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1. 发明申请

US20110097706A1 MICRO-REACTOR FOR OBSERVING PARTICLES IN A FLUID 有权
标题翻译：用于在流体中观察颗粒的微反应器
公开(公告)号：US20110097706A1
公开(公告)日：2011-04-28
申请号：US12912468
申请日：2010-10-26
申请人： Gerard Anne Nicolaas van Veen , Jacobus Peter Johannes Peters , Pleun Dona , Alan Frank De Jong
发明人： Gerard Anne Nicolaas van Veen , Jacobus Peter Johannes Peters , Pleun Dona , Alan Frank De Jong
IPC分类号： C12Q1/70 , G01N15/00 , G01N21/00 , C12M1/34 , G01N33/53 , G01N23/00 , G01N21/76 , G01N33/554 , G01N31/10
CPC分类号： B01J19/0093 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/0086 , B01J2219/00905 , B01J2219/0097 , B01L3/502761 , B01L7/54 , B01L2200/0668 , B01L2300/0822 , B01L2400/0415 , B01L2400/0439 , B01L2400/0442 , B01L2400/0487 , G01N2021/058 , H01J37/20 , H01J37/26 , H01J2237/2004 , Y10T436/143333
摘要： The invention relates to a micro-reactor for observing small particles, cells, bacteria, viruses or protein molecules in a fluid. The micro-reactor shows a first channel formed between two layers for containing the fluid, with an inlet and an outlet, the two layers separated by a first distance. A likewise second channel with an inlet and an outlet is placed adjacent to the first channel. A gap connects the first channel and the second channel, at the gap at least one layer showing a window transparent to the method of inspection and at the window the two layers being separated by a very small distance of, for example, 1 μm or less.The micro-reactor may be used with an optical microscope (in which all particles are in focus), inspection with a Scanning Transmission Electron Microscope (in which the range of the electrons is limited), inspection with soft X-rays in the 250-500 eV range (also showing a limited range), etc. A method of using the micro-reactor includes applying a gradient over the gap, thereby causing the particles to cross the gap. The gradient may be static or dynamic, and may be a gradient in concentration, of a chemical or biological material, in pressure, in temperature, in electric potential, or in magnetic field. By detecting a property of the particles upstream in the first channel, e.g. using fluorescent labels on the particles, and then applying a pressure burst over the channels when the property meets certain preset criteria, only selected particles can be placed in the gap.
摘要翻译：本发明涉及用于观察流体中的小颗粒，细胞，细菌，病毒或蛋白质分子的微反应器。微反应器示出了在两层之间形成的用于容纳流体的第一通道，具有入口和出口，两层分开第一距离。具有入口和出口的同样的第二通道被放置成与第一通道相邻。间隙连接第一通道和第二通道，在间隙处，至少一层显示对于检查方法是透明的窗口，并且在窗口处，两层被隔开非常小的距离，例如1μm或更小。微反应器可以与光学显微镜一起使用（其中所有颗粒都在焦点中），用扫描透射电子显微镜（其中电子的范围被限制）进行检查，在250- 500eV范围（也显示有限范围）等。使用微反应器的方法包括在间隙上施加梯度，从而使颗粒穿过间隙。梯度可以是静态或动态的，并且可以是化学或生物材料在压力，温度，电位或磁场中的浓度梯度。通过检测第一通道上游的颗粒的性质，例如，在颗粒上使用荧光标记，然后当性能达到某些预设标准时，在通道上施加压力突发，只有选定的颗粒才能放置在间隙中。

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