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1. 发明申请

US20120176674A1 MICROSCOPE 有权
标题翻译：显微镜
公开(公告)号：US20120176674A1
公开(公告)日：2012-07-12
申请号：US13498078
申请日：2010-09-17
申请人： Helmut Lippert , Ralf Wolleschensky , Robert Hauschild
发明人： Helmut Lippert , Ralf Wolleschensky , Robert Hauschild
IPC分类号： G02B21/06
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/0076
摘要： A microscope including an illumination device for a light sheet having an approximately planar extension along an illumination axis of an illumination beam path with a transverse axis to the illumination axis. Light emitted from the sample region on axis of detection the illumination axis and the axis of detection as well as the transverse axis and the axis of detection being oriented relative at an non-zero angle. The illumination device includes structure deflecting light to different beam path and thus produces an additional sheet of light, together illuminating the sample region on common illumination axis, and with switches between beam paths. Detection device has a detection lens system for light from by the sample region. The switches include a rapidly switching element with a time
摘要翻译：一种显微镜，包括用于光片的照明装置，所述照明装置具有沿着照明轴线的横向轴线的照明光束路径的照明轴线的近似平面的延伸。从检测轴上的样本区域发射的光照射轴和检测轴以及横轴和检测轴相对于非零角度定向。照明装置包括将光转向不同光束路径的结构，从而产生附加的光束，一起在公共照明轴上照射样品区域以及在光束路径之间切换光束。检测装置具有用于来自样品区域的光的检测透镜系统。这些开关包括具有时间<10ms的快速开关元件，表面检测器的预定积分同步使得样品区域在积分期间被照亮两次。

2. 发明授权

US08792162B2 Microscope with illumination switching for capturing sample images during detector integration time 有权
标题翻译：具有照明切换的显微镜，用于在检测器集成时间内捕获样品图像
公开(公告)号：US08792162B2
公开(公告)日：2014-07-29
申请号：US13498078
申请日：2010-09-17
申请人： Helmut Lippert , Ralf Wolleschensky , Robert Hauschild
发明人： Helmut Lippert , Ralf Wolleschensky , Robert Hauschild
IPC分类号： G02B21/06
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/0076
摘要： A microscope including an illumination device for a light sheet having an approximately planar extension along an illumination axis of an illumination beam path with a transverse axis to the illumination axis. Light emitted from the sample region on axis of detection the illumination axis and the axis of detection as well as the transverse axis and the axis of detection being oriented relative at an non-zero angle. The illumination device includes structure deflecting light to different beam path and thus produces an additional sheet of light, together illuminating the sample region on common illumination axis, and with switches between beam paths. Detection device has a detection lens system for light from by the sample region. The switches include a rapidly switching element with a time
摘要翻译：一种显微镜，包括用于光片的照明装置，所述照明装置具有沿着照明轴线的横向轴线的照明光束路径的照明轴线的近似平面的延伸。从检测轴上的样本区域发射的光照射轴和检测轴以及横轴和检测轴相对于非零角度定向。照明装置包括将光转向不同光束路径的结构，从而产生附加的光束，一起在公共照明轴上照射样品区域以及在光束路径之间切换光束。检测装置具有用于来自样品区域的光的检测透镜系统。这些开关包括具有时间<10ms的快速开关元件，表面检测器的预定积分同步使得样品区域在积分期间被照亮两次。

