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1. 发明授权

US10066251B2 Polypeptides having catalase activity and polynucleotides encoding same 有权
公开(公告)号：US10066251B2
公开(公告)日：2018-09-04
申请号：US15466709
申请日：2017-03-22
申请人： Novozymes Inc.
发明人： Ye Liu , Junxin Duan , Yu Zhang , Lan Tang
IPC分类号： C12S3/00 , C12S3/02 , C12S3/04 , C12P1/00 , C12P19/02 , C12N9/08
摘要： The present invention relates to isolated polypeptides having catalase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

2. 发明授权

US08372609B2 Process for producing a sugar solution by combined chemical and enzymatic saccharification of polysaccharide enriched biomass 有权
标题翻译：通过多糖富集生物质的化学和酶促糖化生产糖溶液的方法
公开(公告)号：US08372609B2
公开(公告)日：2013-02-12
申请号：US12621585
申请日：2009-11-19
申请人： Subramaniam Sabesan
发明人： Subramaniam Sabesan
IPC分类号： C08B37/00 , C07H1/00 , C07H1/06 , C07H1/08 , C07H3/00 , C13K5/00 , C13K7/00 , C12P1/00 , C12P7/00 , C12P19/00 , C12P19/04 , C12S3/02 , C12S3/04
CPC分类号： D21C3/003 , C08H6/00 , C08H8/00 , C12N9/2437 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , C13K1/02 , D21C3/02 , D21C5/005 , Y02E50/16
摘要： Concentrated sugar solutions obtained from polysaccharide enriched biomass by contacting biomass with water and at least one nucleophilic base to produce a polysaccharide enriched biomass comprising a solid fraction and a liquid fraction and then contacting enriched biomass with a dilute mineral acid selected from the group consisting of sulfuric acid, phosphoric acid, hydrochloric acid, nitric acid, or a combination thereof, to produce an intermediate saccharification product, which is contacted with an enzyme consortium to produce a final saccharification product comprising fermentable sugars.
摘要翻译：通过将生物质与水和至少一个亲核碱接触以产生富含多糖的生物质，从富含多糖的生物质获得的浓缩糖溶液，其包含固体部分和液体部分，然后使富含的生物质与选自硫酸酸，磷酸，盐酸，硝酸或其组合，以产生中间糖化产物，其与酶联接体接触以产生包含可发酵糖的最终糖化产物。

3. 发明授权

US5518921A Biological control for wood products and debarking 失效
标题翻译：木制品的生物防治和剥皮
公开(公告)号：US5518921A
公开(公告)日：1996-05-21
申请号：US424134
申请日：1995-04-17
申请人： Robert A. Blanchette , Roberta L. Farrell , Chad J. Behrendt
发明人： Robert A. Blanchette , Roberta L. Farrell , Chad J. Behrendt
IPC分类号： B27L1/00 , B27K5/00 , B27K5/02 , B27L3/00 , C12S3/00 , C12S3/04 , C12S11/00
CPC分类号： B27K3/002 , B27K3/0207
摘要： Fungi which grow white/colorless and reduce pitch are used to protect structural wood before or after cutting from logs against color staining by staining fungi. The fungus Phanerochaete gigantea is also useful to facilitate debarking.
摘要翻译：生长白色/无色和减少沥青的真菌用于在从木材切割之前或之后保护结构木材，通过染色真菌来染色。真菌Phanerochaete gigantea也有助于剥皮。

