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1. 发明申请

US20120052519A1 METHODS FOR ASSAYING ALPHA-L-IDURONIDASE ENZYMATIC ACTIVITY 审中-公开
标题翻译：用于测定ALPHA-L-IDURONIDASE酶活性的方法
公开(公告)号：US20120052519A1
公开(公告)日：2012-03-01
申请号：US13292371
申请日：2011-11-09
申请人： Michael H. Gelb , Sophie Blanchard
发明人： Michael H. Gelb , Sophie Blanchard
IPC分类号： C12Q1/34 , C07D311/18
CPC分类号： C12Q1/34 , C07D311/16 , C07D311/18 , C12Y302/01076 , G01N2333/924 , G01N2800/04 , G01N2800/042
摘要： Methods for assaying α-L-iduronidase enzymatic activity and methods for screening newborns for Mucopolysaccharidosis Type-I.
摘要翻译：用于测定α-L-真菌蛋白酶酶活性的方法和用于筛选I型粘多糖贮积病的新生儿的方法。

2. 发明授权

US06180114B2 Therapeutic delivery using compounds self-assembled into high axial ratio microstructures 失效
标题翻译：使用化合物自组装成高轴比微结构的治疗输送
公开(公告)号：US06180114B2
公开(公告)日：2001-01-30
申请号：US09219057
申请日：1998-12-22
申请人： Paul Yager , Michael H. Gelb , Anatoly N. Lukyanov , Alex S. Goldstein , Mary L. Disis
发明人： Paul Yager , Michael H. Gelb , Anatoly N. Lukyanov , Alex S. Goldstein , Mary L. Disis
IPC分类号： A61K900
CPC分类号： A61K48/00 , A61K9/1274 , A61K47/6919
摘要： Therapeutic complexes comprising plural therapeutic compounds self assembled into high axial ratio microstructures are described. The therapeutic complexes satisfy the formula HARM-Th, wherein HARM is a high axial ratio forming material and Th is a therapeutic coupled to or associated with the HARM. The therapeutic complexes also can satisfy the formula HARM-S-Th, wherein S is a spacer. Release of the therapeutic by the complex generally follows either 0-order kinetics or psuedo-first order kinetics. A method for delivering therapeutics to oransims, particularly humans, also is described. The method comprises administering an effective amount of (1) a ligand, such as a therapeutic, self-assembled into a HAR microstructure, or (2) a ligand, such as a therapeutic, coupled to or associated with a material capable of thereafter self-assembling into a high axial ratio microstructure, to the mammal. Nucleic acids are an example of a ligand that can be administered effectively according to this method through noncovalent attachment to the HARM-forming materials.
摘要翻译：描述了包含自组装成高轴比微结构的多种治疗化合物的治疗复合物。治疗复合物满足公式HARM-Th，其中HARM是高轴比形成材料，Th是与HARM偶联或与之相关的治疗剂。治疗复合物还可以满足式HARM-S-Th，其中S是间隔物。由复合物释放治疗剂通常遵循0级动力学或伪一级动力学。还描述了将治疗剂递送到人类，特别是人类的方法。该方法包括施用有效量的（1）配体，例如治疗性的，自组装的HAR微结构，或（2）配体，例如治疗剂，偶联或与其后自身的物质相关联 - 组装成高轴比比例的微结构，给哺乳动物。核酸是可以根据该方法通过非共价连接到形成HARM的材料而有效施用的配体的实例。

3. 发明授权

US09012663B2 Methods for assaying α-L-iduronidase enzymatic activity 有权
标题翻译：测定α-L-真菌蛋白酶酶活性的方法
公开(公告)号：US09012663B2
公开(公告)日：2015-04-21
申请号：US13292371
申请日：2011-11-09
申请人： Michael H. Gelb , Sophie Blanchard
发明人： Michael H. Gelb , Sophie Blanchard
IPC分类号： C07D311/16 , C07D311/18 , C12Q1/34
CPC分类号： C12Q1/34 , C07D311/16 , C07D311/18 , C12Y302/01076 , G01N2333/924 , G01N2800/04 , G01N2800/042
摘要： Methods for assaying α-L-iduronidase enzymatic activity and methods for screening newborns for Mucopolysaccharidosis Type-I.
摘要翻译：用于测定α-L-真菌蛋白酶酶活性的方法和用于筛选I型粘多糖贮积病的新生儿的方法。

