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1. 发明授权

US08792162B2 Microscope with illumination switching for capturing sample images during detector integration time 有权
标题翻译：具有照明切换的显微镜，用于在检测器集成时间内捕获样品图像
公开(公告)号：US08792162B2
公开(公告)日：2014-07-29
申请号：US13498078
申请日：2010-09-17
申请人： Helmut Lippert , Ralf Wolleschensky , Robert Hauschild
发明人： Helmut Lippert , Ralf Wolleschensky , Robert Hauschild
IPC分类号： G02B21/06
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/0076
摘要： A microscope including an illumination device for a light sheet having an approximately planar extension along an illumination axis of an illumination beam path with a transverse axis to the illumination axis. Light emitted from the sample region on axis of detection the illumination axis and the axis of detection as well as the transverse axis and the axis of detection being oriented relative at an non-zero angle. The illumination device includes structure deflecting light to different beam path and thus produces an additional sheet of light, together illuminating the sample region on common illumination axis, and with switches between beam paths. Detection device has a detection lens system for light from by the sample region. The switches include a rapidly switching element with a time
摘要翻译：一种显微镜，包括用于光片的照明装置，所述照明装置具有沿着照明轴线的横向轴线的照明光束路径的照明轴线的近似平面的延伸。从检测轴上的样本区域发射的光照射轴和检测轴以及横轴和检测轴相对于非零角度定向。照明装置包括将光转向不同光束路径的结构，从而产生附加的光束，一起在公共照明轴上照射样品区域以及在光束路径之间切换光束。检测装置具有用于来自样品区域的光的检测透镜系统。这些开关包括具有时间<10ms的快速开关元件，表面检测器的预定积分同步使得样品区域在积分期间被照亮两次。

2. 发明授权

US08599480B2 Laser scanning microscope 有权
标题翻译：激光扫描显微镜
公开(公告)号：US08599480B2
公开(公告)日：2013-12-03
申请号：US12691185
申请日：2010-01-21
申请人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
发明人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
IPC分类号： G02B21/06
CPC分类号： G02B21/0032 , G02B21/0048 , G02B21/0076
摘要： A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.
摘要翻译：激光扫描显微镜具有照明光束路径和检测光束路径。提供了一种分束器，其在样品的方向上反射照明光并沿检测装置的方向透过检测光。提供了附加的分光器，用于反射照明光并传输检测光，该附加分光器被布置在照明光束路径中的第一分束器在照明方向下游，并且该附加分束器基本上透射在第一分束器和检测光，但是相对于其反射率获取与第一分束器基本不同的波长范围。

3. 发明授权

US09239454B2 Microscope having light sheet illumination of a sample region 有权
标题翻译：具有样品区域的光照照明的显微镜
公开(公告)号：US09239454B2
公开(公告)日：2016-01-19
申请号：US13498062
申请日：2010-09-17
申请人： Helmut Lippert , Matthias Wald , Michael Goelles , Robert Hauschild , Wolfgang Bathe
发明人： Helmut Lippert , Matthias Wald , Michael Goelles , Robert Hauschild , Wolfgang Bathe
IPC分类号： G01P3/40 , G02B21/00 , G02B21/36 , G02B26/08
CPC分类号： G02B21/0032 , G02B21/0036 , G02B21/006 , G02B21/0064 , G02B21/0076 , G02B21/367 , G02B26/0891
摘要： A microscope including an illumination device providing a light sheet illuminating a sample region, said sheet having a planar extension along an illumination axis of an illumination beam path and a transverse axis lying normal to the illumination axis. A detection device detects light emitted from the sample region on a detection axis the illumination axis and detection axis as well as the transverse axis and the detection axis being oriented relative each other at an angle unequal zero. The detection device has a detection lens system arranged in the detection beam path and splitting means for splitting the detection beam path into two beam sub-paths. A dichroic beam splitter in the infinity region of the surface detectors is about 3 mm thick. Wobble plate(s) disposed orthogonal to each other relative to the detection axis arranged in one of the two beam sub-paths so measured values can be automatically superimposed.
摘要翻译：一种显微镜，包括提供照射样品区域的光片的照明装置，所述片材沿着照明光束路径的照明轴线和垂直于照明轴线的横轴线具有平面延伸。检测装置检测在检测轴上从样本区域发出的光，照明轴和检测轴以及横轴和检测轴相对于彼此以不等于零的角度取向。检测装置具有设置在检测光束路径中的检测透镜系统和用于将检测光束路径分割成两个光束子路径的分离装置。表面检测器的无限区域中的二向色分束器约为3mm厚。摇摆板相对于布置在两个光束子路径之一中的检测轴线彼此正交配置，因此可以自动叠加测量值。