4. 发明授权

US5476789A Composition containing strains of Ophiostoma piliferum and a method of using the composition to reduce the pitch content of pulpwood or pulp 失效
标题翻译：含有Ophiostoma piliferum菌株的组合物和使用该组合物降低纸浆木浆或纸浆沥青含量的方法
公开(公告)号：US5476789A
公开(公告)日：1995-12-19
申请号：US131260
申请日：1993-10-01
申请人： Roberta L. Farrell , Yitzhak Hadar , Philip A. Wendler , Wendy Zimmerman
发明人： Roberta L. Farrell , Yitzhak Hadar , Philip A. Wendler , Wendy Zimmerman
IPC分类号： C12N1/14 , C12R1/645 , C12S3/04 , C12S3/08 , D21B1/02 , D21C1/00 , D21C5/00 , D21C9/08 , A01N63/00 , C07G17/00 , D21C3/00 , D21C3/20
CPC分类号： C12R1/645 , C12R1/77 , D21C5/005 , D21C9/08 , Y10S162/04 , Y10S162/12 , Y10S435/911
摘要： Ascospores of wood-penetrating, pitch-grading fungi of the class of Ascomycotina and Deuteromycotina, e.g. Ophiostromas, may be screened to provide fungi combining the properties of good growth on non-sterile wood substrates and minimized or even enhanced brightness effects for use in pitch reduction of wood substrates, e.g. logs and wood chips. A new and improved method of isolating such ascospores involving effective suspension in an oil consumable by the fungus, e.g. a vegetable oil, and then treatment of the oil with a dispersing agent is also disclosed.
摘要翻译：子囊菌属和二月子霉菌类的木材穿透，沥青分级真菌的孢子，例如可以筛选Ophiostromas以提供真菌，其结合在非无菌木质基材上良好生长的性质，并且最小化甚至增强的亮度效果用于木质底物的沥青减少，例如。原木和木片。一种分离这种子囊孢子的新的和改进的方法，其涉及由真菌的油消耗品中的有效悬浮液，例如，植物油，然后用分散剂处理油。

5. 发明授权

US5466601A Selectively removing embedded lint precursors with cellulase 失效
标题翻译：用纤维素酶选择性去除嵌入的棉绒前体
公开(公告)号：US5466601A
公开(公告)日：1995-11-14
申请号：US188051
申请日：1994-01-26
申请人： Terry L. Jenkins , Michael L. McAbee , Joe W. Richardson, Jr.
发明人： Terry L. Jenkins , Michael L. McAbee , Joe W. Richardson, Jr.
IPC分类号： C12P19/14 , C12S3/04 , C12S11/00 , D06M16/00 , C09B67/00 , D01B1/04
CPC分类号： D06M16/003 , C12P19/14
摘要： The present invention comprises a three step process that selectively removes embedded cellulose lint precursors from a cotton fabric with a cellulase solution during a continuous fabric manufacturing process. The cellulase solution, applied continually, travels in a finite, limited path and digests fibrils, forming embedded lint precursors, from the fabric. Undigestable fibrils, located outside the path travelled by the cellulase, remain undamaged by the cellulase. After the fabric's first wash, no appreciable linting is observed on the surface thereof and the fabric retains at least about 90% of its strength. Further, the process produces fabrics which do not exhibit commercially unacceptable yellowing from the action of the cellulase upon the cellulose.
摘要翻译：本发明包括三步法，其在连续的织物制造过程中用纤维素酶溶液从棉织物中选择性去除嵌入的纤维素棉绒前体。不断应用的纤维素酶溶液在有限的途径中行进，从织物中消化原纤维，形成嵌入的棉绒前体。位于纤维素酶途径之外的不可消化的原纤维仍然被纤维素酶损伤。在织物的第一次洗涤之后，在其表面上没有观察到明显的棉绒，并且织物保持其强度的至少约90％。此外，该方法产生织物，其从纤维素酶对纤维素的作用不显示商业上不可接受的黄变。

