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1. 发明授权

US09512171B2 Functional molecule and manufacturing method therefor 有权
标题翻译：功能分子及其制造方法
公开(公告)号：US09512171B2
公开(公告)日：2016-12-06
申请号：US12929359
申请日：2011-01-19
申请人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
发明人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
IPC分类号： C40B30/04 , C07K1/107 , C07K1/04 , C07K1/22
CPC分类号： C07K1/1077 , C07K1/04 , C07K1/22
摘要： A novel method for efficiently manufacturing a functional molecule with affinity for a target substance is provided along with a functional molecule manufactured by this method. The method for manufacturing a functional molecule includes binding, to a resin, a specific substance having a cleavable linker which is a site that is cleavable under a specific condition to prepare a specific-substance-bound resin, treating this specific-substance-bound resin with a solution comprising functional molecule candidates, and then cleaving the cleavable linker under the specific condition to select a functional molecule which has interacted specifically with the specific substance.
摘要翻译：与通过该方法制造的功能分子一起提供了对目标物质具有亲和性的功能性分子的有效制造的新颖方法。功能分子的制造方法包括将具有可切割接头的特定物质与特定物质结合的树脂结合，所述可切割接头是在特定条件下可切割的部位，处理该特定物质结合树脂用包含功能性分子候选物的溶液，然后在特定条件下切割可切割接头以选择与特异性物质特异性相互作用的功能性分子。

2. 发明授权

US07759473B2 Nucleotide oligomer, nucleotide polymer, method for determining structure of functional substance and method for manufacturing functional substance 有权
标题翻译：核苷酸寡聚物，核苷酸聚合物，功能性物质结构的测定方法及功能性物质的制造方法
公开(公告)号：US07759473B2
公开(公告)日：2010-07-20
申请号：US11352197
申请日：2006-02-13
申请人： Tsuyoshi Fujihara , Shozo Fujita
发明人： Tsuyoshi Fujihara , Shozo Fujita
IPC分类号： C07H21/02 , C07H21/04
CPC分类号： C07H21/00
摘要： A modified nucleotide n-mer (where n is an integer of 2 or more) is used which comprises a nucleoside unit with a substituent group introduced into the base, wherein the substituent group is bound to the base via a triple bond. Novel nucleotide oligomers, nucleotide polymers, and nucleosides which can be used as raw materials or intermediates in the synthesis of this nucleotide oligomer and nucleotide polymer, as well as novel techniques for structural determination and manufacture of a functional substance having high affinity for a target, are provided.
摘要翻译：使用修饰的核苷酸n-mer（其中n为2以上的整数），其包含具有引入碱中的取代基的核苷单元，其中所述取代基通过三键与碱结合。可用作合成该核苷酸寡聚体和核苷酸聚合物的原料或中间体的新型核苷酸低聚物，核苷酸聚合物和核苷，以及用于结构测定和制造对靶具有高亲和力的功能物质的新技术，被提供。

3. 发明申请

US20100144060A1 Protein detecting device 有权
标题翻译：蛋白检测装置
公开(公告)号：US20100144060A1
公开(公告)日：2010-06-10
申请号：US12654137
申请日：2009-12-11
申请人： Shozo Fujita , Shunsaku Takeishi , Tsuyoshi Fujihara
发明人： Shozo Fujita , Shunsaku Takeishi , Tsuyoshi Fujihara
IPC分类号： G01N33/543
CPC分类号： G01N33/5438 , G01N33/53 , G01N33/542 , G01N33/54353 , G01N33/68
摘要： A protein detecting device, which comprises: (1) a detecting unit having a bonding section, which has properties for specifically bonding to a protein to be detected, a detecting section for detecting the bonding of the protein to be detected to the bonding section, the detecting section being made up of a polynucleotide double strand and a charge separating group, and an electrode section detecting the change in electrical conductivity of, or amount of transferred charge in, the polynucleotide double strand modified by the bond of the protein, (2) a standard electrode, (3) a reference electrode, (4) a container for housing the detecting unit, the standard electrode and the reference electrode, and containing a sample solutions comprising the protein to be detected, and (5) a measuring unit for measuring the protein based on a signal detected in the detecting unit.
摘要翻译：一种蛋白质检测装置，其特征在于，包括：（1）检测单元，具有具有特异性地结合待检测蛋白质的接合部的检测部，检测所述被检测蛋白质与接合部的接合的检测部，检测部分由多核苷酸双链和电荷分离基团组成，电极部分检测由蛋白质键修饰的多核苷酸双链中的导电性或转移电荷量的变化（2 ）标准电极，（3）参考电极，（4）容纳检测单元，标准电极和参比电极的容器，并包含含有待检测蛋白质的样品溶液，和（5）测量单元用于基于在检测单元中检测到的信号测量蛋白质。

