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1. 发明申请

US20090166193A1 IMMOBILIZATION SUPPORT, PROCESS FOR PRODUCING THE SAME, ELECTRODE, PROCESS FOR PRODUCING THE SAME, ELECTRODE REACTION UTILIZING APPARATUS AND PROCESS FOR PRODUCING THE SAME 审中-公开
标题翻译：固化支持，其制造方法，电极，其制造方法，电极反应使用装置及其制造方法
公开(公告)号：US20090166193A1
公开(公告)日：2009-07-02
申请号：US12394476
申请日：2009-02-27
申请人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
发明人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
IPC分类号： C25B11/12
CPC分类号： C12Q1/003 , H01M4/86 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound.
摘要翻译：除了戊二醛和聚-L-赖氨酸之外，还使用含有电子受体化合物的固定载体，以将酶和电子受体化合物同时固定在电极上。例如，这里使用心肌黄酶作为酶和2-氨基-3-羧基-1,4-萘醌（ACNQ）作为电子受体化合物。

2. 发明授权

US09365882B2 Immobilization support, process for producing the same, electrode, process for producing the same, electrode reaction utilizing apparatus and process for producing the same 有权
公开(公告)号：US09365882B2
公开(公告)日：2016-06-14
申请号：US12394975
申请日：2009-02-27
申请人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
发明人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
IPC分类号： C12N11/00 , C12Q1/00 , H01M8/16 , H01M4/86
CPC分类号： C12Q1/003 , H01M4/86 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound.

3. 发明授权

US08236153B2 Immobilization support, process for producing the same, electrode, process for producing the same, electrode reaction utilizing apparatus and process for producing the same 有权
标题翻译：固定化载体，其制造方法，电极，其制造方法，电极反应利用装置及其制造方法
公开(公告)号：US08236153B2
公开(公告)日：2012-08-07
申请号：US12394730
申请日：2009-02-27
申请人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
发明人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
IPC分类号： C12M1/00 , C12Q1/00 , H01M2/16 , C12N11/06 , C12N11/14
CPC分类号： C12Q1/003 , H01M4/86 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1, 4-naphthoquinone (ACNQ) as the electron acceptor compound.
摘要翻译：除了戊二醛和聚-L-赖氨酸之外，还使用含有电子受体化合物的固定载体，以将酶和电子受体化合物同时固定在电极上。例如，这里使用心肌黄酶作为酶和2-氨基-3-羧基-1,4-萘醌（ACNQ）作为电子受体化合物。

4. 发明授权

US07520970B2 Immobilization support, process for producing the same, electrode, process for producing the same, electrode reaction utilizing apparatus and process for producing the same 有权
标题翻译：固定化载体，其制造方法，电极，其制造方法，电极反应利用装置及其制造方法
公开(公告)号：US07520970B2
公开(公告)日：2009-04-21
申请号：US10555368
申请日：2004-05-21
申请人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
发明人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
IPC分类号： C12M1/00 , C25B11/06 , C12Q1/68 , C12N11/08 , C12N1/00
CPC分类号： C12Q1/003 , H01M4/86 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound.
摘要翻译：除了戊二醛和聚-L-赖氨酸之外，还使用含有电子受体化合物的固定载体，以将酶和电子受体化合物同时固定在电极上。例如，这里使用心肌黄酶作为酶和2-氨基-3-羧基-1,4-萘醌（ACNQ）作为电子受体化合物。

5. 发明申请

US20090159438A1 IMMOBILIZATION SUPPORT, PROCESS FOR PRODUCING THE SAME, ELECTRODE, PROCESS FOR PRODUCING THE SAME, ELECTRODE REACTION UTILIZING APPARATUS AND PROCESS FOR PRODUCING THE SAME 有权
公开(公告)号：US20090159438A1
公开(公告)日：2009-06-25
申请号：US12394975
申请日：2009-02-27
申请人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
发明人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
IPC分类号： C25B11/12
CPC分类号： C12Q1/003 , H01M4/86 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound.

