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1. 发明申请

US20120122117A1 SECRETED PHOSPHOLIPASE A2 BIOMARKERS FOR ARTHRITIS 有权
标题翻译：分泌磷脂酶A2生物标志物
公开(公告)号：US20120122117A1
公开(公告)日：2012-05-17
申请号：US13145795
申请日：2010-01-22
申请人： David M. Lee , Eric Boilard , Michael H. Gelb , Reuben Gobezie
发明人： David M. Lee , Eric Boilard , Michael H. Gelb , Reuben Gobezie
IPC分类号： G01N33/573
CPC分类号： G01N33/573 , C12Q1/44 , G01N2333/92 , G01N2800/105 , G01N2800/52 , G01N2800/56
摘要： The present invention relates to the use of protein expression profiles of sPLA2 isoforms with clinical relevance to osteoarthritis (OA). In particular, the invention provides methods for diagnosing OA or determining risk factors for development of OA based on expression of sPLA2-IIA.
摘要翻译：本发明涉及与骨关节炎（OA）具有临床相关性的sPLA2同种型的蛋白质表达谱的用途。特别地，本发明提供了用于诊断OA或基于sPLA2-IIA表达确定OA发展的危险因素的方法。

2. 发明授权

US08372594B2 Secreted phospholipase A2 biomarkers for arthritis 有权
标题翻译：分泌型磷脂酶A2生物标志物用于关节炎
公开(公告)号：US08372594B2
公开(公告)日：2013-02-12
申请号：US13145795
申请日：2010-01-22
申请人： David M. Lee , Eric Boilard , Michael H. Gelb , Reuben Gobezie
发明人： David M. Lee , Eric Boilard , Michael H. Gelb , Reuben Gobezie
IPC分类号： G01N33/53 , G01N31/00
CPC分类号： G01N33/573 , C12Q1/44 , G01N2333/92 , G01N2800/105 , G01N2800/52 , G01N2800/56
摘要： The present invention relates to the use of protein expression profiles of sPLA2 isoforms with clinical relevance to osteoarthritis (OA). In particular, the invention provides methods for diagnosing OA or determining risk factors for development of OA based on expression of sPLA2-IIA.
摘要翻译：本发明涉及与骨关节炎（OA）具有临床相关性的sPLA2同种型的蛋白质表达谱的用途。特别地，本发明提供了用于诊断OA或基于sPLA2-IIA表达确定OA发展的危险因素的方法。

3. 发明授权

US09012663B2 Methods for assaying α-L-iduronidase enzymatic activity 有权
标题翻译：测定α-L-真菌蛋白酶酶活性的方法
公开(公告)号：US09012663B2
公开(公告)日：2015-04-21
申请号：US13292371
申请日：2011-11-09
申请人： Michael H. Gelb , Sophie Blanchard
发明人： Michael H. Gelb , Sophie Blanchard
IPC分类号： C07D311/16 , C07D311/18 , C12Q1/34
CPC分类号： C12Q1/34 , C07D311/16 , C07D311/18 , C12Y302/01076 , G01N2333/924 , G01N2800/04 , G01N2800/042
摘要： Methods for assaying α-L-iduronidase enzymatic activity and methods for screening newborns for Mucopolysaccharidosis Type-I.
摘要翻译：用于测定α-L-真菌蛋白酶酶活性的方法和用于筛选I型粘多糖贮积病的新生儿的方法。

4. 发明申请

US20110294153A1 REAGENT AND METHOD FOR DETECTION OF CARBOXYLIC ACIDS BY MASS SPECTROMETRY 有权
标题翻译：通过质谱法检测羧酸的试剂和方法
公开(公告)号：US20110294153A1
公开(公告)日：2011-12-01
申请号：US13078652
申请日：2011-04-01
申请人： Michael H. Gelb , Frantisek Turecek , James Bollinger
发明人： Michael H. Gelb , Frantisek Turecek , James Bollinger
IPC分类号： C12Q1/02 , G01N33/00 , C07D213/20
CPC分类号： C07D213/20 , G01N33/88 , G01N33/92 , H01J49/165 , Y10T436/174614
摘要： Method and reagent for converting a carboxylic acid to a positively charged amide. The method and reagent facilitate positive ion mass spectral analysis of carboxylic acids.
摘要翻译：将羧酸转化为带正电荷的酰胺的方法和试剂。该方法和试剂促进了羧酸的正离子质谱分析。

