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    • 3. 发明授权
    • Live-cell biosensor polypeptides and methods of use
    • 活细胞生物传感器多肽和使用方法
    • US07655434B2
    • 2010-02-02
    • US11052001
    • 2005-02-03
    • Joshua T. JonesAngela Teresa HahnTobias Meyer
    • Joshua T. JonesAngela Teresa HahnTobias Meyer
    • C12Q1/02C12N5/06
    • G01N33/502G01N33/5008G01N33/582
    • Methods and compositions for determining the duration of a cell cycle phase of a mammalian cell, as well as identification of the cell cycle stage of fixed mammalian cells, are provided. In practicing the subject methods, at least one biosensor polypeptide that monitors a cell-cycle phase in a mammalian cell, such as mitosis, G1, S, or G2 phase, is used to determine the duration of a cell-cycle phase of a mammalian cell. Also provided are methods for identifying an agent (e.g., a gene product or small molecule compound) that modulates the duration of a cell-cycle phase of a mammalian cell, as well as kits and systems for practicing the subject methods.
    • 提供了用于确定哺乳动物细胞的细胞周期阶段的持续时间的方法和组合物,以及固定哺乳动物细胞的细胞周期阶段的鉴定。 在实施本发明方法中,使用至少一种监测哺乳动物细胞(例如有丝分裂,G1,S或G2期)中的细胞周期相的生物传感器多肽来确定哺乳动物的细胞周期阶段的持续时间 细胞。 还提供了用于鉴定调节哺乳动物细胞的细胞周期阶段的持续时间的试剂(例如,基因产物或小分子化合物)以及用于实践本发明方法的试剂盒和系统的方法。
    • 8. 发明授权
    • Method of introducing exogenous compounds into cells by electroporation and apparatus for same
    • 通过电穿孔将外源化合物引入细胞的方法及其装置
    • US06261815B1
    • 2001-07-17
    • US09227684
    • 1999-01-08
    • Tobias Meyer
    • Tobias Meyer
    • C12N1300
    • C12N15/87C12M35/02
    • An electroporation apparatus for introducing exogenous material into cells is described herein. The apparatus comprises first a base member configured for holding a cell support, the cell support having a top surface portion, with the top surface portion configured for carrying adherent cells. The apparatus further comprises an electrode carrier operably associated with the base member, the electrode carrier having a bottom surface portion, a first electrode connected to the electrode carrier, and a second electrode also connected to the electrode carrier. The electrode carrier has a channel formed therein, with the channel positioned between the first electrode and the second electrode, so that exogenous material may be introduced through the channel and into contact with the cells. Methods for introducing exogenous compounds into a cell and for visually detecting the location of binding events within a cell are also disclosed.
    • 本文描述了用于将外源材料引入细胞的电穿孔装置。 该装置包括首先构造成用于保持细胞支撑体的基底构件,所述细胞支撑体具有顶表面部分,顶表面部分配置用于携带贴壁细胞。 所述装置还包括可操作地与所述基底构件相关联的电极载体,所述电极载体具有底表面部分,连接到所述电极载体的第一电极和还连接到所述电极载体的第二电极。 电极载体具有形成在其中的通道,通道位于第一电极和第二电极之间,使得外源材料可以通过通道引入并与电池接触。 还公开了将外源化合物引入细胞并用于目视检测细胞内结合事件的位置的方法。
    • 9. 发明授权
    • Method of introducing exogenous compounds into cells by electroporation
and apparatus for same
    • 通过电穿孔将外源化合物引入细胞的方法及其装置
    • US5874268A
    • 1999-02-23
    • US718658
    • 1996-09-23
    • Tobias Meyer
    • Tobias Meyer
    • C12M3/00C12N15/87C12N13/00
    • C12N15/87C12M35/02
    • An electroporation apparatus for introducing exogenous material into cells is described herein. The apparatus comprises first a base member (15) configured for holding a cell support, the cell support having a top surface portion, with the top surface portion configured for carrying adherent cells. The apparatus further comprises an electrode carrier (25) operably associated with the base member, the electrode carrier having a bottom surface portion, a first electrode (30) connected to the electrode carrier, and a second electrode (35) also connected to the electrode carrier. The electrode carrier has a channel (40) formed therein, with the channel positioned between the first electrode and the second electrode, so that exogenous material may be introduced through the channel and into contact with the cells. Methods for introducing exogenous compounds into a cell and for visually detecting the location of binding events within a cell are also disclosed.
    • 本文描述了用于将外源材料引入细胞的电穿孔装置。 该装置包括第一基座构件(15),其构造成用于保持单元支撑件,所述单元支撑件具有顶表面部分,顶表面部分配置用于承载贴壁单元。 所述装置还包括可操作地与所述基底构件相关联的电极载体(25),所述电极载体具有底表面部分,连接到所述电极载体的第一电极(30)和还连接到所述电极的第二电极 载体 电极载体具有形成在其中的通道(40),通道位于第一电极和第二电极之间,使得外源材料可以通过通道引入并与电池接触。 还公开了将外源化合物引入细胞并用于目视检测细胞内结合事件的位置的方法。