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1. 发明授权

US10421988B2 Method and assembly for determining cell vitalities 有权
公开(公告)号：US10421988B2
公开(公告)日：2019-09-24
申请号：US13499629
申请日：2010-09-29
申请人： Walter Gumbrecht , Markus Klöpzig , Peter Paulicka , Harald Schmidt , Manfred Stanzel
发明人： Walter Gumbrecht , Markus Klöpzig , Peter Paulicka , Harald Schmidt , Manfred Stanzel
IPC分类号： B01L3/00 , B01L1/00 , C12Q1/02 , C12Q1/18 , G01N33/50 , G01N35/00
摘要： The method includes binding living cells to magnetic particles, adding them to a sensor array, uniformly distributing over the sensor array, magnetically fixing the magnetic particles having the bound cells over the sensor array, and adding substances to maintain and/or improve the cell vitality to the sensory array, and/or adding substances to worsen the cell vitality to the sensor array. The assembly includes a sensor array composed of sensors, which are designed to be in direct fluidic contact with a fluid, and a device for generating a magnetic field over the sensor array. A layer that comprises magnetic particles and living cells is formed on the sensor array.

2. 发明授权

US09958435B2 Arrangement and process for optical analysis and specific isolation of biological samples 有权
公开(公告)号：US09958435B2
公开(公告)日：2018-05-01
申请号：US14118723
申请日：2012-04-16
申请人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
发明人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
IPC分类号： G01N33/50 , G01N1/28 , B01D63/08 , B01L3/00 , G01N15/10 , G01N1/04 , G01N1/40
CPC分类号： G01N33/5091 , B01D63/08 , B01L3/508 , B01L2300/0681 , B01L2300/0822 , G01N1/2813 , G01N2001/045 , G01N2001/4088 , G01N2015/1006
摘要： A process is disclosed for detecting cells in a liquid sample, which includes: i) filtration of the liquid sample through a porous membrane which is suitable for retaining detectable cells, where at least one subregion of a support is configured as transparent supporting body and the membrane is arranged over its area on the transparent supporting body in such a way that detectable cells are retained on at least part of the surface of the membrane and that at least part of the sample liquid passes through the membrane, ii) application of a liquid optical medium which has essentially the same refractive index as the supporting body, and iii) optical measurement of at least a subarea of the membrane in order to detect detectable cells.

3. 发明授权

US09267616B2 Method for controlling valves during the thermocyclization of a substance for the purpose of polymer chain reaction (PCR) and associated arrangement 有权
标题翻译：为了聚合物链反应（PCR）和相关布置，在物质热循环期间控制阀的方法
公开(公告)号：US09267616B2
公开(公告)日：2016-02-23
申请号：US11665297
申请日：2005-10-14
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： C12M1/00 , F16K99/00 , B01L3/00 , B01L7/00
CPC分类号： F16K99/0001 , B01L3/502738 , B01L7/52 , B01L2300/0877 , B01L2300/1822 , B01L2400/0633 , B01L2400/0661 , C08L2201/12 , F16K99/0036 , F16K99/0038 , F16K99/0044 , F16K2099/0084
摘要： At least one embodiment of the invention relates to an arrangement for thermocyclization of a substance. At least one embodiment of the invention also relates to a method for the thermocyclization of a substance, including: the controllable valves automatically close the PCR chamber after the test fluid has been introduced into the PCR-chamber, and the properties for the memory metal or bimetal elements are used for closing the valves when a predetermined temperature has been exceeded. As a result, the mechanical actuator, which is used to actuate the valves, is thermally coupled to the heating/cooling element in order to carry out the thermocyclization.
摘要翻译：本发明的至少一个实施方案涉及物质热循环的装置。本发明的至少一个实施方案还涉及一种用于物质热循环的方法，包括：在将测试流体引入PCR室之后，可控阀自动关闭PCR室，以及存储金属或当超过预定温度时，双金属元件用于关闭阀。因此，用于致动阀的机械致动器与加热/冷却元件热耦合以便进行热循环。

