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1. 发明授权

US07264950B1 Reaction system for performing in the amplification of nucleic acids 有权
标题翻译：用于在核酸扩增中进行的反应体系
公开(公告)号：US07264950B1
公开(公告)日：2007-09-04
申请号：US10089498
申请日：2000-09-29
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

2. 发明授权

US08986927B2 Reaction system for performing in the amplification of nucleic acids 有权
标题翻译：用于在核酸扩增中进行的反应体系
公开(公告)号：US08986927B2
公开(公告)日：2015-03-24
申请号：US12553367
申请日：2009-09-03
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： B01L7/00 , B01L3/00
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

3. 发明申请

US20090325278A1 Reaction System for Performing in the Amplification of Nucleic Acids 有权
标题翻译：用于核酸扩增的反应体系
公开(公告)号：US20090325278A1
公开(公告)日：2009-12-31
申请号：US12553367
申请日：2009-09-03
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： C12M1/34
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

4. 发明授权

US07659096B2 Reaction system for performing in the amplification of nucleic acids 有权
标题翻译：用于在核酸扩增中进行的反应体系
公开(公告)号：US07659096B2
公开(公告)日：2010-02-09
申请号：US11830283
申请日：2007-07-30
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

5. 发明申请

US20080176232A1 Reaction System for Performing in the Amplification of Nucleic Acids 有权
标题翻译：用于核酸扩增的反应体系
公开(公告)号：US20080176232A1
公开(公告)日：2008-07-24
申请号：US11830283
申请日：2007-07-30
申请人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
发明人： Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L7/52 , B01L3/52 , B01L3/523
摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
摘要翻译：一种进行扩增反应的方法，所述方法包括向一次性单元（a）中的井提供含有或怀疑含有靶核酸序列的样品（b）实现所述扩增所需的引物，核苷酸和酶反应和（c）缓冲系统，并使该单元经受热循环条件，使得存在于样品中的任何靶核酸被扩增; 其中所述一次性单元包括导热层和面对层，其具有在其间限定的深度达1000微米的一个或多个试剂阱; 并且反应混合物包含以下至少一种：A）其中pH高于8.3的缓冲体系; B）洗涤剂; 和/或C）封端剂。描述了用于实现该方法的装置以及用于该方法的一次性单元。该方法特别适用于快速PCR反应。

6. 发明授权

US08802031B2 Liquid dispensing device with a cap and a diaphragm 有权
标题翻译：具有盖和隔膜的液体分配装置
公开(公告)号：US08802031B2
公开(公告)日：2014-08-12
申请号：US12065659
申请日：2006-09-05
申请人： Martin Alan Lee , David James Squirrell , Georgina Martin
发明人： Martin Alan Lee , David James Squirrell , Georgina Martin
IPC分类号： B01L3/02 , B01L3/00
CPC分类号： B01L3/021 , B01L2400/0481 , G01N35/1002 , G01N2035/103
摘要： A liquid dispensing device comprising a hollow body having an opening in a lower end thereof for receiving liquid, and an integrated cap member arranged to sealingly close the body, said cap member comprising a resilient diaphragm which is deformable in a downwards direction. The device is suitable for use in automated apparatus.
摘要翻译：一种液体分配装置，包括在其下端具有用于接收液体的开口的中空本体，以及设置成密封地封闭所述主体的集成的盖构件，所述盖构件包括可沿向下方向变形的弹性隔膜。该设备适用于自动化设备。

7. 发明申请

US20100297707A1 REACTION VESSEL COMPRISING CONDUCTIVE LAYER AND INNER NON-METALLIC LAYER 有权
标题翻译：包含导电层和内部非金属层的反应容器
公开(公告)号：US20100297707A1
公开(公告)日：2010-11-25
申请号：US12671272
申请日：2008-08-01
申请人： Martin Alan Lee , David James Squirrell , Ross Peter Jones , Roger James Williamson , George Richard Gregory , Graham Gutsell
发明人： Martin Alan Lee , David James Squirrell , Ross Peter Jones , Roger James Williamson , George Richard Gregory , Graham Gutsell
IPC分类号： C12P19/34 , C12M1/00
CPC分类号： B01L7/52 , B01L3/50851 , B01L2300/0858 , B01L2300/0887 , B01L2300/16 , B01L2300/1827 , B01L2300/1883 , G01N21/0332
摘要： A reaction vessel for conducting a chemical or biochemical reaction, such as a polymerase chain reaction wherein at least one wall of said vessel comprises a metallic layer and an inner non-metallic layer. Reaction systems comprising combinations of vessels of the invention and apparatus for heating them, as well as particular reactions vessels are also described and claimed.
摘要翻译：一种用于进行化学或生物化学反应（例如聚合酶链式反应）的反应容器，其中所述容器的至少一个壁包括金属层和内部非金属层。还描述和要求保护包括本发明的容器和用于加热它们的装置的组合的反应系统以及特定的反应容器。