3. 发明申请

US20110036996A1 APPARATUS AND METHOD FOR HIGH SPATIAL RESOLUTION IMAGING OF A STRUCTURE OF A SAMPLE 有权
标题翻译：用于样品结构的高空间分辨率成像的装置和方法
公开(公告)号：US20110036996A1
公开(公告)日：2011-02-17
申请号：US12867291
申请日：2009-02-03
申请人： Ralf Wolleschensky , Helmut Lippert , Christopher Power , Benno Radt
发明人： Ralf Wolleschensky , Helmut Lippert , Christopher Power , Benno Radt
IPC分类号： G01J1/58
CPC分类号： G02B21/002 , G01N21/6458 , G02B21/10 , G02B27/58
摘要： Apparatus and method for high spatial resolution imaging of a sample's structure, including a diffraction-limited resolution volume with a plurality of dye molecules which can be switched between different states and have a distribution density which is greater than the inverse of the diffraction-limited resolution volume, where at least one state is fluorescing, the fluorescence being collected by an objective lens and imaged on a spatially resolving detector by an optical system. At least one light source provided for emitting a switching radiation and for emitting an excitation radiation. At least one of the light sources is arranged to radiate through the sample, and a switching and/or fluorescence excitation of the dye molecules is carried out. The switching is a photoactivation or a photodeactivation of the dye molecules. A focusing arrangement is provided for switching and/or for excitation to generate a line-like illumination region extending in a direction of illumination.
摘要翻译：用于样品结构的高空间分辨率成像的装置和方法，包括具有多个染料分子的衍射限制分辨率体积，其可以在不同状态之间切换并且具有大于衍射受限分辨率的反比的分布密度体积，其中至少一个状态是荧光的，荧光由物镜收集并通过光学系统在空间分辨检测器上成像。提供用于发射切换辐射并用于发射激发辐射的至少一个光源。至少一个光源布置成通过样品辐射，并且进行染料分子的切换和/或荧光激发。切换是染料分子的光活化或光激活。提供了用于切换和/或激发的聚焦装置，以产生在照明方向上延伸的线状照明区域。

4. 发明授权

US08362448B2 Apparatus and method for high spatial resolution imaging of a structure of a sample 有权
标题翻译：用于样品结构的高空间分辨率成像的装置和方法
公开(公告)号：US08362448B2
公开(公告)日：2013-01-29
申请号：US12867291
申请日：2009-02-03
申请人： Ralf Wolleschensky , Helmut Lippert , Christopher Power , Benno Radt
发明人： Ralf Wolleschensky , Helmut Lippert , Christopher Power , Benno Radt
IPC分类号： G01J1/58
CPC分类号： G02B21/002 , G01N21/6458 , G02B21/10 , G02B27/58
摘要： Apparatus and method for high spatial resolution imaging of a sample's structure, including a diffraction-limited resolution volume with a plurality of dye molecules which can be switched between different states and have a distribution density which is greater than the inverse of the diffraction-limited resolution volume, where at least one state is fluorescing, the fluorescence being collected by an objective lens and imaged on a spatially resolving detector by an optical system. At least one light source provided for emitting a switching radiation and for emitting an excitation radiation. At least one of the light sources is arranged to radiate through the sample, and a switching and/or fluorescence excitation of the dye molecules is carried out. The switching is a photoactivation or a photodeactivation of the dye molecules. A focusing arrangement is provided for switching and/or for excitation to generate a line-like illumination region extending in a direction of illumination.
摘要翻译：用于样品结构的高空间分辨率成像的装置和方法，包括具有多个染料分子的衍射限制分辨率体积，其可以在不同状态之间切换并且具有大于衍射受限分辨率的反比的分布密度体积，其中至少一个状态是荧光的，荧光由物镜收集并通过光学系统在空间分辨检测器上成像。提供用于发射切换辐射并用于发射激发辐射的至少一个光源。至少一个光源布置成通过样品辐射，并且进行染料分子的切换和/或荧光激发。切换是染料分子的光活化或光激活。提供了用于切换和/或激发的聚焦装置，以产生在照明方向上延伸的线状照明区域。