4. 发明申请

US20140106388A1 AUTOMATED CTC DETECTION 审中-公开
标题翻译：自动CTC检测
公开(公告)号：US20140106388A1
公开(公告)日：2014-04-17
申请号：US14118701
申请日：2012-04-13
申请人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
发明人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
IPC分类号： G01N33/50
CPC分类号： G01N33/5091 , C12Q1/6806 , G01N1/30
摘要： An embodiment relates to a method and to an array for detecting live circulating or disseminated cells in body fluids (for example blood, urine) or tissue samples (for example bone marrow) mixed with liquid. The method includes: a) filtering the liquid sample through a porous membrane that is suitable for retaining cells to be detected, such that cells to be detected come to rest upon at least a part of the surface of the membrane and the sample liquid passes the membrane; b) applying a first process liquid containing a first agent for marking the cells to be detected with a first marker; c) incubating the process liquid on the membrane for a predetermined time period, wherein the cells to be detected are marked; and d) detecting the marked cells to be detected on the surface of the membrane.
摘要翻译：实施方案涉及用于检测与液体混合的体液（例如血液，尿液）或组织样品（例如骨髓）中的活的循环或播散细胞的方法和阵列。该方法包括：a）通过适于保留待检测细胞的多孔膜过滤液体样品，使待检测细胞停留在膜表面的至少一部分上，样品液体通过膜; b）施加含有用于用第一标记标记待检测细胞的第一试剂的第一处理液; c）将所述工艺液体在所述膜上孵育预定时间段，其中所述待检测的细胞被标记; 以及d）检测在膜的表面上待检测的标记细胞。

5. 发明授权

US08551737B2 Method for analysing amplified nucleic acids 有权
标题翻译：分析扩增核酸的方法
公开(公告)号：US08551737B2
公开(公告)日：2013-10-08
申请号：US12308552
申请日：2007-05-31
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6827 , B01L3/5027 , C12Q1/6846 , C12Q2565/629 , C12Q2527/125
摘要： An example embodiment of the present invention discloses a method for analyzing nucleic acids in a microfluidic device. The method includes the following steps: a) nucleic acids are amplified in a first chamber in the microfluidic device; b) the amplified nucleic acids are brought into contact with an additive including: i) monovalent cations and ii) an Mg2+ ion-binding agent, the additive being provided in a second chamber in the microfluidic device; and c) the amplified nucleic acids are hybridized on at least one probe oligonucleotide.
摘要翻译：本发明的示例性实施方案公开了一种用于分析微流体装置中的核酸的方法。该方法包括以下步骤：a）核酸在微流体装置的第一腔室中扩增; b）使扩增的核酸与添加剂接触，包括：i）一价阳离子和ii）Mg 2+离子结合剂，所述添加剂设置在微流体装置中的第二室中; 和c）扩增的核酸在至少一种探针寡核苷酸上杂交。

6. 发明申请

US20120090692A1 VALVE FOR LAB-ON-A-CHIP SYSTEMS, METHOD FOR ACTUATING AND FOR PRODUCING VALVE 有权
标题翻译：用于制动阀门的阀门，用于制动阀门的方法
公开(公告)号：US20120090692A1
公开(公告)日：2012-04-19
申请号：US13375200
申请日：2010-04-29
申请人： Katja Friedrich , Walter Gumbrecht , Peter Paulicka
发明人： Katja Friedrich , Walter Gumbrecht , Peter Paulicka
IPC分类号： F15D1/00 , B29C45/16 , B32B37/16 , B01L99/00
CPC分类号： F16K99/0001 , B01L3/5027 , B01L2200/10 , B01L2300/0816 , B01L2300/0887 , B01L2400/0655 , F16K7/20 , F16K99/0026 , F16K2099/0078 , F16K2099/0084 , Y10T137/0396
摘要： A substrate of a lab-on-a-chip system has two adjacent recesses, one serving as a flow channel and the other one being filled with an elastomer compound. In a first state, the elastomer compound and the substrate delimit the flow channel in a section. In a second state, the elastomer compound takes up the space in the recess in the substrate along a cross-section of the flow channel, thereby completely closing the flow channel. The substrate and the elastomer compound may be produced by injection molding techniques.
摘要翻译：实验室芯片系统的基板具有两个相邻的凹槽，一个用作流动通道，另一个用弹性体化合物填充。在第一状态下，弹性体化合物和基底在一段中限定流动通道。在第二状态下，弹性体化合物沿着流路的横截面占据基板的凹部的空间，从而完全封闭流路。基材和弹性体化合物可以通过注塑技术制备。