5. 发明授权

US06670194B1 Rapid quantitative analysis of proteins or protein function in complex mixtures 有权
标题翻译：蛋白质或蛋白质功能在复杂混合物中的快速定量分析
公开(公告)号：US06670194B1
公开(公告)日：2003-12-30
申请号：US09383062
申请日：1999-08-25
申请人： Rudolf Hans Aebersold , Michael H. Gelb , Steven P. Gygi , C. Ronald Scott , Frantisek Turecek , Scott A. Gerber , Beate Rist
发明人： Rudolf Hans Aebersold , Michael H. Gelb , Steven P. Gygi , C. Ronald Scott , Frantisek Turecek , Scott A. Gerber , Beate Rist
IPC分类号： C07C33116
CPC分类号： G01N33/6848 , C12Q1/25 , G01N33/58 , G01N33/6803 , G01N33/6842 , Y10S530/812 , Y10T436/182 , Y10T436/24 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e.g., by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e.g., products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amounts of proteins in different samples. The methods of this invention can be used for qualitative and quantitative analysis of global protein expression profiles in cells and tissues, to screen for and identify proteins whose expression level in cells, tissue or biological fluids is affected by a stimulus or by a change in condition or cell state of the cell, tissue or organism from which the sample originated.
摘要翻译：使用这些试剂的分析试剂和基于质谱的方法用于蛋白质混合物中蛋白质或蛋白质功能的快速和定量分析。该方法采用具有三个部分的亲和标记的蛋白质反应试剂：通过连接基团（L）与蛋白质反应基团（PRG）共价连接的亲和标记（A）。连接体可以是差异同位素标记的，例如用连接体中的一个或多个原子用其稳定同位素取代。这些试剂允许从复杂混合物中选择性分离肽片段或与给定蛋白质（例如，酶反应产物）反应的产物。分离的肽片段或反应产物是这些混合物中存在蛋白质或存在蛋白质功能的特征。分离的肽或反应产物的特征在于质谱（MS）技术。试剂还提供分离的肽或反应产物的差异同位素标记，其有助于通过质谱法定量测定不同样品中蛋白质的相对量。本发明的方法可用于细胞和组织中全球蛋白质表达谱的定性和定量分析，筛选和鉴定细胞，组织或生物流体中的表达水平受刺激或病情变化影响的蛋白质或来自样品的细胞，组织或生物体的细胞状态。

6. 发明授权

US09045421B2 Reagent and method for detection of carboxylic acids by mass spectrometry 有权
标题翻译：通过质谱法检测羧酸的试剂和方法
公开(公告)号：US09045421B2
公开(公告)日：2015-06-02
申请号：US13078652
申请日：2011-04-01
申请人： Michael H. Gelb , Frantisek Turecek , James Bollinger
发明人： Michael H. Gelb , Frantisek Turecek , James Bollinger
IPC分类号： C07D211/70 , C07D211/82 , C07D213/00 , C07D211/92 , C07D213/18 , C07D213/20 , G01N33/88 , G01N33/92 , H01J49/16
CPC分类号： C07D213/20 , G01N33/88 , G01N33/92 , H01J49/165 , Y10T436/174614
摘要： Method and reagent for converting a carboxylic acid to a positively charge amide are described. The method and reagent facilitate positive ion mass spectral analysis of carboxylic acids. The method includes reacting a carboxylic acid with a compound having formula I: wherein A and B are aromatic rings, ring A includes a quaternized nitrogen and has n additional ring atoms, each additional ring atom optionally substituted with an RA group, and n is an integer from 4 to 10, and ring B includes a carbon atom and has m additional ring atoms, each additional ring atom optionally substituted with an RB group, and m is an integer from 4 to 10. The compound includes at least one RA or RB group, and the at least one RA and RB group is -L-N(Z)H; and X− is a counterion.
摘要翻译：描述了将羧酸转化为正电荷酰胺的方法和试剂。该方法和试剂促进了羧酸的正离子质谱分析。该方法包括使羧酸与具有式I的化合物反应：其中A和B是芳环，环A包括季铵化的氮并具有n个另外的环原子，每个另外的环原子任选地被RA基团取代，n是 4〜10的整数，环B包含碳原子并具有另外的环原子，每个另外的环原子任选被一个RB基团取代，m是4-10的整数。该化合物包括至少一个RA或RB 并且所述至少一个RA和RB基团是-LN（Z）H; 而X-是抗衡离子。

7. 发明授权

US08802833B2 Method for 2-sulfation of glycosides 有权
标题翻译：糖苷的2-硫酸化方法
公开(公告)号：US08802833B2
公开(公告)日：2014-08-12
申请号：US13144048
申请日：2010-01-12
申请人： Michael H. Gelb , Sophie Blanchard
发明人： Michael H. Gelb , Sophie Blanchard
IPC分类号： C07H1/00 , C07H13/04 , C07H17/075
CPC分类号： C07H17/075 , C07H1/00 , C07H13/04
摘要： Method for selective 2-sulfation of glycosides.
摘要翻译：糖苷选择性2-硫酸化的方法。