6. 发明授权

US08383888B1 Plants and plant products useful for biofuel manufacture and feedstock, and methods of producing same 有权
标题翻译：用于生物燃料生产和原料的植物和植物产品及其生产方法
公开(公告)号：US08383888B1
公开(公告)日：2013-02-26
申请号：US12560594
申请日：2009-09-16
申请人： Seth DeBolt , Darby Harris , Jozsef Stork
发明人： Seth DeBolt , Darby Harris , Jozsef Stork
IPC分类号： C12S3/04 , C12P39/00
CPC分类号： C12P19/14 , C12N9/1059 , C12N15/8245 , C12N15/8246 , C12P7/10 , C12P7/14 , Y02E50/16 , Y02E50/343
摘要： A method of processing plant cellulose includes providing plant cellulose that is from a plant expressing a CESA polypeptide variant having at least one amino acid mutation in its carboxy-terminal transmembrane region; and saccharifying the plant cellulose to produce fermentable sugars. The method can also include fermenting the fermentable sugars to produce alcohol. A method of producing a plant having beneficial saccharification properties includes introducing into a plant a polynucleotide encoding a CESA polypeptide variant having at least one amino acid mutation in its carboxy-terminal transmembrane region; and expressing in the plant the CESA polypeptide variant, wherein plant cellulose of the plant expressing the CESA polypeptide variant has beneficial saccharification properties as compared to a wild-type plant.
摘要翻译：处理植物纤维素的方法包括提供来自表达在其羧基末端跨膜区具有至少一个氨基酸突变的CESA多肽变体的植物的植物纤维素; 并将植物纤维素糖化以产生可发酵糖。该方法还可以包括发酵可发酵糖以产生醇。制备具有有利糖化特性的植物的方法包括将编码在其羧基末端跨膜区具有至少一个氨基酸突变的CESA多肽变体的多核苷酸引入植物; 并且在植物中表达CESA多肽变体，其中表达CESA多肽变体的植物的植物纤维素与野生型植物相比具有有利的糖化特性。

7. 发明申请

US20090098638A1 INCREASED FIBER HYDROLYSIS BY PROTEASE ADDITION 有权
标题翻译：通过蛋白质添加剂增加纤维水解
公开(公告)号：US20090098638A1
公开(公告)日：2009-04-16
申请号：US12247499
申请日：2008-10-08
申请人： Charles A. Abbas , Wu-Li Bao
发明人： Charles A. Abbas , Wu-Li Bao
IPC分类号： C12S3/04 , C12S3/12
CPC分类号： C12P19/02 , C12P19/20 , Y02E50/16
摘要： Novel fiber processing methods and the products obtained therefrom are disclosed. Methods may include thermochemical and/or enzymatic hydrolysis of fiber feedstocks including distillers' dried grains, distillers' dried grains with solubles, soyhull, miscanthus and switchgrass. Enzymatic hydrolysis includes hydrolysis with cellulase, hemicellulase, and protease.
摘要翻译：公开了新颖的纤维加工方法及其产品。方法可以包括纤维原料的热化学和/或酶水解，包括蒸馏器的干燥谷物，具有可溶物的蒸馏器干燥谷物，豆油，芒草和柳枝稷。酶水解包括用纤维素酶，半纤维素酶和蛋白酶水解。

8. 发明申请

US20050070003A1 Endoglucanases 有权
标题翻译：内切葡聚糖酶
公开(公告)号：US20050070003A1
公开(公告)日：2005-03-31
申请号：US10965499
申请日：2004-10-14
申请人： Martin Schulein , Torben Henriksen , Lene Andersen , Soren Lassen , Markus Kauppinen , Lene Lange , Ruby Nielsen , Shinobu Takagi , Michiko Ihara
发明人： Martin Schulein , Torben Henriksen , Lene Andersen , Soren Lassen , Markus Kauppinen , Lene Lange , Ruby Nielsen , Shinobu Takagi , Michiko Ihara
IPC分类号： C12N15/09 , C05F11/08 , C05F17/00 , C07G99/00 , C11D3/386 , C12N1/19 , C12N9/42 , C12N15/00 , C12R1/645 , C12R1/69 , C12R1/77 , C12R1/80 , C12S3/04 , C12S11/00 , D06M13/02 , D06M13/322 , D06M13/46 , D06M16/00 , D06P5/02 , D06P5/15 , D21C5/00 , D21H11/16 , D21H11/20 , D21H17/00 , D21H21/10
CPC分类号： C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C07K2319/00 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H17/005 , D21H21/10 , Y02P20/145 , Y02W30/43
摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) ThrArgXaaXaaAspCysCysXaaXaaXaaCysXaaTrpXaa 1234567891011121314 and a second amino acid sequence having the following sequence TrpCysCysXaaCys(SEQ ID NO: 80) 12345 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
摘要翻译：本发明涉及酶制剂，其基本上由具有纤维素分解活性的酶组成，并且包含具有以下序列的第一氨基酸序列和具有以下序列的第二个氨基酸序列，其中在第一序列的3位是氨基酸是Trp，Tyr或Phe; 在第一序列的位置4处，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 在第一序列的第9,10,12和14位，第2个序列的第4位，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列，（i）当第12位的氨基残基为Ser时，第14位的氨基酸残基不为Ser，（ii）当第12位的氨基残基为Gly时，第14位的氨基酸残基为 Ala在工业应用中表现良好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或衣服的磨损外观以及纸浆处理。此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。