7. 发明申请

US20110313257A1 Method for Recording and Evaluation of Measurements Related to the Condition of a Patient for Configuration of a Portable, Patient-Controlled Device Operated to Record Said Measurements and Related Device 审中-公开
标题翻译：用于记录和评估与用于记录所述测量和相关设备的便携式患者控制设备的配置的病人状况相关的测量的方法
公开(公告)号：US20110313257A1
公开(公告)日：2011-12-22
申请号：US13140908
申请日：2009-11-27
申请人： Klaus Abraham-Fuchs , Maximilian Fleischer , Karsten Hiltawsky , Oliver Hornung , Thomas Krüger-Sundhaus , Peter Paulicka , Roland Pohle , Oliver Von Sicard
发明人： Klaus Abraham-Fuchs , Maximilian Fleischer , Karsten Hiltawsky , Oliver Hornung , Thomas Krüger-Sundhaus , Peter Paulicka , Roland Pohle , Oliver Von Sicard
IPC分类号： A61B5/00
CPC分类号： A61B5/08 , A61B5/0002 , A61B5/082 , A61B5/7264 , A61B2560/0271 , G06F19/3418 , G16H40/40 , G16H50/20
摘要： In a method for recording and evaluating measurements related to the condition of a patient for configuration of a portable, patient-controlled device operated to record the measurements,—at least one measurement describing the condition of the patient is recorded by the device,—the measurement and/or at least one parameter derived therefrom is/are transferred to an evaluation device via a communication connection,—the measurement and/or the parameter is/are evaluated by or on the evaluation device by an expert system and/or an expert to determine patient-specific information,—configuration data ascertained in consideration of the patient-specific information is transferred back to the device via the or another communication connection, and—the device is configured according to the configuration data.
摘要翻译：在用于记录和评估与用于配置用于记录测量的操作的便携式，患者控制的装置的患者的状况相关的测量的测量方法中，至少一个描述病人状况的测量由设备记录，-the 测量和/或从其导出的至少一个参数经由通信连接传送到评估装置，测量和/或参数由评估装置由专家系统和/或专家评估或评估为了确定患者特定信息，考虑到患者特定信息确定的构造数据经由或另一通信连接被传送回设备，并且根据配置数据配置设备。

8. 发明授权

US07993828B2 PCR process and arrangement for DNA amplification using dry reagents 有权
标题翻译：使用干试剂进行DNA扩增的PCR过程和排列
公开(公告)号：US07993828B2
公开(公告)日：2011-08-09
申请号：US11587567
申请日：2005-04-26
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686
摘要： A PCR process for DNA amplification with thermocyclisation of the corresponding reagents is disclosed, in which total integration of all substances and process steps is achieved in a closed, single-use unit (so-called cartridge) in which the reagents are stored in a storage-stable form at room temperature. According to the process, the water-soluble reagents are covered by a water-insoluble medium, then the DNA to be amplified is supplied and the water-insoluble medium is eliminated, so that the water-soluble reagents are dissolved and PCR can start. In the corresponding arrangement, a test unit designed as a single-use produce (a so-called cartridge) has at least one micro-channel or micro-cavity for receiving a PCR reagent. The PCR reagents in the form of a mixture which can be dried at a negligible vapour pressure and forms a storage-stable substance substance at room temperature adhere to the walls of the micro-channel or micro-cavity and form a thin film covered by an insoluble medium.
摘要翻译：公开了用于相应试剂的热循环的DNA扩增的PCR方法，其中所有物质和方法步骤的完全整合在封闭的一次性使用单元（所谓的盒）中实现，其中试剂存储在储存器 - 稳定形式在室温下。根据该方法，水溶性试剂被水不溶性介质覆盖，然后供给待扩增的DNA，除去水不溶性介质，溶解水溶性试剂并开始PCR。在相应的布置中，设计为单次使用的产品（所谓的盒）的测试单元具有用于接收PCR试剂的至少一个微通道或微腔。可以以可忽略的蒸气压干燥并在室温下形成储存稳定物质物质的混合物形式的PCR试剂粘附到微通道或微腔的壁上并形成由不溶性介质。

