaling pathways 失效
标题翻译： G蛋白偶联受体及其信号通路的片段互补测定
公开(公告)号：US20050181452A1
公开(公告)日：2005-08-18
申请号：US10947368
申请日：2004-09-23
申请人： John Westwick , Brigitte Keon , Marnie MacDonald
发明人： John Westwick , Brigitte Keon , Marnie MacDonald
IPC分类号： C07K16/28 , C12P21/02 , C12Q1/00 , C12Q1/68 , G01N20060101 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/74
CPC分类号： G01N33/74 , C12Q1/00 , G01N33/5041 , G01N2333/726 , G01N2500/00
摘要： This invention relates generally to the fields of biology, molecular biology, chemistry and biochemistry. The invention is directed to a large number of novel assays for G-protein-coupled receptors (GPCRs) and their signaling pathways. Methods are described for constructing such assays for one or more steps in a GPCR pathway. The invention can be used for functional characterization of GPCRs, target validation, de-orphanization of receptors, high-throughput screening, high-content screening, pharmacological profiling, and other drug discovery applications. The assays can be used directly to assess whether a compound library or a biological extract contains an agonist or antagonist of a receptor. Assay compositions are also provided. The development of such assays is shown to be straightforward, providing for a broad, flexible and biologically relevant platform for the discovery of novel drugs and natural ligands that act on GPCRs or their cognate pathways.
摘要翻译：本发明一般涉及生物学，分子生物学，化学和生物化学领域。本发明涉及大量用于G蛋白偶联受体（GPCR）的新型测定及其信号通路。描述了用于构建GPCR途径中一个或多个步骤的这种测定法的方法。本发明可用于GPCR的功能表征，目标验证，受体去孤儿化，高通量筛选，高含量筛选，药理学分析和其他药物发现应用。所述测定可直接用于评价化合物文库或生物提取物是否含有受体的激动剂或拮抗剂。还提供了测定组合物。显示出这种测定的发展是直接的，为广泛，灵活和生物学上相关的平台发现用于GPCR或其同源途径的新型药物和天然配体的发现。

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