9. 发明授权

US06855531B2 Endoglucanases 失效
标题翻译：内切葡聚糖酶
公开(公告)号：US06855531B2
公开(公告)日：2005-02-15
申请号：US10007521
申请日：2001-12-10
申请人： Torben Henriksen , Søren Flensted Lassen , Markus Sakari Kauppinen
发明人： Martin Shulein
IPC分类号： C12N15/09 , C05F11/08 , C05F17/00 , C07G99/00 , C11D3/386 , C12N1/19 , C12N9/42 , C12N15/00 , C12R1/645 , C12R1/69 , C12R1/77 , C12R1/80 , C12S3/04 , C12S11/00 , D06M13/02 , D06M13/322 , D06M13/46 , D06M16/00 , D06P5/02 , D06P5/15 , D21C5/00 , D21H11/16 , D21H11/20 , D21H17/00 , D21H21/10 , C07H21/04 , D05M16/00
CPC分类号： C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C07K2319/00 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H17/005 , D21H21/10 , Y02P20/145 , Y02W30/43
摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO:79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa 1 2 3 4 5 6 7 8 9 Xaa Cys Xaa Trp Xaa 10 11 12 13 14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys (SEQ ID NO:80) 1 2 3 4 5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
摘要翻译：本发明涉及酶制剂，其基本上由具有纤维素分解活性的酶组成，并且包含具有以下序列的第一氨基酸序列和具有以下序列的第二氨基酸序列，其中在第一序列的位置3，氨基酸为Trp ，Tyr或Phe; 在第一序列的位置4处，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 在第一序列的第9,10,12和14位，第2个序列的第4位，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列，（i）当第12位的氨基残基为Ser时，第14位的氨基酸残基不为Ser，（ii）当第12位的氨基残基为Gly时，第14位的氨基酸残基为 Ala在工业应用中表现非常好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或服装的磨损外观以及纸浆处理。此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。

10. 发明授权

US6156562A Strength loss resistant methods for improving the softening of cotton toweling and related fabrics 失效
标题翻译：用于改善棉毛巾及相关织物软化的强度损失方法
公开(公告)号：US6156562A
公开(公告)日：2000-12-05
申请号：US117648
申请日：1993-09-08
申请人： Thomas C. Cox
发明人： Thomas C. Cox
IPC分类号： C12N9/42 , C12R1/645 , C12S3/04 , D06B1/02 , D06M11/38 , D06M13/188 , D06M16/00 , D06M23/06 , D06M101/00 , D06M101/02 , D06M101/06
CPC分类号： C12N9/2437 , D06B1/02 , D06M11/38 , D06M13/188 , D06M16/003 , D06M23/06 , D06M2101/06 , D06M2200/50
摘要： Disclosed are strength loss resistant methods for treating cotton toweling with cellulase so as to impart permanent softening to the toweling. In particular, the methods disclosed herein involve the application of a specified amount of cellulase onto one or both of the surfaces of a cotton toweling so as to result in permanent softening of the toweling.
摘要翻译：公开了用纤维素酶处理棉毛巾的强度损失抗性方法，以便赋予毛巾永久软化。特别地，本文公开的方法涉及将特定量的纤维素酶施用于棉毛巾的一个或两个表面上，以便导致毛巾的永久软化。

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