9. 发明申请

US20110076690A1 TITER PLATE, READING DEVICE THEREFOR AND METHOD FOR DETECTING AN ANALYTE, AND USE THEREOF 有权
标题翻译： TITER板，其读取装置和检测分析仪的方法及其使用
公开(公告)号：US20110076690A1
公开(公告)日：2011-03-31
申请号：US12994906
申请日：2009-05-22
申请人： Walter Gumbrecht , Peter Paulicka
发明人： Walter Gumbrecht , Peter Paulicka
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L3/5025 , B01L7/52 , B01L2200/025 , B01L2200/0689 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2300/069 , B01L2300/0829
摘要： A titer plate and a method for detecting an analyte, and the use thereof are disclosed. According to at least one embodiment of the invention, it is proposed that a plurality of depressions and a biochip of the titer plate sposed adjacent thereto be surrounded by a wall in order to effectively prevent sample contamination when there is a high degree of spatial integration.
摘要翻译：公开了滴定板和检测分析物的方法及其用途。根据本发明的至少一个实施方案，提出了与壁相邻的滴定板的多个凹陷和生物芯片被壁包围，以便当存在高度的空间整合时有效地防止样品污染。

10. 发明申请

US20110021363A1 APPARATUS AND METHOD COMPRISING A SENSOR ARRAY AND A POROUS PLUNGER AND USE THEREOF 有权
标题翻译：装置和方法，包括一个传感器阵列和一个多孔的冲洗器及其使用
公开(公告)号：US20110021363A1
公开(公告)日：2011-01-27
申请号：US12867561
申请日：2009-02-09
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： C40B30/00 , G01N33/00 , C40B60/00
CPC分类号： G01N33/5438 , B01J2219/00382 , B01J2219/00527 , B01J2219/00704 , B01L3/502707 , B01L3/502715 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2300/087 , B01L2400/0421 , B01L2400/0427 , G01N27/27 , G01N27/3277
摘要： Disclosed is an apparatus that is used for detecting liquids or substances from liquids and includes a plunger that has a porous plunger layer which is pressed onto a sensor array. Each sensor of the sensor array is surrounded by elevations which spatially separate the sensors from each other like walls. In at least one embodiment, when the plunger layer is pressed onto the sensor array, the walls are pressed into the pores of the plunger layer. Liquid-tight connections are created between the walls and the plunger layer while liquid remains over the sensors. The liquid can be measured. In at least one embodiment, when there is direct mechanical contact between the plunger layer and the sensors, the liquid over the sensors is located in open pores on the surface of the plunger layer. No liquid flows between the pores and across the walls when pressure is applied to the plunger layer such that closed reaction chambers are created.
摘要翻译：公开了一种用于从液体中检测液体或物质的装置，包括具有压在传感器阵列上的多孔柱塞层的柱塞。传感器阵列的每个传感器被高度包围，空间上将传感器彼此像墙壁分开。在至少一个实施例中，当柱塞层被压到传感器阵列上时，壁压入柱塞层的孔中。在壁和柱塞层之间产生液密连接，而液体保留在传感器上方。可以测量液体。在至少一个实施例中，当柱塞层和传感器之间存在直接机械接触时，传感器上的液体位于柱塞层表面上的开放孔中。当压力施加到柱塞层时，没有液体流过孔隙和壁之间，从而形成封闭的反应室。

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