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1. 发明授权

US4212946A Process for recovering protease 失效
标题翻译：回收蛋白酶的方法
公开(公告)号：US4212946A
公开(公告)日：1980-07-15
申请号：US907203
申请日：1978-05-18
申请人： Yuji Nonaka , Kiyotaka Oyama , Heijiro Satoh
发明人： Yuji Nonaka , Kiyotaka Oyama , Heijiro Satoh
IPC分类号： C12N9/00 , C07K5/06 , C07K5/075 , C12N9/50 , C12P21/02 , C12N9/48 , C07G7/02
CPC分类号： C12N9/50 , C12P21/02 , Y10S435/816
摘要： Protease is recovered by reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group, in an aqueous medium in the presence of a protease to result a peptide synthesis to deposit the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said addition compound by adding an organic solvent to a precipitate separated from the reaction mixture and isolating the protease from an organic solvent suspension or dissolving said addition compound by adding a water immiscible organic solvent and separating the organic solvent phase by a liquid-liquid separation.The addition compound can be dissolved in an organic solvent after separating the precipitate from the reaction mixture.The addition compound can be also dissolved by adding a water-immiscible organic solvent to the reaction mixture and the organic solvent phase can be separated from the aqueous phase.
摘要翻译：通过使氨基被保护基团的第一个氨基酸与其羧基被保护基团保护的第二个氨基酸在水性介质中在蛋白酶存在下反应，从而使肽合成沉积，从而回收蛋白酶二肽和所述第二C末端保护的氨基酸的加成化合物; 通过向从反应混合物分离的沉淀物中加入有机溶剂来溶解所述加成化合物，并从有机溶剂悬浮液中分离蛋白酶或通过加入与水不混溶的有机溶剂溶解所述加成化合物并通过液 - 液分离分离有机溶剂相。在从反应混合物中分离出沉淀物后，加成化合物可以溶解在有机溶剂中。加成化合物也可以通过向反应混合物中加入与水不混溶的有机溶剂而溶解，并且有机溶剂相可以与水相分离。

2. 发明授权

US4212945A Process for recovering protease 失效
标题翻译：回收蛋白酶的方法
公开(公告)号：US4212945A
公开(公告)日：1980-07-15
申请号：US907006
申请日：1978-05-18
申请人： Yuji Nonaka , Kiyotaka Oyama , Heijiro Satoh
发明人： Yuji Nonaka , Kiyotaka Oyama , Heijiro Satoh
IPC分类号： C12N9/00 , C07K5/06 , C07K5/075 , C12N9/50 , C12P21/02 , C12N9/48 , C07G7/02
CPC分类号： C12N9/50 , C12P21/02 , Y10S435/816
摘要： Protease is recovered by reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group, in an aqueous medium in the presence of a protease to result a peptide synthesis to deposit the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said addition compound into the aqueous medium by adding a polar organic solvent which is miscible with water and separating an insoluble material from the resulting suspension by a solid-liquid separation to isolate the protease.
摘要翻译：通过使氨基被保护基团的第一个氨基酸与其羧基被保护基团保护的第二个氨基酸在水性介质中在蛋白酶存在下反应，从而使肽合成沉积，从而回收蛋白酶二肽和所述第二C末端保护的氨基酸的加成化合物; 通过加入与水混溶的极性有机溶剂将所述加成化合物溶解在水性介质中，并通过固液分离从所得悬浮液中分离出不溶物，分离蛋白酶。

3. 发明授权

US4436925A Addition compound of dipeptide derivative and amino acid derivative 失效
标题翻译：二肽衍生物和氨基酸衍生物的加成化合物
公开(公告)号：US4436925A
公开(公告)日：1984-03-13
申请号：US270025
申请日：1982-06-03
申请人： Yoshikazu Isowa , Muneki Ohmori , Kaoru Mori , Tetsuya Ichikawa , Yuji Nonaka , Keiichi Kihara , Kiyotaka Oyama , Heijiro Satoh , Shigeaki Nishimura
发明人： Yoshikazu Isowa , Muneki Ohmori , Kaoru Mori , Tetsuya Ichikawa , Yuji Nonaka , Keiichi Kihara , Kiyotaka Oyama , Heijiro Satoh , Shigeaki Nishimura
IPC分类号： C07K5/072 , C07K5/075 , C07C101/00
CPC分类号： C07K5/0613
摘要： Addition compounds have the formula ##STR1## wherein R.sub.1 represents an aliphatic oxycarbonyl group, benzyloxycarbonyl group which can have nuclear substituents or benzoyl, aromatic sulfonyl or aromatic sulfinyl group; R.sub.2 represents methyl, isopropyl, isobutyl, isoamyl or benzyl group; R.sub.3 represents a lower alkoxyl, benzyloxy or benzhydryloxy group and n represents 1 or 2.The addition compounds are produced by reacting an N-substituted monoaminodicarboxylic acid having the formula ##STR2## with an amino carboxlic acid ester having the formula in an aqueous medium in the presence of a protease and reacting the resulting dipeptide ester with the amino carboxylic acid ester and separating the addition compound.The addition compounds can be decomposed into the constituent dipeptide esters and amino acid esters by the action of an acid.
摘要翻译：加成化合物具有式（I）其中R 1表示脂族氧羰基，可具有核取代基的苄氧基羰基或苯甲酰基，芳族磺酰基或芳族亚磺酰基; R 2表示甲基，异丙基，异丁基，异戊基或苄基; R3表示低级烷氧基，苄氧基或二苯甲氧基，n表示1或2.加成化合物通过使具有式（IV）的式（I）所示的N-取代的单氨基二羧酸与式（V）在水性介质中，在蛋白酶存在下，使得到的二肽酯与氨基羧酸酯反应并分离加成化合物。加成化合物可以通过酸的作用分解成组分二肽酯和氨基酸酯。