5. 发明申请

US20100265575A1 MICROSCOPE 审中-公开
标题翻译：显微镜
公开(公告)号：US20100265575A1
公开(公告)日：2010-10-21
申请号：US12809136
申请日：2008-12-10
申请人： Helmut Lippert , Ralf Wolleschensky
发明人： Helmut Lippert , Ralf Wolleschensky
IPC分类号： G02B21/06
CPC分类号： G02B21/06 , G02B21/10 , G02B21/16 , G02B26/0816 , G02B27/0911 , G02B27/58
摘要： A microscope including an imaging objective for imaging a sample on a detector and means for illuminating the sample with a light sheet in the focus plane of the imaging objective. The illumination means includes an illumination source which emits coherent light, and Bessel optics which generate at least two plane waves from the light beam and give propagation directions for the plane waves. The propagation direction of each of the plane waves encloses an acute angle with the focus plane in each instance, the magnitude of the acute angle being identical for each of the plane waves, so that the plane waves undergo constructive interference in the focus plane so that a light sheet is generated. Similarly, the illumination means can also include an optical element by which a rotationally symmetric Bessel beam is generated from the light beam for dynamic generation of a light sheet.
摘要翻译：一种显微镜，包括用于在检测器上成像样品的成像目标和用于在成像物镜的聚焦平面中用光照射样品的装置。照明装置包括发射相干光的照明源和从光束产生至少两个平面波并给出平面波的传播方向的贝塞尔光学器件。每个平面波的传播方向在每种情况下都与焦点平面成锐角，对于每个平面波，锐角的大小相同，使得平面波在聚焦平面中经历相长的干涉，使得产生光照片。类似地，照明装置还可以包括光学元件，通过该光学元件从光束产生旋转对称的贝塞尔光束用于光片的动态生成。

6. 发明申请

US20100188741A1 LASER SCANNING MICROSCOPE 有权
标题翻译：激光扫描显微镜
公开(公告)号：US20100188741A1
公开(公告)日：2010-07-29
申请号：US12691185
申请日：2010-01-21
申请人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
发明人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
IPC分类号： G02B21/06
CPC分类号： G02B21/0032 , G02B21/0048 , G02B21/0076
摘要： A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.
摘要翻译：激光扫描显微镜具有照明光束路径和检测光束路径。提供了一种分束器，其在样品的方向上反射照明光并沿检测装置的方向透过检测光。提供了附加的分光器，用于反射照明光并传输检测光，该附加分光器被布置在照明光束路径中的第一分束器在照明方向下游，并且该附加分束器基本上透射在第一分束器和检测光，但是相对于其反射率获取与第一分束器基本不同的波长范围。

7. 发明授权

US08599480B2 Laser scanning microscope 有权
标题翻译：激光扫描显微镜
公开(公告)号：US08599480B2
公开(公告)日：2013-12-03
申请号：US12691185
申请日：2010-01-21
申请人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
发明人： Matthias Danckwerts , Ralf Wolleschensky , Joerg Steinert , Robert Hauschild , Stefan Wilhelm
IPC分类号： G02B21/06
CPC分类号： G02B21/0032 , G02B21/0048 , G02B21/0076
摘要： A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.
摘要翻译：激光扫描显微镜具有照明光束路径和检测光束路径。提供了一种分束器，其在样品的方向上反射照明光并沿检测装置的方向透过检测光。提供了附加的分光器，用于反射照明光并传输检测光，该附加分光器被布置在照明光束路径中的第一分束器在照明方向下游，并且该附加分束器基本上透射在第一分束器和检测光，但是相对于其反射率获取与第一分束器基本不同的波长范围。

8. 发明授权

US09588328B2 Wide-field microscope and method for wide-field microscopy 有权
标题翻译：宽视场显微镜和广视野显微镜的方法
公开(公告)号：US09588328B2
公开(公告)日：2017-03-07
申请号：US14112519
申请日：2012-03-05
申请人： Thomas Kalkbrenner , Ralf Wolleschensky , Ingo Kleppe
发明人： Thomas Kalkbrenner , Ralf Wolleschensky , Ingo Kleppe
IPC分类号： G02B21/36 , G01N21/64 , G02B21/16 , G02B27/58 , H04N5/372
CPC分类号： G02B21/365 , G01N21/6408 , G01N21/6458 , G02B21/16 , G02B21/361 , G02B21/367 , G02B27/58 , H04N5/372
摘要： A method for high-resolution PAL microscopy, wherein a sample field is imaged on a detector surface of a detector, the sample field is imaged into an image field which is smaller than the detector surface, and the image field on the detector surface is shifted, so that the same sample field is imaged in different positions located adjacent to one another on the image field in order to determine information about changes in the sample field.
摘要翻译：一种用于高分辨率PAL显微镜的方法，其中将样品场成像在检测器的检测器表面上，将样本场成像为小于检测器表面的图像场，并且检测器表面上的图像场偏移，使得相同的采样场成像在图像场上彼此相邻的不同位置，以便确定关于样本场中的变化的信息。

