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1. 发明申请

US20070067861A1 Cloning pigs using donor nuclei from differentiated cells 审中-公开
标题翻译：使用来自分化细胞的供体核克隆猪
公开(公告)号：US20070067861A1
公开(公告)日：2007-03-22
申请号：US11603036
申请日：2006-11-22
申请人： Steven Stice , James Robl , Jose Cibelli , Paul Golueke
发明人： Steven Stice , James Robl , Jose Cibelli , Paul Golueke
IPC分类号： A01K67/027
CPC分类号： C12N15/8778 , A01K67/027 , A01K67/0273 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A61K35/12 , A61K38/00 , C12N5/0603 , C12N15/8771 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的猪细胞核移植到去核猪卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生来增殖基因型和转基因基因型。通过本发明的方法促进转基因植物或转基因猪胚胎，胎儿和后代的生产，因为供体核的分化细胞源可被遗传修饰和克隆繁殖。

2. 发明申请

US20060021070A1 Production of chimeric bovine or porcine animals using cultured inner cell mass 审中-公开
标题翻译：使用培养的内细胞块生产嵌合牛或猪动物
公开(公告)号：US20060021070A1
公开(公告)日：2006-01-26
申请号：US11024010
申请日：2004-12-28
申请人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Leon , D. Jerry
发明人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Leon , D. Jerry
IPC分类号： A01K67/027
CPC分类号： C12N15/8771 , A01K2217/05 , C12N15/8778
摘要： Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar morphology and express cell markers identically or substantially similarly to ICMs of undifferentiated developing embryos for prolonged culturing periods. Heterologous DNA is inserted into the subject CICM cells and cell lines so as produce transgenic CICM cell which are introduced into non-human fertilized embryos to produce transgenic chimeric embryos. The transgenic chimeric embryos are transferred into recipient females where they are permitted to develop into transgenic chimeric fetuses. Recipient females give birth to transgenic chimeric animals which are capable of transmitting the heterologous DNA to their progeny. Transgenic CICM cells are also used to produce cloned transgenic embryos, fetuses and offspring.
摘要翻译：提供了新颖的培养的内细胞团（CICM）细胞和源自有蹄类动物，特别是猪和牛的细胞系及其制备方法。受试者CICM具有与未分化发育的胚胎的ICM相似或基本上类似于延长培养期的相似形态和表达细胞标记。将异源DNA插入受试者CICM细胞和细胞系中，以产生转入非人受精胚胎以产生转基因嵌合胚胎的转基因CICM细胞。将转基因嵌合胚胎转移到受体雌性中，在其中允许其转化为转基因嵌合胎儿。受体女性产生能够将异源DNA转移到其后代的转基因嵌合动物。转基因CICM细胞也用于产生克隆的转基因胚胎，胎儿和后代。

3. 发明申请

US20050108785A1 Cloning using donor nuclei from differentiated fetal and adult cells 审中-公开
标题翻译：使用来自分化胎儿和成体细胞的供体核克隆
公开(公告)号：US20050108785A1
公开(公告)日：2005-05-19
申请号：US10932921
申请日：2004-09-02
申请人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. de Leon , D. Jerry
发明人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. de Leon , D. Jerry
IPC分类号： A01K67/02 , A01K67/027 , A61K35/12 , A61P1/18 , A61P3/10 , A61P9/00 , A61P9/04 , A61P13/02 , A61P17/02 , A61P21/00 , A61P25/16 , A61P25/28 , A61P31/18 , A61P35/00 , C12N5/073 , C12N5/10 , C12N15/09 , C12N15/877
CPC分类号： A01K67/0275 , A01K67/027 , A01K2217/05 , A01K2227/101 , A01K2227/106 , A61K35/12 , C12N5/0603 , C12N15/8771 , C12N15/8778 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的细胞核移植到与供体细胞相同物种的去核卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生以及用于产生包括人同种异体胚胎或干细胞在内的等基因CICM细胞的基因型和转基因基因型的倍增。通过本方法促进遗传工程或转基因哺乳动物胚胎，胎儿和后代的生产，因为供体核的分化细胞源可以被遗传修饰和克隆繁殖。