4. 发明申请

US20120281264A1 MICROSCOPE 有权
标题翻译：显微镜
公开(公告)号：US20120281264A1
公开(公告)日：2012-11-08
申请号：US13498095
申请日：2010-09-17
申请人： Helmut Lippert , Matthias Wald , Michael Goelles , Robert Hauschild
发明人： Helmut Lippert , Matthias Wald , Michael Goelles , Robert Hauschild
IPC分类号： G02B21/06 , G02B26/10
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/0076 , G02B21/16 , G02B26/08 , G02B27/0911
摘要： A family of microscopes include an illumination device which produces a planar light sheet along an illumination axis of an illumination beam path and a transverse axis normal to the illumination axis. A detection device detects light emitted from the sample region along an axis of detection of a detection beam path. The illumination and detection axes as well as the transverse axis and the axis of detection being oriented relative each other at an angle unequal to zero. A light sheet generator also produces rotationally symmetrical light and includes structure and control for rapidly scanning the sample region along the transverse axis. The illumination device includes a second light sheet generator having a first astigmatically active optical element with at least one astigmatic lens for producing a static sheet of light. Selection elements used to select either the first or the second light sheet or both together to produce the sheet of light.
摘要翻译：显微镜系列包括照明装置，其沿着照明光束路径的照明轴线和垂直于照明轴线的横轴产生平面光片。检测装置沿着检测光束路径的检测轴检测从样本区域发射的光。照明和检测轴以及横轴和检测轴线以不等于零的角度相对定向。光片发生器还产生旋转对称的光，并且包括用于沿横轴快速扫描样品区域的结构和控制。照明装置包括具有第一散光活性光学元件的第二光片发生器，该光学元件具有用于产生静态光的至少一个散光透镜。用于选择第一或第二光照片或两者的选择元件一起产生光线。

5. 发明申请

US20100188741A1 LASER SCANNING MICROSCOPE 有权
标题翻译：激光扫描显微镜
公开(公告)号：US20100188741A1
公开(公告)日：2010-07-29
申请号：US12691185
申请日：2010-01-21
申请人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
发明人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
IPC分类号： G02B21/06
CPC分类号： G02B21/0032 , G02B21/0048 , G02B21/0076
摘要： A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.
摘要翻译：激光扫描显微镜具有照明光束路径和检测光束路径。提供了一种分束器，其在样品的方向上反射照明光并沿检测装置的方向透过检测光。提供了附加的分光器，用于反射照明光并传输检测光，该附加分光器被布置在照明光束路径中的第一分束器在照明方向下游，并且该附加分束器基本上透射在第一分束器和检测光，但是相对于其反射率获取与第一分束器基本不同的波长范围。

6. 发明授权

US08848268B2 Microscope with light sheet illumination 有权
标题翻译：显微镜带照明灯
公开(公告)号：US08848268B2
公开(公告)日：2014-09-30
申请号：US13498095
申请日：2010-09-17
申请人： Helmut Lippert , Matthias Wald , Michael Goelles , Robert Hauschild
发明人： Helmut Lippert , Matthias Wald , Michael Goelles , Robert Hauschild
IPC分类号： G02B26/08 , G02B21/06 , G02B21/00
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/0076 , G02B21/16 , G02B26/08 , G02B27/0911
摘要： A family of microscopes include an illumination device which produces a planar light sheet along an illumination axis of an illumination beam path and a transverse axis normal to the illumination axis. A detection device detects light emitted from the sample region along an axis of detection of a detection beam path. The illumination and detection axes as well as the transverse axis and the axis of detection being oriented relative each other at an angle unequal to zero. A light sheet generator also produces rotationally symmetrical light and includes structure and control for rapidly scanning the sample region along the transverse axis. The illumination device includes a second light sheet generator having a first astigmatically active optical element with at least one astigmatic lens for producing a static sheet of light. Selection elements used to select either the first or the second light sheet or both together to produce the sheet of light.
摘要翻译：显微镜系列包括照明装置，其沿着照明光束路径的照明轴线和垂直于照明轴线的横轴产生平面光片。检测装置沿着检测光束路径的检测轴检测从样本区域发射的光。照明和检测轴以及横轴和检测轴线以不等于零的角度相对定向。光片发生器还产生旋转对称的光，并且包括用于沿横轴快速扫描样品区域的结构和控制。照明装置包括具有第一散光活性光学元件的第二光片发生器，该光学元件具有用于产生静态光的至少一个散光透镜。用于选择第一或第二光照片或两者的选择元件一起产生光线。

