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1. 发明申请

US20070111276A1 Reagent and process for the identification and counting of biological cells 有权
标题翻译：用于生物细胞鉴定和计数的试剂和方法
公开(公告)号：US20070111276A1
公开(公告)日：2007-05-17
申请号：US11647268
申请日：2006-12-29
申请人： Didier Lefevre , Sylvie Veriac , Henri Champseix
发明人： Didier Lefevre , Sylvie Veriac , Henri Champseix
IPC分类号： G01N1/30
CPC分类号： G01N33/5094 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25125 , Y10T436/2525
摘要： The invention relates to a reagent and a process for the identification and counting of biological cells in a sample. This reagent comprises a cell lysing agent selected from at least one detergent in a concentration capable of specifically lysing a given type of cells in the sample, and a stain capable of marking the intracellular nucleic acids of the remaining unlysed cells. Application in particular for the identification and counting of cells using an automated analysis system based on flow cytometry.
摘要翻译：本发明涉及用于鉴定和计数样品中生物细胞的试剂和方法。该试剂包含选自能够特异性裂解样品中给定类型的细胞的浓度的至少一种洗涤剂的细胞裂解剂和能够标记剩余未溶解细胞的细胞内核酸的染色剂。特别适用于使用基于流式细胞术的自动化分析系统鉴定和计数细胞的应用。

2. 发明授权

US5138181A Apparatus for counting and determining at least one leucocytic sub-population 失效
标题翻译：用于计算和确定至少一个白血病分类群体的装置
公开(公告)号：US5138181A
公开(公告)日：1992-08-11
申请号：US602952
申请日：1990-10-26
申请人： Didier Lefevre , Henri Champseix , Serge Champseix
发明人： Didier Lefevre , Henri Champseix , Serge Champseix
IPC分类号： G01N33/49 , G01N15/00 , G01N15/10 , G01N15/12 , G01N15/14
CPC分类号： G01N15/12 , G01N2015/0065 , G01N2015/008 , G01N2015/1037 , G01N2015/1062 , G01N2015/1413
摘要： An apparatus is disclosed for counting and determining at least one leucocytic sub-population, comprising a case for injecting a flow of sample to be analyzed inside a measurement tank through which a light beam passes, comprising internal and external nozzles for sleeving the sample by means of a pressurized liquid. Electrodes for the resistivity measurements of the leucocytic sub-population are component elements of the injection case. Units for collecting and processing the signals delivered by the optical sensor and the electrodes are also provided.
摘要翻译：公开了一种用于计数和确定至少一个白细胞亚群的装置，包括用于将待分析样品流注入光束通过的测量槽内的壳体，其包括用于通过装置套管样品的内部和外部喷嘴的加压液体。白细胞亚群的电阻率测量的电极是注射盒的组成元素。还提供了用于收集和处理由光学传感器和电极传送的信号的单元。

3. 发明申请

US20090059207A1 METHOD AND DEVICE FOR MEASURING PHOTOLUMINESCENCE, ABSORPTION AND DIFFRACTION OF MICROSCOPIC OBJECTS IN A FLUID 审中-公开
标题翻译：用于测量流体中微观对象的光致发光，吸收和衍射的方法和装置
公开(公告)号：US20090059207A1
公开(公告)日：2009-03-05
申请号：US12281906
申请日：2007-03-02
申请人： Philippe Nerin , Benoit Merchez , Jean-Philippe Gineys , Didier Lefevre
发明人： Philippe Nerin , Benoit Merchez , Jean-Philippe Gineys , Didier Lefevre
IPC分类号： G01N21/62
CPC分类号： G01N15/1434 , G01N15/1427 , G01N15/1459 , G01N2015/1486
摘要： The invention relates to a device and to a method for measuring photoluminescence in a fluid present in a measurement vessel. According to the invention, the fluid in the measurement vessel simultaneously receives at least two excitation beams coming from two optical systems. The optical systems are positioned so that their axes form between them a non-zero obtuse angle other than 180° around the measurement vessel. A measurement of light emission is deduced according to the invention from coupling data obtained from emission beams picked up simultaneously by the pickup elements. The optical systems are also positioned in such a manner that there exists at least one partial overlap beam between the excitation beam from the source of a first optical system and the emission beam picked up by the pickup element of a second optical system. The device is also provided with at least one extinction pickup element in the vicinity of at least one of the sources for picking up light at the excitation wavelength in the partial overlap beam, a measurement of absorbance and/or diffraction being deduced from data obtained from the light picked up by the extinction pickup element.
摘要翻译：本发明涉及一种用于测量存在于测量容器中的流体中的光致发光的装置和方法。根据本发明，测量容器中的流体同时接收来自两个光学系统的至少两个激发光束。光学系统被定位成使得它们的轴线在它们之间形成围绕测量容器的180°以外的非零钝角。根据本发明，从由拾取元件同时拾取的发射光束获得的耦合数据推导出发光测量。光学系统也被定位成使得在来自第一光学系统的光源的激发光束和由第二光学系统的拾取元件拾取的发射光束之间存在至少一个部分重叠光束。该装置还在至少一个源附近设置有至少一个消光拾取元件，用于拾取部分重叠光束中的激发波长的光，从根据以下方面获得的数据推导出吸光度和/或衍射的测量值：被灭光元件拾取的光线。