4. 发明授权

US07517646B2 Functional molecule and process for producing the same 有权
标题翻译：功能分子及其制备方法
公开(公告)号：US07517646B2
公开(公告)日：2009-04-14
申请号：US10943150
申请日：2004-09-17
申请人： Tsuyoshi Fujihara , Shozo Fujita , Shunsaku Takeishi
发明人： Tsuyoshi Fujihara , Shozo Fujita , Shunsaku Takeishi
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/00 , C07H21/02 , C07H19/04
CPC分类号： A61K31/7115 , A61K48/00 , C07H21/00 , C12N15/1048 , C12N15/1058 , C12N15/115 , C12N2310/53
摘要： A process for producing a functional molecule includes a forming step which forms a modified nucleotide n-mer (where, n represents an integer) containing a modified nucleoside prepared by introducing a substituent into a nucleoside composing a nucleic acid; and a producing step which produces a modified oligonucleotide sequence by randomly polymerizing the modified nucleotide n-mer. A preferable embodiment thereof includes a selecting step which selects a sequence having an affinity to a target from the modified oligonucleotide sequence, a determining step which amplifies the selected modified oligonucleotide sequence and determines the base sequence thereof, and a translating step which translates the sequence of the modified oligonucleotide sequence on the basis of a relation table prepared by relating at least one of 4n kinds of nucleotide n-mers, which are presented in the relation table prepared by the one-to-one combination of 4 kinds of nucleosides, to a modified nucleotide n-mer.
摘要翻译：制备功能性分子的方法包括：形成步骤，其形成含有通过将取代基引入构成核酸的核苷中制备的修饰核苷的修饰的核苷酸n-mer（其中，n表示整数）; 以及通过随机聚合修饰的核苷酸n-mer产生修饰的寡核苷酸序列的制备步骤。其优选实施方案包括从修饰的寡核苷酸序列中选择与靶具有亲和性的序列的选择步骤，放大所选择的修饰寡核苷酸序列并确定其碱基序列的确定步骤，以及翻译步骤，基于通过将通过将4种核苷的一对一组合制备的关系表中存在的4n种核苷酸n-mers中的至少一种与至少一种核苷酸n-mers相关联的关系表，修饰的寡核苷酸序列与修饰的核苷酸n-mer。

5. 发明授权

US08012720B2 Protein detecting device 有权
标题翻译：蛋白检测装置
公开(公告)号：US08012720B2
公开(公告)日：2011-09-06
申请号：US12654137
申请日：2009-12-11
申请人： Shozo Fujita , Shunsaku Takeishi , Tsuyoshi Fujihara
发明人： Shozo Fujita , Shunsaku Takeishi , Tsuyoshi Fujihara
IPC分类号： C12P19/34
CPC分类号： G01N33/5438 , G01N33/53 , G01N33/542 , G01N33/54353 , G01N33/68
摘要： A protein detecting device, which comprises: (1) a detecting unit having a bonding section, which has properties for specifically bonding to a protein to be detected, a detecting section for detecting the bonding of the protein to be detected to the bonding section, the detecting section being made up of a polynucleotide double strand and a charge separating group, and an electrode section detecting the change in electrical conductivity of, or amount of transferred charge in, the polynucleotide double strand modified by the bond of the protein, (2) a standard electrode, (3) a reference electrode, (4) a container for housing the detecting unit, the standard electrode and the reference electrode, and containing a sample solutions comprising the protein to be detected, and (5) a measuring unit for measuring the protein based on a signal detected in the detecting unit.
摘要翻译：一种蛋白质检测装置，其特征在于，包括：（1）检测单元，具有具有特异性地结合待检测蛋白质的接合部的检测部，检测所述被检测蛋白质与接合部的接合的检测部，检测部分由多核苷酸双链和电荷分离基团组成，电极部分检测由蛋白质键修饰的多核苷酸双链中的导电性或转移电荷量的变化（2 ）标准电极，（3）参考电极，（4）容纳检测单元，标准电极和参比电极的容器，并包含含有待检测蛋白质的样品溶液，和（5）测量单元用于基于在检测单元中检测到的信号测量蛋白质。