4. 发明申请

US20120076761A1 Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including Parkinson's disease 审中-公开
标题翻译：克隆有蹄类动物的胚胎和动物，使用细胞，组织和器官进行移植治疗，包括帕金森病
公开(公告)号：US20120076761A1
公开(公告)日：2012-03-29
申请号：US12896396
申请日：2010-10-01
申请人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce De Leon , D. Joseph Jerry
发明人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce De Leon , D. Joseph Jerry
IPC分类号： A61K35/30 , C12N5/0793 , A61P25/16 , A61P37/06 , C12N5/079 , C12N5/10
CPC分类号： A01K67/02 , A01K67/027 , A01K67/0271 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A61K35/12 , A61K35/30 , A61K38/00 , C12N5/0603 , C12N15/8771 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： Methods and cell lines for cloning ungulate embryos and offspring, in particular bovines and porcines, are provided. The resultant fetuses, embryos or offspring are especially useful for the expression of desired heterologous DNAs, and may be used as a source of cells or tissue for transplantation therapy for the treatment of diseases such as Parkinson's disease.
摘要翻译：提供用于克隆有蹄类胚胎和后代的方法和细胞系，特别是牛和猪。所产生的胎儿，胚胎或后代对于所需的异源DNA的表达特别有用，并且可以用作用于治疗诸如帕金森病之类疾病的移植治疗的细胞或组织来源。

5. 发明申请

US20090092588A1 Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease 审中-公开
标题翻译：克隆有蹄类动物的胚胎和动物，使用细胞，组织和器官进行移植治疗，包括帕金森病
公开(公告)号：US20090092588A1
公开(公告)日：2009-04-09
申请号：US11529720
申请日：2006-09-26
申请人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce De Leon , D. Joseph Jerry
发明人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce De Leon , D. Joseph Jerry
IPC分类号： A61K35/12 , C12N15/87 , C12N5/08
CPC分类号： A01K67/02 , A01K67/027 , A01K67/0271 , A01K67/0273 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A61K35/12 , A61K35/30 , A61K38/00 , C12N5/0603 , C12N15/8771 , C12N15/8778 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： Methods and cell lines for cloning ungulate embryos and offspring, in particular bovines and porcines, are provided. The resultant fetuses, embryos or offspring are especially useful for the expression of desired heterologous DNAs, and may be used as a source of cells or tissue for transplantation therapy for the treatment of diseases such as Parkinson's disease.
摘要翻译：提供用于克隆有蹄类胚胎和后代的方法和细胞系，特别是牛和猪。所产生的胎儿，胚胎或后代对于所需的异源DNA的表达特别有用，并且可以用作用于治疗诸如帕金森病之类疾病的移植治疗的细胞或组织来源。

6. 发明授权

US06235970B1 CICM cells and non-human mammalian embryos prepared by nuclear transfer of a proliferating differentiated cell or its nucleus 失效
标题翻译：通过增殖分化细胞或其核的核转移制备的CICM细胞和非人哺乳动物胚胎
公开(公告)号：US06235970B1
公开(公告)日：2001-05-22
申请号：US08935052
申请日：1997-09-22
申请人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
发明人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
IPC分类号： C12N1500
CPC分类号： A01K67/0275 , A01K67/027 , A01K2217/05 , A01K2227/101 , A01K2227/106 , A61K35/12 , C12N5/0603 , C12N15/8771 , C12N15/8778 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的细胞核移植到与供体细胞相同物种的去核卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生以及用于产生包括人同种异体胚胎或干细胞在内的等基因CICM细胞的基因型和转基因基因型的倍增。通过本方法促进遗传工程或转基因哺乳动物胚胎，胎儿和后代的生产，因为供体核的分化细胞源可以被遗传修饰和克隆繁殖。

7. 发明授权

US5945577A Cloning using donor nuclei from proliferating somatic cells 失效
标题翻译：使用供体核从增殖体细胞克隆
公开(公告)号：US5945577A
公开(公告)日：1999-08-31
申请号：US781752
申请日：1997-01-10
申请人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
发明人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
IPC分类号： A01K67/02 , A01K67/027 , A61K35/12 , A61P1/18 , A61P3/10 , A61P9/00 , A61P9/04 , A61P13/02 , A61P17/02 , A61P21/00 , A61P25/16 , A61P25/28 , A61P31/18 , A61P35/00 , C12N5/073 , C12N5/10 , C12N15/09 , C12N15/877 , C12N5/00 , C12N15/00 , A01K67/00
CPC分类号： A01K67/0275 , A01K67/027 , C12N15/8771 , C12N15/8778 , C12N5/0603 , A01K2217/05 , A01K2227/101 , A01K2227/106 , A61K35/12 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的细胞核移植到与供体细胞相同物种的去核卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生以及用于产生包括人同种异体胚胎或干细胞在内的等基因CICM细胞的基因型和转基因基因型的倍增。通过本方法促进遗传工程或转基因哺乳动物胚胎，胎儿和后代的生产，因为供体核的分化细胞源可以被遗传修饰和克隆繁殖。