4. 发明授权

US5730941A Device for the optical inspection of a fluid, especially for hematological analyses 失效
标题翻译：用于光学检查流体的装置，特别是用于血液分析
公开(公告)号：US5730941A
公开(公告)日：1998-03-24
申请号：US653325
申请日：1996-05-24
申请人： Didier Lefevre , Bernard Kress
发明人： Didier Lefevre , Bernard Kress
IPC分类号： G01N21/64 , G01N15/14 , G01N21/47 , G01N33/49
CPC分类号： G01N15/14
摘要： It includes a measuring space (8) comprising a constrained passage (15) for the fluid, a monochromatic light source (31) delivering a beam (F1) to optical elements (6, 32, 34) including a first diffractive optical element (32) having a diffractive network (38) predetermined as a function, on the one hand, of the characteristics of the source and, on the other hand, of chosen geometrical and spectral characteristics for the illumination of the constrained passage, and suitable for inducing fluorescence of at least part of the fluid, and first (19-20), second (34-36) and third (40) optoelectronic elements intended to collect, respectively, a transmitted beam, fluorescence and a diffracted beam, and suitable for delivering to an analysis unit (24) signals relating to the contents of the beams. At least one of the beams emanates from a second diffractive optical element (42) having a second diffractive optical network which is predetermined as a function of a frequential and geometrical transfer function of at least one of the optoelectronic elements.
摘要翻译：它包括测量空间（8），其包括用于流体的约束通道（15），将光束（F1）传送到包括第一衍射光学元件（32,32）的光学元件（6,32,34）的单色光源（31））具有作为功能的预定的衍射网络（38），一方面是源的特性，另一方面，对于受限通道的照明的选择的几何和光谱特性，并且适于诱导荧光至少部分流体和第一（19-20），第二（34-36）和第三（40）光电子元件，分别收集透射束，荧光和衍射光束，并且适于传送到分析单元（24）与波束的内容有关的信号。至少一个光束从具有第二衍射光网络的第二衍射光学元件（42）发出，该第二衍射光学网络被预定为至少一个光电元件的频率和几何传递函数的函数。