8. 发明申请

US20110288281A1 METHOD FOR 2-SULFATION OF GLYCOSIDES 有权
标题翻译：甘油二硫化物的方法
公开(公告)号：US20110288281A1
公开(公告)日：2011-11-24
申请号：US13144048
申请日：2010-01-12
申请人： Michael H. Gelb , Sophie Blanchard
发明人： Michael H. Gelb , Sophie Blanchard
IPC分类号： C07H1/00
CPC分类号： C07H17/075 , C07H1/00 , C07H13/04
摘要： Method for selective 2-sulfation of glycosides.
摘要翻译：糖苷选择性2-硫酸化的方法。

9. 发明授权

US06852544B2 Rapid quantitative analysis of proteins or protein function in complex mixtures 有权
标题翻译：蛋白质或蛋白质功能在复杂混合物中的快速定量分析
公开(公告)号：US06852544B2
公开(公告)日：2005-02-08
申请号：US09839884
申请日：2001-04-20
申请人： Rudolf Hans Aebersold , Michael H. Gelb , Steven P. Gygi , C. Ronald Scott , Frantisek Turecek , Scott A. Gerber , Beate Rist
发明人： Rudolf Hans Aebersold , Michael H. Gelb , Steven P. Gygi , C. Ronald Scott , Frantisek Turecek , Scott A. Gerber , Beate Rist
IPC分类号： G01N27/62 , C12Q1/25 , C12Q1/34 , C12Q1/48 , G01N30/00 , G01N30/06 , G01N30/60 , G01N30/72 , G01N30/88 , G01N33/53 , G01N33/566 , G01N33/58 , G01N33/68
CPC分类号： G01N33/6848 , C12Q1/25 , G01N33/58 , G01N33/6803 , G01N33/6842 , Y10S530/812 , Y10T436/182 , Y10T436/24 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e.g., by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e.g., products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amounts of proteins in different samples. The methods of this invention can be used for qualitative and quantitative analysis of global protein expression profiles in cells and tissues, to screen for and identify proteins whose expression level in cells, tissue or biological fluids is affected by a stimulus or by a change in condition or state of the cell, tissue or organism from which the sample originated.
摘要翻译：使用这些试剂的分析试剂和基于质谱的方法用于蛋白质混合物中蛋白质或蛋白质功能的快速和定量分析。该方法采用具有三个部分的亲和标记的蛋白质反应试剂：通过连接基团（L）与蛋白质反应基团（PRG）共价连接的亲和标记（A）。连接体可以是差异同位素标记的，例如用连接体中的一个或多个原子用其稳定同位素取代。这些试剂允许从复杂混合物中选择性分离肽片段或与给定蛋白质（例如，酶反应产物）反应的产物。分离的肽片段或反应产物是这些混合物中存在蛋白质或存在蛋白质功能的特征。分离的肽或反应产物的特征在于质谱（MS）技术。试剂还提供分离的肽或反应产物的差异同位素标记，其有助于通过质谱法定量测定不同样品中蛋白质的相对量。本发明的方法可用于细胞和组织中全球蛋白质表达谱的定性和定量分析，筛选和鉴定细胞，组织或生物流体中的表达水平受刺激或病情变化影响的蛋白质或来自样品的细胞，组织或生物体的状态。

10. 发明申请

US20120052519A1 METHODS FOR ASSAYING ALPHA-L-IDURONIDASE ENZYMATIC ACTIVITY 审中-公开
标题翻译：用于测定ALPHA-L-IDURONIDASE酶活性的方法
公开(公告)号：US20120052519A1
公开(公告)日：2012-03-01
申请号：US13292371
申请日：2011-11-09
申请人： Michael H. Gelb , Sophie Blanchard
发明人： Michael H. Gelb , Sophie Blanchard
IPC分类号： C12Q1/34 , C07D311/18
CPC分类号： C12Q1/34 , C07D311/16 , C07D311/18 , C12Y302/01076 , G01N2333/924 , G01N2800/04 , G01N2800/042
摘要： Methods for assaying α-L-iduronidase enzymatic activity and methods for screening newborns for Mucopolysaccharidosis Type-I.
摘要翻译：用于测定α-L-真菌蛋白酶酶活性的方法和用于筛选I型粘多糖贮积病的新生儿的方法。

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