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    • 2. 发明申请
    • ISOTACHOPHORESIS
    • US20100270157A1
    • 2010-10-28
    • US12709423
    • 2010-02-19
    • Tatsuo KurosawaMitsuo WatanabeTakuma Ohtsubo
    • Tatsuo KurosawaMitsuo WatanabeTakuma Ohtsubo
    • G01N27/447
    • G01N27/44773G01N27/44726
    • Problems to be SolvedThere is provided a method for separating the complex containing the analyte (or the analogue) in the blood-derived sample and the labeling substances, etc., and coexisting substances in a blood-derived sample, rapidly, simply and conveniently and in high precision by a isotachophoresis (ITP); and a measuring method for the analyte in said sample in high precision and in high sensitivity, based on amount of the complex separated or amount of the free labeling substance-containing molecules, which were not involved in formation of said complex.Method for Solving the ProblemA method for separating a complex which comprises, concentrating the following complex, while separating a complex containing an analyte (or the analogue) in the blood-derived sample, the substances which are capable of forming the complex (CFS) with said analyte and the substances which are capable of changing electrophoretic mobility of the analyte (or the analogue), from the free labeling substance-containing molecules (the labeled CFS, the labeled analogue and the analyte-labeled CFS complex) which were not involved in formation of the complex including the labeling substances and the coexisting substances in said sample, by ITP, in the presence of an 2-(N-morpholino)ethane sulfonate (MES) ion and/or a glutamate ion.
    • 要解决的问题提供一种方法,用于将血源性样品中的分析物(或类似物)和标记物质等的复合物以及血液样品中的共存物质快速,简便地 并通过等速电泳(ITP)以高精度进行; 以及基于未参与形成所述复合物的含有分子量或含游离标记物质的分子的量,以高精度和高灵敏度的方式分析所述样品中的分析物。 解决问题的方法一种分离复合物的方法,其包括在分离含有来自血液的样品中的分析物(或类似物)的复合物的同时浓缩以下复合物,能够形成复合物(CFS)的物质, 所述分析物和能够从未涉及的游离标记物质的分子(标记的CFS,标记的类似物和分析物标记的CFS复合物)改变分析物(或类似物)的电泳迁移率的物质 通过ITP在2-(N-吗啉代)乙烷磺酸盐(MES)离子和/或谷氨酸根离子的存在下形成包含所述样品中的标记物质和共存物质的复合物。