5. 发明授权

US07777869B2 Device and method for multiparametric analysis of microscopic elements 有权
标题翻译：显微元件多参数分析的装置和方法
公开(公告)号：US07777869B2
公开(公告)日：2010-08-17
申请号：US11912055
申请日：2006-04-14
申请人： Philippe Nerin , Didier Lefevre
发明人： Philippe Nerin , Didier Lefevre
IPC分类号： G01N33/48
CPC分类号： G01N21/6428 , G01N15/1434 , G01N15/147 , G01N2015/1477 , G01N2015/1486 , G01N2015/149 , G01N2015/1493 , G01N2021/6419 , G01N2021/6421
摘要： The invention concerns a device (DA) for analyzing microscopic elements, comprising firstly a measuring space (CM) for microscopic elements to be analyzed, secondly at least one source (S) delivering conjugated rays at the measuring space (CM), having at least two different analyzing wavelengths and designed to interact with the microscopic elements in the measuring space (CM) to form interacting rays, thirdly coding means (M) for encoding the rays upstream of the measuring space (CM) with different codes, fourthly optical filtering means (FO) for selectively filtering the interacting rays of fluorescence and/or diffusion depending on their wavelength, fifthly detecting means (DE, DF) for transforming into electric signals part at least of the interacting rays from the measuring space (CM), and sixthly analyzing means (MA) including decoding means (DRE, DRF) for decoding the electric signals to enable data representing the analyzed microscopic elements to be determined.
摘要翻译：本发明涉及一种用于分析微观元素的装置（DA），其首先包括用于要分析的微观元素的测量空间（CM），其次，在测量空间（CM）处传送共轭射线的至少一个源（S），至少具有两个不同的分析波长，并设计成与测量空间（CM）中的微观元件相互作用以形成相互作用的射线;第三编码装置（M），用于以不同的代码编码测量空间（CM）上游的光线;第四，光学滤波装置（FO），用于根据其波长选择性地过滤荧光和/或扩散的相互作用的光线;第五检测装置（DE，DF），用于将来自测量空间（CM）的至少相互作用的光线部分转换成电信号;以及第六分析装置（MA），包括用于解码电信号的解码装置（DRE，DRF），以使得能够确定表示所分析的微观元素的数据。

6. 发明授权

US07638290B2 Reagent and process for the identification and counting of biological cells 有权
公开(公告)号：US07638290B2
公开(公告)日：2009-12-29
申请号：US11647268
申请日：2006-12-29
申请人： Didier Lefevre , Sylvie Veriac , Henri Champseix
发明人： Didier Lefevre , Sylvie Veriac , Henri Champseix
IPC分类号： G01N33/567
CPC分类号： G01N33/5094 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25125 , Y10T436/2525
摘要： The invention relates to a reagent and a process for the identification and counting of biological cells in a sample. This reagent comprises a cell lysing agent selected from at least one detergent in a concentration capable of specifically lysing a given type of cells in the sample, and a stain capable of marking the intracellular nucleic acids of the remaining unlysed cells. Application in particular for the identification and counting of cells using an automated analysis system based on flow cytometry.

7. 发明申请

US20080124745A1 Method and Device for Characterizing Cellular Components of a Biological Fluid 有权
标题翻译：表征生物流体细胞成分的方法和装置
公开(公告)号：US20080124745A1
公开(公告)日：2008-05-29
申请号：US11572817
申请日：2005-07-06
申请人： Didier Lefevre
发明人： Didier Lefevre
IPC分类号： G01N33/53 , C12Q1/20 , C12M1/34 , G06G7/48
CPC分类号： G01N15/147 , G01N2015/1037 , G01N2015/1477 , Y10T436/10 , Y10T436/101666
摘要： The invention concerns a method for differentiating and counting cellular components present in a biological fluid sample comprising a primary cytological analysis step typically implemented by a flow cytometry equipment (1) to obtain a set of primary results enabling the set of cellular components of the sample to be differentiated and counted in different populations; and a complementary step of cytological analysis of a particular type of cellular components, based on an identified cellular peculiarity, to obtain complementary results enabling at least one cell population or subpopulation of the sample to be differentiated and counted for identification of said cellular peculiarity. The invention is applicable in particular to hematological analyses.
摘要翻译：本发明涉及用于区分和计数存在于生物流体样品中的细胞成分的方法，其包括通常由流式细胞术设备（1）实施的主要细胞学分析步骤，以获得使得该组样品的细胞组分能够在不同的人群中进行区分和计数; 以及基于鉴定的细胞特性的特定类型的细胞组分的细胞学分析的补充步骤，以获得使至少一个细胞群体或样品亚群被分化并计数以鉴定所述细胞特性的互补结果。本发明特别适用于血液学分析。