8. 发明授权

US07537927B2 Reaction system for thermal cycling 失效
标题翻译：热循环反应系统
公开(公告)号：US07537927B2
公开(公告)日：2009-05-26
申请号：US11782255
申请日：2007-07-24
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12M1/00 , C12M3/00
CPC分类号： B01L7/52
摘要： Method and apparatus for carrying out a thermal cycling reaction, wherein a succession of samples is conveyed through a series of sequentially arranged temperature control sites, each of the sites comprising means for supplying an electric current to, or inducing an electric current in, sample-containing vessels passing through it so as to induce temperature changes in the samples. Also provided is a sample support and its production, the support comprising a succession of sample vessels arranged sequentially one behind the next, preferably in the form of a linked chain, the support comprising an electrically conducting, preferably plastics, material which heats when an electric current passes through it.
摘要翻译：用于进行热循环反应的方法和装置，其中一系列样品被传送通过一系列顺序排列的温度控制位点，每个位置包括用于向样品中提供电流或引起电流的装置，通过其容纳的容器以引起样品中的温度变化。还提供了一种样品支持体及其生产，该支撑体包括一系列连续的样品容器，这些样品容器顺序地布置在下一个之后，优选地以连接的链条的形式，该支撑体包括导电的，优选塑料的材料，其在电电流通过它。

9. 发明申请

US20080166700A1 Method For Detecting Viable Cells In A Sample By Using A Virus 有权
标题翻译：通过使用病毒检测样品中活细胞的方法
公开(公告)号：US20080166700A1
公开(公告)日：2008-07-10
申请号：US11816331
申请日：2006-02-16
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12Q1/70 , C12N7/00
CPC分类号： C12Q1/689 , C12Q1/70
摘要： A method for detecting viable cells such as bacterial cells, within a sample, said method comprising (i) incubating said sample with a virus which is able to infect said cells under conditions which allow said virus to infect and replicate within any such cells which are viable; (ii) detecting any nucleic acid obtained by replication of the virus in said cell.
摘要翻译：一种用于检测样品内的活细胞如细菌细胞的方法，所述方法包括（i）在允许所述病毒在任何这样的细胞内感染和复制的条件下，使能够感染所述细胞的病毒孵育所述样品，所述病毒是可行的（ii）检测通过在所述细胞中复制病毒获得的任何核酸。

10. 发明授权

US07264961B2 Reaction system for thermal cycling 失效
标题翻译：热循环反应系统
公开(公告)号：US07264961B2
公开(公告)日：2007-09-04
申请号：US10220970
申请日：2001-03-07
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12M1/36
CPC分类号： B01L7/52
摘要： Method and apparatus for carrying out a thermal cycling reaction, wherein a succession of samples is conveyed through a series of sequentially arranged temperature control sites, each of the sites comprising means for supplying an electric current to, or inducing an electric current in, sample-containing vessels passing through it so as to induce temperature changes in the samples. Also provided is a sample support and its production, the support comprising a succession of sample vessels arranged sequentially one behind the next, preferably in the form of a linked chain, the support comprising an electrically conducting, preferably plastics, material which beats when an electric current passes through it.
摘要翻译：用于进行热循环反应的方法和装置，其中一系列样品被传送通过一系列顺序排列的温度控制位点，每个位置包括用于向样品中提供电流或引起电流的装置，通过其容纳的容器以引起样品中的温度变化。还提供了样品支持体及其生产，该支撑体包括一系列连续的样品容器，其顺次地排列在下一个之后，优选地以连接的链条的形式，所述支撑件包括导电的，优选塑料的材料，当电电流通过它。

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