6. 发明授权

US07723495B2 Amidite for nucleic acid synthesis and nucleic acid synthesizing method 有权
标题翻译：氨脒用于核酸合成和核酸合成方法
公开(公告)号：US07723495B2
公开(公告)日：2010-05-25
申请号：US11987168
申请日：2007-11-28
申请人： Tsuyoshi Fujihara , Shozo Fujita
发明人： Tsuyoshi Fujihara , Shozo Fujita
IPC分类号： C07H19/00 , C07H21/00
CPC分类号： C07H19/02 , Y02P20/55
摘要： To provide an amidite for nucleic acid synthesis, which enables a protective group therein to be removed under moderate conditions and can be practically used, and a nucleic acid synthesizing method using the amidite for nucleic acid synthesis. Specifically, the present invention relates to an amidite for nucleic acid synthesis represented by General Formula (I) below, and a nucleic acid synthesizing method using the amidite for nucleic acid synthesis: where X denotes a base; Y denotes a protective group formed of any one of a 4-aminobutyric acid derivative, an o-aminomethylbenzoic acid derivative, an o-aminophenylacetic acid derivative, an o-aminoethylbenzoic acid derivative, an o-aminomethylphenylacetic acid derivative, an o-aminophenylpropionic acid derivative and a 5-aminovaleric acid derivative; and Q denotes one of a hydrogen atom and a hydroxyl group.
摘要翻译：提供用于核酸合成的脒基，其使得其中的保护基能够在中等条件下除去并且可以实际使用，以及使用脒矿进行核酸合成的核酸合成方法。具体而言，本发明涉及下述通式（I）表示的核酸合成用脒盐，以及使用该酰胺化物进行核酸合成的核酸合成方法，其中X表示碱; Y表示由4-氨基丁酸衍生物，邻氨基甲基苯甲酸衍生物，邻氨基苯乙酸衍生物，邻氨基乙基苯甲酸衍生物，邻氨基甲基苯乙酸衍生物，邻氨基苯基丙酸衍生物和5-氨基戊酸衍生物; Q表示氢原子和羟基中的一个。

7. 发明申请

US20070232793A1 Functional molecule and manufacturing method therefor 审中-公开
公开(公告)号：US20070232793A1
公开(公告)日：2007-10-04
申请号：US11727390
申请日：2007-03-26
申请人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
发明人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
IPC分类号： C07K1/22 , C07H21/04
CPC分类号： C07K1/1077 , C07K1/04 , C07K1/22
摘要： A novel method for efficiently manufacturing a functional molecule with affinity for a target substance is provided along with a functional molecule manufactured by this method. The method for manufacturing a functional molecule includes binding, to a resin, a specific substance having a cleavable linker which is a site that is cleavable under a specific condition to prepare a specific-substance-bound resin, treating this specific-substance-bound resin with a solution comprising functional molecule candidates, and then cleaving the cleavable linker under the specific condition to select a functional molecule which has interacted specifically with the specific substance.

8. 发明申请

US20060040378A1 Device and method for quantitatively determining an analyte, a method for determining an effective size of a molecule, a method for attaching molecules to a substrate, and a device for detecting molecules 有权
公开(公告)号：US20060040378A1
公开(公告)日：2006-02-23
申请号：US11036367
申请日：2005-01-18
申请人： Kenji Arinaga , Ulrich Rant , Tsuyoshi Fujihara , Shozo Fujita
发明人： Kenji Arinaga , Ulrich Rant , Tsuyoshi Fujihara , Shozo Fujita
IPC分类号： C12Q1/68 , G01N33/53 , C12M1/34
CPC分类号： G01N33/5438 , B01J2219/00605 , C12Q2527/137 , C12Q2527/143 , C12Q2565/518 , G01N33/543
摘要： A device for quantitatively determining an analyte is provided to conspicuously improve the performance of the quantitative determination. This device is equipped with a flow channel, an analyte detecting unit for capturing and detecting the analyte, and a quantitative measurement unit for quantitatively determining the analyte, wherein a signal generated when the analyte detecting unit has detected the analyte is divided into a plurality of parts in the direction of the flow in the flow channel at the quantitative measurement unit for processing. Also provided are technologies including one for controlling the density of molecules attached to the surface of a solid. In these technologies, when molecules are attached to a substrate, the density of attached molecules is controlled, by having an electrolyte also present in a solution containing the molecules to adjust the screening effect by the electrolyte, and by taking into consideration the effective size of a molecule.

