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1. 发明授权

US07264950B1 Reaction system for performing in the amplification of nucleic acids 有权
标题翻译：用于在核酸扩增中进行的反应体系
公开(公告)号：US07264950B1
公开(公告)日：2007-09-04
申请号：US10089498
申请日：2000-09-29
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

2. 发明授权

US06564655B1 Analytical sampling device 失效
标题翻译：分析取样装置
公开(公告)号：US06564655B1
公开(公告)日：2003-05-20
申请号：US10031191
申请日：2002-07-05
申请人： Malcolm Trayton Austen , John Robert Dodgson , Monica Backes , David Wenn , Richard Lynn Hedgeley , Mark Seymour
发明人： Malcolm Trayton Austen , John Robert Dodgson , Monica Backes , David Wenn , Richard Lynn Hedgeley , Mark Seymour
IPC分类号： G01N3510
CPC分类号： G01N1/40 , G01N1/14 , G01N1/405 , G01N33/18 , G01N2001/021
摘要： Apparatus for sampling a liquid, said apparatus comprising: i) a sampling device capable of automatically taking a known volume of liquid from a source; ii) fixing means connected to said sampling device and removably attachable to a solid phase extraction (SPE) unit, the fixing means being arranged such that liquid taken from a sample by the sampling device is passed directly through the solid phase extraction unit; iii) a reading device able to read labels on an SPE unit attached to said fixing means; iv) a controller arranged to log codes based upon a signal from the reading device, and thereafter issue instructions to an operator and/or operate the sampling device to take an appropriate volume of liquid in response to a signal from said reading device. Methods of sampling using the apparatus are also claimed. These are useful in, for example analysis methods.
摘要翻译：用于对液体进行采样的设备，所述设备包括：i）能够自动从源头获取已知体积的液体的采样装置; ii）连接到所述取样装置并可拆卸地连接到固相萃取（SPE）单元的固定装置，所述固定装置被布置成使得通过所述采样装置从样品取出的液体直接通过所述固相提取单元; iii）读取装置，其能够读取附接到所述固定装置的SPE单元上的标签; iv）控制器，基于来自读取装置的信号记录代码，然后向操作者发出指令和/或操作采样装置以响应于来自所述读取装置的信号采取适当体积的液体。使用该装置的采样方法为也声称。这些在例如分析方法中是有用的。

3. 发明授权

US08986927B2 Reaction system for performing in the amplification of nucleic acids 有权
标题翻译：用于在核酸扩增中进行的反应体系
公开(公告)号：US08986927B2
公开(公告)日：2015-03-24
申请号：US12553367
申请日：2009-09-03
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： B01L7/00 , B01L3/00
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

4. 发明授权

US07659096B2 Reaction system for performing in the amplification of nucleic acids 有权
标题翻译：用于在核酸扩增中进行的反应体系
公开(公告)号：US07659096B2
公开(公告)日：2010-02-09
申请号：US11830283
申请日：2007-07-30
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

5. 发明申请

US20080176232A1 Reaction System for Performing in the Amplification of Nucleic Acids 有权
标题翻译：用于核酸扩增的反应体系
公开(公告)号：US20080176232A1
公开(公告)日：2008-07-24
申请号：US11830283
申请日：2007-07-30
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

6. 发明申请

US20090325278A1 Reaction System for Performing in the Amplification of Nucleic Acids 有权
标题翻译：用于核酸扩增的反应体系
公开(公告)号：US20090325278A1
公开(公告)日：2009-12-31
申请号：US12553367
申请日：2009-09-03
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： C12M1/34
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

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