4. 发明授权

US4256836A Addition compound of dipeptide derivative and amino acid derivative 失效
标题翻译：二肽衍生物和氨基酸衍生物的加成化合物
公开(公告)号：US4256836A
公开(公告)日：1981-03-17
申请号：US20058
申请日：1979-03-13
申请人： Yoshikazu Isowa , Muneki Ohmori , Kaoru Mori , Tetsuya Ichikawa , Yuji Nonaka , Keiichi Kihara , Kiyotaka Oyama , Heijiro Satoh , Shigeaki Nishimura
发明人： Yoshikazu Isowa , Muneki Ohmori , Kaoru Mori , Tetsuya Ichikawa , Yuji Nonaka , Keiichi Kihara , Kiyotaka Oyama , Heijiro Satoh , Shigeaki Nishimura
IPC分类号： C07K5/072 , C07K5/075 , C07C103/52 , C12P21/02
CPC分类号： C07K5/0613
摘要： Addition compounds having the formula ##STR1## wherein R.sub.1 represents an aliphatic oxycarbonyl group, benzyloxycarbonyl group which can have nuclear substituents, or benzoyl, aromatic sulfonyl or aromatic sulfinyl group; R.sub.2 represents methyl, isopropyl, isobutyl, isoamyl or benzyl group; R.sub.3 represents a lower alkoxyl, benzyloxy or benzhydryloxy group and n represents 1 or 2 are produced by reacting an N-substituted monoaminodicarboxylic acid having the formula ##STR2## with an amino carboxylic acid ester having the formula ##STR3## in the presence of a protease.
摘要翻译：具有式（I）的加成化合物，其中R 1表示脂族氧基羰基，可具有核取代基的苄氧基羰基，或苯甲酰基，芳族磺酰基或芳族亚磺酰基; R 2表示甲基，异丙基，异丁基，异戊基或苄基; R3表示低级烷氧基，苄氧基或二苯甲氧基，n表示1或2是通过使具有式（IV）的N-取代的单氨基二羧酸与具有式（VI）的氨基羧酸酯反应制备的，在蛋白酶的存在下。

5. 发明授权

US4165311A Addition compound of dipeptide derivative and amino acid derivative 失效
标题翻译：二肽衍生物和氨基酸衍生物的加成化合物
公开(公告)号：US4165311A
公开(公告)日：1979-08-21
申请号：US870108
申请日：1978-01-17
申请人： Yoshikazu Isowa , Muneki Ohmori , Kaoru Mori , Tetsuya Ichikawa , Yuji Nonaka , Keiichi Kihara , Kiyotaka Oyama , Heijiro Satoh , Shigeaki Nishimura
发明人： Yoshikazu Isowa , Muneki Ohmori , Kaoru Mori , Tetsuya Ichikawa , Yuji Nonaka , Keiichi Kihara , Kiyotaka Oyama , Heijiro Satoh , Shigeaki Nishimura
IPC分类号： C07K5/072 , C07K5/075 , C07C103/52 , C12B1/00
CPC分类号： C07K5/0613
摘要： Addition compounds having the formula ##STR1## wherein R.sub.1 represents an aliphatic oxycarbonyl group, benzyloxycarbonyl group which can have nuclear substituents, or benzoyl, aromatic sulfonyl or aromatic sulfinyl group; R.sub.2 represents methyl, isopropyl, isobutyl, isoamyl or benzyl group; R.sub.3 represents a lower alkoxyl, benzyloxy or benzhydryloxy group and n represents 1 or 2 together with methods for preparing (I) and for decomposing (I) into the constituent dipeptide esters and amino acid esters.
摘要翻译：具有式（I）的加成化合物，其中R 1表示脂族氧基羰基，可具有核取代基的苄氧基羰基，或苯甲酰基，芳族磺酰基或芳族亚磺酰基; R 2表示甲基，异丙基，异丁基，异戊基或苄基; R3表示低级烷氧基，苄氧基或二苯甲氧基，n表示1或2，以及用于制备（I）和用于将（I）分解成组分二肽酯和氨基酸酯的方法。

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