9. 发明授权

US08288516B2 Functional molecule, functional molecule synthesizing amidite and target substance analysis method 有权
标题翻译：功能分子，功能分子合成酰胺和目标物质分析方法
公开(公告)号：US08288516B2
公开(公告)日：2012-10-16
申请号：US12049825
申请日：2008-03-17
申请人： Tsuyoshi Fujihara , Shozo Fujita
发明人： Tsuyoshi Fujihara , Shozo Fujita
IPC分类号： C07H21/02 , C07H21/04 , C07H19/04 , C12Q1/68
CPC分类号： C12Q1/6806 , C07H21/04
摘要： To provide a functional molecule including a modified nucleotide unit having a substituent introduced to a base thereof, wherein the substituent is removably introduced to the base; a functional molecule synthesizing amidite that has a substituent removably introduced to its base and that is used for the manufacture of the functional molecule; and a target substance analysis method including: preparing a random pool of functional molecules using a functional molecule synthesizing amidite; screening a functional molecule having affinity for a target substance from the random pool; amplifying the functional molecules having affinity for the target substance, wherein the method further comprises, prior to the amplification step, removing a substituent from the functional molecule having affinity for the target substance.
摘要翻译：提供包含具有引入其碱基的取代基的修饰的核苷酸单元的功能性分子，其中所述取代基可移除地引入所述碱基; 合成酰胺化物的功能性分子，其具有可取代地引入其碱基并用于制备功能分子的取代基; 以及目标物质分析方法，包括：使用合成脒的功能分子制备功能分子的随机库; 从随机池筛选对目标物质具有亲和性的功能分子; 扩增对目标物质具有亲和力的功能分子，其中该方法还包括在扩增步骤之前，从对目标物质具有亲和性的功能分子中除去取代基。

10. 发明申请

US20080167459A1 Amidite for nucleic acid synthesis and nucleic acid synthesizing method 有权
标题翻译：氨脒用于核酸合成和核酸合成方法
公开(公告)号：US20080167459A1
公开(公告)日：2008-07-10
申请号：US11987168
申请日：2007-11-28
申请人： Tsuyoshi Fujihara , Shozo Fujita
发明人： Tsuyoshi Fujihara , Shozo Fujita
IPC分类号： C07H19/02
CPC分类号： C07H19/02 , Y02P20/55
摘要： To provide an amidite for nucleic acid synthesis, which enables a protective group therein to be removed under moderate conditions and can be practically used, and a nucleic acid synthesizing method using the amidite for nucleic acid synthesis. Specifically, the present invention relates to an amidite for nucleic acid synthesis represented by General Formula (I) below, and a nucleic acid synthesizing method using the amidite for nucleic acid synthesis: where X denotes a base; Y denotes a protective group formed of any one of a 4-aminobutyric acid derivative, an o-aminomethylbenzoic acid derivative, an o-aminophenylacetic acid derivative, an o-aminoethylbenzoic acid derivative, an o-aminomethylphenylacetic acid derivative, an o-aminophenylpropionic acid derivative and a 5-aminovaleric acid derivative; and Q denotes one of a hydrogen atom and a hydroxyl group.
摘要翻译：提供用于核酸合成的脒基，其使得其中的保护基能够在中等条件下除去并且可以实际使用，以及使用脒矿进行核酸合成的核酸合成方法。具体而言，本发明涉及下述通式（I）表示的核酸合成用脒盐，以及使用该酰胺化物进行核酸合成的核酸合成方法，其中X表示碱; Y表示由4-氨基丁酸衍生物，邻氨基甲基苯甲酸衍生物，邻氨基苯乙酸衍生物，邻氨基乙基苯甲酸衍生物，邻氨基甲基苯乙酸衍生物，邻氨基苯基丙酸衍生物和5-氨基戊酸衍生物; Q表示氢原子和羟基中的一个。

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