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1. 发明申请

US20090221429A1 Method for Detecting Target Nucleic Acid with Specific Base Sequence and Set of Nucleic Acids for Detection 有权
标题翻译：用特异性碱基序列和核酸组检测靶核酸的方法
公开(公告)号：US20090221429A1
公开(公告)日：2009-09-03
申请号：US12085101
申请日：2006-11-17
申请人： Kenzo Fujimoto , Yoshinaga Yoshimura , Shinzi Ogasawara
发明人： Kenzo Fujimoto , Yoshinaga Yoshimura , Shinzi Ogasawara
IPC分类号： C40B20/04 , G01N33/48 , G01N33/53
CPC分类号： C12Q1/6837 , C12Q1/6862 , Y10T436/143333 , C12Q2527/125 , C12Q2527/119 , C12Q2523/313 , C12Q2527/101
摘要： It is intended to provide a method for detecting a target nucleic acid with a specific base sequence existing in a sample mixture with high specificity and sensitivity by utilizing the formation of a hybrid with a complementary strand as a detection principle, and a nucleic acid for the detection. The invention relates to a set of nucleic acids for detecting a target nucleic acid with a specific base sequence comprising a photoligating nucleic acid composed of a nucleic acid (with the proviso that in the nucleic acid, a nucleic acid and a peptide nucleic acid are included) having a group represented by the formula I, II, III, IV or V described in claims at the 5′ end or 3′ end as a base moiety, and a photoligated nucleic acid having a base with a carbon-carbon double bond at the 3′ end or 5′ end as a base moiety capable of photoligating to the photoligating nucleic acid, wherein either of the photoligating nucleic acid and the photoligated nucleic acid has a labeling moiety and the other remaining nucleic acid is immobilized on a substrate, and the method for detecting a target nucleic acid with a specific base sequence by using the set of nucleic acids.
摘要翻译：旨在提供一种通过利用与互补链形成杂交体作为检测原理，以高特异性和灵敏度存在于样品混合物中的特异性碱基序列来检测靶核酸的方法，以及用于检测。本发明涉及一组用于检测具有特定碱基序列的靶核酸的核酸，所述特定碱基序列包含由核酸组成的光合核酸（条件是在核酸中包含核酸和肽核酸））具有由权利要求书中所述的在5'末端或3'末端作为碱基部分的式I，II，III，IV或V所代表的基团，以及具有碳 - 碳双键的碱基的光分解的核酸 3'末端或5'末端作为能够对光合核酸进行光电化的碱基部分，其中光电核酸和光动力核酸中的任一个具有标记部分，而另一个剩余的核酸固定在底物上，通过使用该组核酸检测具有特定碱基序列的靶核酸的方法。

2. 发明授权

US07851159B2 Method for detecting target nucleic acid with specific base sequence and set of nucleic acids for detection 有权
标题翻译：用特异性碱基序列和核酸组检测靶核酸进行检测的方法
公开(公告)号：US07851159B2
公开(公告)日：2010-12-14
申请号：US12085101
申请日：2006-11-17
申请人： Kenzo Fujimoto , Yoshinaga Yoshimura , Shinzi Ogasawara
发明人： Kenzo Fujimoto , Yoshinaga Yoshimura , Shinzi Ogasawara
IPC分类号： C12Q1/68 , C07H19/04 , C07H21/00 , C07H21/02
CPC分类号： C12Q1/6837 , C12Q1/6862 , Y10T436/143333 , C12Q2527/125 , C12Q2527/119 , C12Q2523/313 , C12Q2527/101
摘要： It is intended to provide a method for detecting a target nucleic acid with a specific base sequence existing in a sample mixture with high specificity and sensitivity by utilizing the formation of a hybrid with a complementary strand as a detection principle, and a nucleic acid for the detection. The invention relates to a set of nucleic acids for detecting a target nucleic acid with a specific base sequence comprising a photoligating nucleic acid composed of a nucleic acid (with the proviso that in the nucleic acid, a nucleic acid and a peptide nucleic acid are included) having a group represented by the formula I, II, III, IV or V described in claims at the 5′ end or 3′ end as a base moiety, and a photoligated nucleic acid having a base with a carbon-carbon double bond at the 3′ end or 5′ end as a base moiety capable of photoligating to the photoligating nucleic acid, wherein either of the photoligating nucleic acid and the photoligated nucleic acid has a labeling moiety and the other remaining nucleic acid is immobilized on a substrate, and the method for detecting a target nucleic acid with a specific base sequence by using the set of nucleic acids.
摘要翻译：旨在提供一种通过利用与互补链形成杂交体作为检测原理，以高特异性和灵敏度存在于样品混合物中的特异性碱基序列来检测靶核酸的方法，以及用于检测。本发明涉及一组用于检测具有特定碱基序列的靶核酸的核酸，所述特定碱基序列包含由核酸组成的光合核酸（条件是在核酸中包含核酸和肽核酸））具有由权利要求书中所述的在5'末端或3'末端作为碱基部分的式I，II，III，IV或V所代表的基团，以及具有碳 - 碳双键的碱基的光分解的核酸 3'末端或5'末端作为能够对光合核酸进行光电化的碱基部分，其中光电核酸和光动力核酸中的任一个具有标记部分，而另一个剩余的核酸固定在底物上，通过使用该组核酸检测具有特定碱基序列的靶核酸的方法。

3. 发明授权

US4223420A Hinge structure for automobile hoods 失效
标题翻译：汽车罩铰链结构
公开(公告)号：US4223420A
公开(公告)日：1980-09-23
申请号：US948916
申请日：1978-10-05
申请人： Kazuyoshi Yukimoto , Shinzi Ogasawara , Mitsuhiro Sakamoto , Yasunori Kawamura
发明人： Kazuyoshi Yukimoto , Shinzi Ogasawara , Mitsuhiro Sakamoto , Yasunori Kawamura
IPC分类号： B62D25/12 , E05D3/06 , E05F1/12 , E05D1/00
CPC分类号： E05D3/145 , E05F1/1276 , E05D11/06 , E05F5/06 , E05Y2201/21 , E05Y2201/218 , E05Y2201/26 , E05Y2800/748 , E05Y2900/50 , Y10S16/33 , Y10T16/53833
摘要： A hinge structure for automobile hoods, including a first bracket fixedly connected to the automobile hood, a second bracket fixedly connected to an automobile body, a link mechanism rotatably supporting both brackets by a link mechanism, an urging mechanism for urging the first bracket into the raising direction thereof, the hinge structure characterized in that a link connecting member having an elastic portion is fixed to the autobomile body at the rotating position of the link mechanism upon fully opening the automobile hood and the link mechanism is connected to the link connecting member by bending the elastic member.
摘要翻译：一种用于汽车罩的铰链结构，包括固定地连接到汽车罩的第一支架，固定地连接到汽车主体的第二支架，通过连杆机构可旋转地支撑两个支架的连杆机构，用于将第一支架推入铰链结构的特征在于，在完全打开汽车罩时，具有弹性部分的连杆连接件在连杆机构的旋转位置处固定到自动弹体体上，并且连杆机构通过连杆机构连接到连杆连接部件弯曲弹性构件。

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