8. 发明授权

US5232857A Reagent for use in automatic analyzers for distinguisher leukocyte sub-populations in blood samples 失效
标题翻译：在血液样品中用于评估淋巴细胞亚群体的自动分析仪中使用的试剂
公开(公告)号：US5232857A
公开(公告)日：1993-08-03
申请号：US615710
申请日：1990-11-19
申请人： Didier Lefevre , Nadine Voltas , Henri Champseix
发明人： Didier Lefevre , Nadine Voltas , Henri Champseix
IPC分类号： G01N33/49 , C12Q1/06 , G01N33/50
CPC分类号： G01N33/5094 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： The present invention relates to a reagent and to a method of using the same for automatically determining at least one leukocyte sub-population from a total blood sample. Using this reagent, it is possible, in a single step, to lyse the erythrocytes through the action of saponin and SDS, as well as to ensure the differential staining of the leukocyte sub-populations, at the same time preserving the integrity and morphology of the cells, through the combined action of various components including chlorazol black, an alcohol, a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers.
摘要翻译：本发明涉及一种试剂及其使用方法，用于从全血样品中自动测定至少一种白细胞亚种群。使用该试剂，可以在一个步骤中通过皂角苷和SDS的作用裂解红细胞，以及确保白细胞亚群的差异染色，同时保持红细胞的完整性和形态通过各种组分（包括氯唑黑，醇，表面活性剂和防腐剂）的联合作用。根据本发明的试剂可以应用于不同类型的自动流式细胞分析仪。

9. 发明授权

US5196346A Reagent and method of using same for automatically counting basophilic leukocytes in the blood in resistivity variation measuring apparatus 失效
标题翻译：在电阻率变化测量装置中用于自动计算基底荧光素的血液中的试剂和方法
公开(公告)号：US5196346A
公开(公告)日：1993-03-23
申请号：US651564
申请日：1991-02-06
申请人： Didier Lefevre , Henri Champseix
发明人： Didier Lefevre , Henri Champseix
IPC分类号： G01N27/02 , G01N31/22 , G01N33/49 , G01N33/50
CPC分类号： G01N33/5094
摘要： The invention relates to a reagent and a method of using the same for automatically determining the population of the basophilic leukocytes in the blood. The invention relates more particularly to a new formulation for a differential lysing reagent that lyses all the blood cells except for the basophils, particularly suitable for the measuring principle based on resistivity measurement, that is to say the concentrations of the constitutents and the pH of the solution are suitable for ensuring good electrical conductivity of the medium. The reagent according to the invention will find an application in apparatus for automatically determining blood formulae by means of resistivity variation measurements.
摘要翻译：本发明涉及一种试剂及其使用方法，用于自动测定血液中嗜碱性白细胞的总体数。本发明更具体地涉及用于裂解除嗜碱性粒细胞之外的所有血细胞的差异裂解试剂的新制剂，特别适用于基于电阻率测量的测量原理，也就是说组分的浓度和溶液适用于确保介质的良好导电性。根据本发明的试剂将在用于通过电阻率变化测量自动确定血液配方的装置中找到应用。

10. 发明授权

US4520666A Methods and apparatus for determining flow characteristics of a fluid in a well from temperature measurements 失效
标题翻译：用于从温度测量中确定井中流体的流动特性的方法和装置
公开(公告)号：US4520666A
公开(公告)日：1985-06-04
申请号：US560354
申请日：1983-12-12
申请人： Andrea Coblentz , Didier Lefevre , Pascal Colombani , Denis Meunier
发明人： Andrea Coblentz , Didier Lefevre , Pascal Colombani , Denis Meunier
IPC分类号： E21B47/10 , G01F1/68 , G01F1/704 , E21B47/06
CPC分类号： E21B47/1005 , G01F1/68 , G01F1/7044
摘要： The invention relates to method and apparatus for determining the flow rate of fluid in a well over an interval including zones where fluid is exchanged between the well and the formations through which it is bored.A logging sonde is used to measure the temperature of the fluid along said interval, and the flow rate is measured at least at the ends of the interval. Then for each depth, a flow rate value is determined from the measured temperatures and flow rates, and the set of values obtained in this way are recorded.
摘要翻译：本发明涉及用于确定在一个间隔内的井中的流体的流速的方法和装置，包括在井与钻孔之间的地层之间交换流体的区域。测井探头用于沿着所述间隔测量流体的温度，并且至少在间隔的末端测量流速。然后对于每个深度，根据测量的温度和流速确定流量值，并记录以这种方式获得的值集合。

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