4. 发明申请

US20110294153A1 REAGENT AND METHOD FOR DETECTION OF CARBOXYLIC ACIDS BY MASS SPECTROMETRY 有权
标题翻译：通过质谱法检测羧酸的试剂和方法
公开(公告)号：US20110294153A1
公开(公告)日：2011-12-01
申请号：US13078652
申请日：2011-04-01
申请人： Michael H. Gelb , Frantisek Turecek , James Bollinger
发明人： Michael H. Gelb , Frantisek Turecek , James Bollinger
IPC分类号： C12Q1/02 , G01N33/00 , C07D213/20
CPC分类号： C07D213/20 , G01N33/88 , G01N33/92 , H01J49/165 , Y10T436/174614
摘要： Method and reagent for converting a carboxylic acid to a positively charged amide. The method and reagent facilitate positive ion mass spectral analysis of carboxylic acids.
摘要翻译：将羧酸转化为带正电荷的酰胺的方法和试剂。该方法和试剂促进了羧酸的正离子质谱分析。

5. 发明授权

US06670194B1 Rapid quantitative analysis of proteins or protein function in complex mixtures 有权
标题翻译：蛋白质或蛋白质功能在复杂混合物中的快速定量分析
公开(公告)号：US06670194B1
公开(公告)日：2003-12-30
申请号：US09383062
申请日：1999-08-25
申请人： Rudolf Hans Aebersold , Michael H. Gelb , Steven P. Gygi , C. Ronald Scott , Frantisek Turecek , Scott A. Gerber , Beate Rist
发明人： Rudolf Hans Aebersold , Michael H. Gelb , Steven P. Gygi , C. Ronald Scott , Frantisek Turecek , Scott A. Gerber , Beate Rist
IPC分类号： C07C33116
CPC分类号： G01N33/6848 , C12Q1/25 , G01N33/58 , G01N33/6803 , G01N33/6842 , Y10S530/812 , Y10T436/182 , Y10T436/24 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e.g., by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e.g., products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amounts of proteins in different samples. The methods of this invention can be used for qualitative and quantitative analysis of global protein expression profiles in cells and tissues, to screen for and identify proteins whose expression level in cells, tissue or biological fluids is affected by a stimulus or by a change in condition or cell state of the cell, tissue or organism from which the sample originated.
摘要翻译：使用这些试剂的分析试剂和基于质谱的方法用于蛋白质混合物中蛋白质或蛋白质功能的快速和定量分析。该方法采用具有三个部分的亲和标记的蛋白质反应试剂：通过连接基团（L）与蛋白质反应基团（PRG）共价连接的亲和标记（A）。连接体可以是差异同位素标记的，例如用连接体中的一个或多个原子用其稳定同位素取代。这些试剂允许从复杂混合物中选择性分离肽片段或与给定蛋白质（例如，酶反应产物）反应的产物。分离的肽片段或反应产物是这些混合物中存在蛋白质或存在蛋白质功能的特征。分离的肽或反应产物的特征在于质谱（MS）技术。试剂还提供分离的肽或反应产物的差异同位素标记，其有助于通过质谱法定量测定不同样品中蛋白质的相对量。本发明的方法可用于细胞和组织中全球蛋白质表达谱的定性和定量分析，筛选和鉴定细胞，组织或生物流体中的表达水平受刺激或病情变化影响的蛋白质或来自样品的细胞，组织或生物体的细胞状态。

6. 发明申请

US20120122117A1 SECRETED PHOSPHOLIPASE A2 BIOMARKERS FOR ARTHRITIS 有权
标题翻译：分泌磷脂酶A2生物标志物
公开(公告)号：US20120122117A1
公开(公告)日：2012-05-17
申请号：US13145795
申请日：2010-01-22
申请人： David M. Lee , Eric Boilard , Michael H. Gelb , Reuben Gobezie
发明人： David M. Lee , Eric Boilard , Michael H. Gelb , Reuben Gobezie
IPC分类号： G01N33/573
CPC分类号： G01N33/573 , C12Q1/44 , G01N2333/92 , G01N2800/105 , G01N2800/52 , G01N2800/56
摘要： The present invention relates to the use of protein expression profiles of sPLA2 isoforms with clinical relevance to osteoarthritis (OA). In particular, the invention provides methods for diagnosing OA or determining risk factors for development of OA based on expression of sPLA2-IIA.
摘要翻译：本发明涉及与骨关节炎（OA）具有临床相关性的sPLA2同种型的蛋白质表达谱的用途。特别地，本发明提供了用于诊断OA或基于sPLA2-IIA表达确定OA发展的危险因素的方法。

7. 发明申请

US20100209951A1 METHODS FOR ASSAYING ALPHA-L-IDURONIDASE ENZYMATIC ACTIVITY 有权
标题翻译：用于测定ALPHA-L-IDURONIDASE酶活性的方法
公开(公告)号：US20100209951A1
公开(公告)日：2010-08-19
申请号：US12706794
申请日：2010-02-17
申请人： Michael H. Gelb , Sophie Blanchard
发明人： Michael H. Gelb , Sophie Blanchard
IPC分类号： C12Q1/34 , C07H15/207 , C07D311/18
CPC分类号： C12Q1/34 , C07D311/16 , C07D311/18 , C12Y302/01076 , G01N2333/924 , G01N2800/04 , G01N2800/042
摘要： Methods for assaying α-L-iduronidase enzymatic activity and methods for screening newborns for Mucopolysaccharidosis Type-I.
摘要翻译：用于测定α-L-真菌蛋白酶酶活性的方法和用于筛选I型粘多糖贮积病的新生儿的方法。