8. 发明授权

US07291764B1 Cloning pigs using non-quiescent differentiated donor cells or nuclei 有权
标题翻译：使用非静止分化供体细胞或细胞核克隆猪
公开(公告)号：US07291764B1
公开(公告)日：2007-11-06
申请号：US09394902
申请日：1999-09-13
申请人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke
发明人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke
IPC分类号： C12N15/00 , C12N5/00 , A01K67/00
CPC分类号： C12N15/8778
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的猪细胞核移植到去核猪卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生来增殖基因型和转基因基因型。通过本发明的方法促进转基因植物或转基因猪胚胎，胎儿和后代的生产，因为供体核的分化细胞源可被遗传修饰和克隆繁殖。

9. 发明申请

US20050250203A1 Embryonic or stem-like cell lines produced by cross species nuclear transplantation 审中-公开
公开(公告)号：US20050250203A1
公开(公告)日：2005-11-10
申请号：US10981137
申请日：2004-11-04
申请人： James Robl , Jose Cibelli , Steven Stice
发明人： James Robl , Jose Cibelli , Steven Stice
IPC分类号： C12N15/09 , A61K35/12 , A61K38/00 , A61K48/00 , A61P1/16 , A61P3/10 , A61P9/00 , A61P13/00 , A61P25/16 , A61P25/28 , A61P31/18 , A61P35/00 , C12N5/10 , C12N15/85 , C12N15/87 , C12N15/877 , C12N5/08
CPC分类号： C12N15/8776 , A61K35/12 , A61K38/00 , C12N15/87 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation.

10. 发明授权

US5994619A Production of chimeric bovine or porcine animals using cultured inner cell mass cells 失效
标题翻译：使用培养的内细胞大量细胞生产嵌合牛或猪动物
公开(公告)号：US5994619A
公开(公告)日：1999-11-30
申请号：US766939
申请日：1996-12-16
申请人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
发明人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
IPC分类号： C12N15/09 , A01K67/027 , C12N5/073 , C12N5/0735 , C12N5/10 , C12N15/06 , C12N15/877 , C12N5/00 , C12N15/00 , C12N15/63
CPC分类号： A01K67/0275 , C12N15/8771 , C12N5/0603 , C12N5/0606 , A01K2217/05 , C12N2501/235 , C12N2502/13 , C12N2510/00
摘要： Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar morphology and express cell markers identically or substantially similarly to ICMs of undifferentiated developing embryos for prolonged culturing periods. Heterologous DNA is inserted into the subject CICM cells and cell lines so as produce transgenic CICM cell which are introduced into non-human fertilized embryos to produce transgenic chimeric embryos. The transgenic chimeric embryos are transferred into recipient females where they are permitted to develop into transgenic chimeric fetuses. Recipient females give birth to transgenic chimeric animals which are capable of transmitting the heterologous DNA to their progeny. Transgenic CICM cells are also used to produce cloned transgenic embryos, fetuses and offspring.
摘要翻译：提供了新颖的培养的内细胞团（CICM）细胞和源自有蹄类动物，特别是猪和牛的细胞系及其制备方法。受试者CICM具有与未分化发育的胚胎的ICM相似或基本上类似于延长培养期的相似形态和表达细胞标记。将异源DNA插入受试者CICM细胞和细胞系中，以产生转入非人受精胚胎以产生转基因嵌合胚胎的转基因CICM细胞。将转基因嵌合胚胎转移到受体雌性中，在其中允许其转化为转基因嵌合胎儿。受体女性产生能够将异源DNA转移到其后代的转基因嵌合动物。转基因CICM细胞也用于产生克隆的转基因胚胎，胎儿和后代。

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