9. 发明申请

US20140133011A1 CONFOCAL INCIDENT-LIGHT SCANNING MICROSCOPE 有权
标题翻译：共焦事件光扫描显微镜
公开(公告)号：US20140133011A1
公开(公告)日：2014-05-15
申请号：US14130966
申请日：2012-07-05
申请人： Daniel Schwedt , Ralf Wolleschensky
发明人： Daniel Schwedt , Ralf Wolleschensky
IPC分类号： G02B21/00 , G02F1/29 , G02F1/35
CPC分类号： G02B21/0076 , G02B21/0032 , G02F1/29 , G02F1/353
摘要： A microscope which makes possible a spectrally-flexible excitation and detection of fluorescence in an economical manner. For this purpose, means for frequency conversion are arranged in the common beam path and a filter for excitation light is arranged in addition to the main beam splitter in the detection beam path. The frequency conversion achieves a spectral delimitation between illumination light, which is emitted by the light source, and excitation light which brings about fluorescence excitation in the specimen. Because the frequency conversion takes place in the common beam path after the main beam splitter, it is possible for both a spatial separation of illumination light, and excitation light and fluorescent light (detection light) emitted by the specimen, to be carried out in an economical manner at the main beamsplitter according to spectral bands because of the spectral difference between illumination light and excitation light.
摘要翻译：一种显微镜，可以以经济的方式进行光谱灵活的荧光激发和检测。为此，在公共光束路径中布置用于频率转换的装置，并且除了检测光束路径中的主分束器之外还布置有用于激发光的滤光器。频率转换实现了由光源发射的照明光与在样本中产生荧光激发的激发光之间的光谱定界。由于在主分束器之后的共同光束路径中发生频率转换，所以可以在照射光和激发光的空间分离以及由样本发射的荧光（检测光）两者中进行由于照明光和激发光之间的光谱差异，根据光谱带在主分束器处经济。

10. 发明授权

US08699130B2 Microscope, in particular laser scanning microscope 有权
标题翻译：显微镜，特别是激光扫描显微镜
公开(公告)号：US08699130B2
公开(公告)日：2014-04-15
申请号：US13255627
申请日：2010-03-06
申请人： Ralf Wolleschensky
发明人： Ralf Wolleschensky
IPC分类号： G02B21/36 , G02B21/00
CPC分类号： G02B21/0076 , G01J3/02 , G01J3/0237 , G01J3/26 , G01J3/36 , G01J3/4406 , G01J2003/1221 , G01N21/645 , G01N2021/6471 , G02B21/0064 , G02B27/1006
摘要： Microscope, particularly laser scanning microscope, for optical detection of light radiation excited in a specimen, having a detection beam path for detecting spectral components of the light radiation in a plurality of detection channels, wherein the light radiation arrives at a variable longpass filter or shortpass filter from which reflected and/or transmitted components are reflected back with a parallel offset, and the latter arrive at a detector after at least one back-reflection of this kind.
摘要翻译：显微镜，特别是激光扫描显微镜，用于在样本中激发的光辐射的光学检测，具有用于检测多个检测通道中的光辐射的光谱分量的检测光束路径，其中光辐射到达可变长通滤波器或短路反射和/或透射的分量由平行偏移反射回来的滤光器，后者在这种至少一次背反射之后到达检测器。

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