6. 发明申请

US20090166194A1 IMMOBILIZATION SUPPORT, PROCESS FOR PRODUCING THE SAME, ELECTRODE, PROCESS FOR PRODUCING THE SAME, ELECTRODE REACTION UTILIZING APPARATUS AND PROCESS FOR PRODUCING THE SAME 有权
标题翻译：固化支持，其制造方法，电极，其制造方法，电极反应使用装置及其制造方法
公开(公告)号：US20090166194A1
公开(公告)日：2009-07-02
申请号：US12394730
申请日：2009-02-27
申请人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
发明人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
IPC分类号： C25B11/12
CPC分类号： C12Q1/003 , H01M4/86 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1, 4-naphthoquinone (ACNQ) as the electron acceptor compound.
摘要翻译：除了戊二醛和聚-L-赖氨酸之外，还使用含有电子受体化合物的固定载体，以将酶和电子受体化合物同时固定在电极上。例如，这里使用心肌黄酶作为酶和2-氨基-3-羧基-1,4-萘醌（ACNQ）作为电子受体化合物。

7. 发明申请

US20060105418A1 Immobilization support, process for producing the same, electrode, process for producing the same, electrode reaction utilizing apparatus and process for producing the same 有权
公开(公告)号：US20060105418A1
公开(公告)日：2006-05-18
申请号：US10555368
申请日：2004-05-21
申请人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
发明人： Atsushi Sato , Tokuji Ikeda , Kenji Kano
IPC分类号： C12Q1/26
CPC分类号： C12Q1/003 , H01M4/86 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound.

8. 发明授权

US07514250B2 Coenzyme-binding glucose dehydrogenase 有权
标题翻译：辅酶结合葡萄糖脱氢酶
公开(公告)号：US07514250B2
公开(公告)日：2009-04-07
申请号：US10540025
申请日：2003-12-24
申请人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
IPC分类号： C12N9/02
CPC分类号： C12N9/0004 , C12Q1/32 , C12Q1/54
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.
摘要翻译：本发明提供了一种在电子受体存在下催化葡萄糖氧化反应的微生物衍生的可溶性辅酶结合葡萄糖脱氢酶，其麦芽糖活性低至5％以下，菲咯啉。本发明还提供了生产辅酶结合葡萄糖脱氢酶的方法，以及使用辅酶结合葡萄糖脱氢酶进行测定的方法和试剂。根据本发明，辅酶结合葡萄糖脱氢酶可以应用于工业领域，并且也可以在材料生产或分析中使用，包括使用辅酶结合葡萄糖脱氢酶作为测定或消除样品中的葡萄糖的方法以及作为有机化合物的制造方法。还可以提供能够精确测量血糖水平的葡萄糖传感器。因此，可以提供具有高效用的酶，例如用于改变药物，临床研究和食品领域的材料的能力。

9. 发明申请

US20100297743A1 COENZYME-BINDING GLUCOSE DEHYDROGENASE 审中-公开
标题翻译：聚苯乙烯葡萄糖脱氢酶
公开(公告)号：US20100297743A1
公开(公告)日：2010-11-25
申请号：US12851668
申请日：2010-08-06
申请人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
IPC分类号： C12M1/34
CPC分类号： C12N9/0004 , C12Q1/32 , C12Q1/54
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.
摘要翻译：本发明提供了一种在电子受体存在下催化葡萄糖氧化反应的微生物衍生的可溶性辅酶结合葡萄糖脱氢酶，其麦芽糖活性低至5％以下，菲咯啉。本发明还提供了生产辅酶结合葡萄糖脱氢酶的方法，以及使用辅酶结合葡萄糖脱氢酶进行测定的方法和试剂。根据本发明，辅酶结合葡萄糖脱氢酶可以应用于工业领域，并且也可以在材料生产或分析中使用，包括使用辅酶结合葡萄糖脱氢酶作为测定或消除样品中的葡萄糖的方法以及作为有机化合物的制造方法。还可以提供能够精确测量血糖水平的葡萄糖传感器。因此，可以提供具有高效用的酶，例如用于改变药物，临床研究和食品领域的材料的能力。

10. 发明申请

US20060063217A1 Coenzyme-binding glucose dehydrogenase 有权
公开(公告)号：US20060063217A1
公开(公告)日：2006-03-23
申请号：US10540025
申请日：2003-12-24
申请人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
IPC分类号： C12Q1/54 , C12N9/02 , C12N1/16
CPC分类号： C12N9/0004 , C12Q1/32 , C12Q1/54
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

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