8. 发明授权

US07473535B2 Chemical reagents and methods for detection and quantification of proteins in complex mixtures 有权
标题翻译：用于复杂混合物中蛋白质的检测和定量的化学试剂和方法
公开(公告)号：US07473535B2
公开(公告)日：2009-01-06
申请号：US10225447
申请日：2002-08-20
申请人： Rudolf H. Aebersold , Patricia Q. Bottari , Michael H. Gelb , Frantisek Turecek
发明人： Rudolf H. Aebersold , Patricia Q. Bottari , Michael H. Gelb , Frantisek Turecek
IPC分类号： G01N33/53 , A61K38/00 , C07K1/00
CPC分类号： G01N33/585 , G01N33/6803 , G01N33/6809
摘要： The invention provides a reagent comprising an affinity tag, a detectable moiety, a linker, an isotope tag and a reactive group. The invention also provides methods of using a reagent of the invention. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions allowing the reactive group to bind to one or more polypeptides in the sample. The invention additionally provides methods of isolating, identifying and quantifying a polypeptide in a sample. The invention further provides methods of diagnosing a disease using a reagent of the invention.
摘要翻译：本发明提供了包含亲和标签，可检测部分，接头，同位素标签和反应性基团的试剂。本发明还提供了使用本发明试剂的方法。该方法可以用于在样品中通过使样品与本发明的试剂在允许反应性基团与样品中的一种或多种多肽结合的条件下接触来标记样品中的多肽。本发明另外提供了分离，鉴定和定量样品中的多肽的方法。本发明还提供使用本发明的试剂诊断疾病的方法。

9. 发明授权

US5066643A Fluorine and chlorine statine or statone containing peptides and method of use 失效
标题翻译：氟和氯他汀或含有肽的肽及其使用方法
公开(公告)号：US5066643A
公开(公告)日：1991-11-19
申请号：US466803
申请日：1990-01-18
申请人： Robert H. Abeles , Michael H. Gelb
发明人： Robert H. Abeles , Michael H. Gelb
IPC分类号： C07C271/22 , C07K5/02
CPC分类号： C07K5/0227 , C07C271/22
摘要： A peptide optionally in isosteric form wherein a methylene group in the backbone chain is disubstituted, one or both substituents being fluorine and/or chlorine, in free form or in pharmaceutically acceptable salt form, such as a compound of formula I ##STR1## wherein the substituents have various significances, or an isosteric form thereof,is useful as an enzyme inhibitor. In particular, as a renin inhibitor, it is useful in the prophylaxis or treatment of hypertension and congestive heart failure.It is prepared by a process comprising the step of coupling two corresponding peptide residues optionally in isosteric form, or precursors thereof, and if required appropriately converting any resultant compound in precursor form.
摘要翻译：任选处于等离子体形式的肽，其中主链中的亚甲基被二取代，游离形式或药学上可接受的盐形式的一种或两种取代基为氟和/或氯，例如式I化合物其中取代基具有各种含义，或其等排形式可用作酶抑制剂。特别是作为肾素抑制剂，可用于预防或治疗高血压和充血性心力衰竭。其通过包括以下步骤的方法制备，所述方法将两个相应的肽残基任选地以等排形式或其前体偶联，并且如果需要，则适当地转化任何得到的前体形式的化合物。

10. 发明授权

US08372594B2 Secreted phospholipase A2 biomarkers for arthritis 有权
标题翻译：分泌型磷脂酶A2生物标志物用于关节炎
公开(公告)号：US08372594B2
公开(公告)日：2013-02-12
申请号：US13145795
申请日：2010-01-22
申请人： David M. Lee , Eric Boilard , Michael H. Gelb , Reuben Gobezie
发明人： David M. Lee , Eric Boilard , Michael H. Gelb , Reuben Gobezie
IPC分类号： G01N33/53 , G01N31/00
CPC分类号： G01N33/573 , C12Q1/44 , G01N2333/92 , G01N2800/105 , G01N2800/52 , G01N2800/56
摘要： The present invention relates to the use of protein expression profiles of sPLA2 isoforms with clinical relevance to osteoarthritis (OA). In particular, the invention provides methods for diagnosing OA or determining risk factors for development of OA based on expression of sPLA2-IIA.
摘要翻译：本发明涉及与骨关节炎（OA）具有临床相关性的sPLA2同种型的蛋白质表达谱的用途。特别地，本发明提供了用于诊断OA或基于sPLA2-IIA表达确定OA发展的危险因素的方法。

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