7. 发明申请

US20120200693A1 MICROSCOPE WITH A SHEET OF LIGHT 有权
标题翻译：微光与光照片
公开(公告)号：US20120200693A1
公开(公告)日：2012-08-09
申请号：US13497996
申请日：2010-09-17
申请人： Helmut Lippert , Christopher Power , Robert Hauschild , Matthias Wald
发明人： Helmut Lippert , Christopher Power , Robert Hauschild , Matthias Wald
IPC分类号： H04N7/18
CPC分类号： G02B21/241 , G02B21/002 , G02B21/06
摘要： A family of microscopes with illumination systems directing a sheet of light having an approximately planar extension in an illumination axis of an illumination beam path and in a transverse axis orthogonally oriented to the illumination axis. The microscopes have detection devices used to detect light that is emitted by a sample region. The detection devices including a detection lens system disposed in the detection beam path and an optical detection element spaced from a front lens of the detection lens system and independently adjustable thereof. The optical detection element continuously varies the size of a detection image field and/or continuously displaces a focal plane of detection in the P-region.
摘要翻译：具有照明系统的一系列显微镜，其在照明光束路径的照明轴线和垂直于照明轴线的横向轴线上引导具有近似平面延伸的光线。显微镜具有用于检测由样品区域发射的光的检测装置。检测装置包括设置在检测光束路径中的检测透镜系统和与检测透镜系统的前透镜间隔开的光学检测元件，并且可独立调节。光检测元件连续地改变检测图像场的大小和/或连续地移位P区域中的检测焦平面。

8. 发明授权

US09110301B2 Microscope with a sheet of light 有权
标题翻译：显微镜带一张光
公开(公告)号：US09110301B2
公开(公告)日：2015-08-18
申请号：US13497996
申请日：2010-09-17
申请人： Helmut Lippert , Christopher Power , Robert Hauschild , Matthias Wald
发明人： Helmut Lippert , Christopher Power , Robert Hauschild , Matthias Wald
IPC分类号： G02B21/06 , G02B21/24 , G02B21/00
CPC分类号： G02B21/241 , G02B21/002 , G02B21/06
摘要： A family of microscopes with illumination systems directing a sheet of light having an approximately planar extension in an illumination axis of an illumination beam path and in a transverse axis orthogonally oriented to the illumination axis. The microscopes have detection devices used to detect light that is emitted by a sample region. The detection devices including a detection lens system disposed in the detection beam path and an optical detection element spaced from a front lens of the detection lens system and independently adjustable thereof. The optical detection element continuously varies the size of a detection image field and/or continuously displaces a focal plane of detection in the P-region.
摘要翻译：具有照明系统的一系列显微镜，其在照明光束路径的照明轴线和垂直于照明轴线的横向轴线上引导具有近似平面延伸的光线。显微镜具有用于检测由样品区域发射的光的检测装置。检测装置包括设置在检测光束路径中的检测透镜系统和与检测透镜系统的前透镜间隔开的光学检测元件，并且可独立调节。光检测元件连续地改变检测图像场的大小和/或连续地移位P区域中的检测焦平面。

9. 发明申请

US20120176674A1 MICROSCOPE 有权
标题翻译：显微镜
公开(公告)号：US20120176674A1
公开(公告)日：2012-07-12
申请号：US13498078
申请日：2010-09-17
申请人： Helmut Lippert , Ralf Wolleschensky , Robert Hauschild
发明人： Helmut Lippert , Ralf Wolleschensky , Robert Hauschild
IPC分类号： G02B21/06
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/0076
摘要： A microscope including an illumination device for a light sheet having an approximately planar extension along an illumination axis of an illumination beam path with a transverse axis to the illumination axis. Light emitted from the sample region on axis of detection the illumination axis and the axis of detection as well as the transverse axis and the axis of detection being oriented relative at an non-zero angle. The illumination device includes structure deflecting light to different beam path and thus produces an additional sheet of light, together illuminating the sample region on common illumination axis, and with switches between beam paths. Detection device has a detection lens system for light from by the sample region. The switches include a rapidly switching element with a time
摘要翻译：一种显微镜，包括用于光片的照明装置，所述照明装置具有沿着照明轴线的横向轴线的照明光束路径的照明轴线的近似平面的延伸。从检测轴上的样本区域发射的光照射轴和检测轴以及横轴和检测轴相对于非零角度定向。照明装置包括将光转向不同光束路径的结构，从而产生附加的光束，一起在公共照明轴上照射样品区域以及在光束路径之间切换光束。检测装置具有用于来自样品区域的光的检测透镜系统。这些开关包括具有时间<10ms的快速开关元件，表面检测器的预定积分同步使得样品区域在积分期间被照亮两次。

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