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    • 5. 发明申请
    • System For Purifying, Producing And Storing Biomolecules
    • 净化,生产和储存生物分子系统
    • US20140037961A1
    • 2014-02-06
    • US14111305
    • 2012-04-12
    • David Evans
    • David Evans
    • C07C319/28C07K17/02C07C323/58C07K1/22C07C59/347
    • C07C319/28A61K47/6817A61K47/6855B01J20/287B01J20/3212B01J20/3219B01J20/3248B01J20/3251B01J20/3274C07C59/347C07C323/58C07K1/22C07K17/02Y10T428/2982
    • The invention relates to a lock-release method to be applied to biomolecules, such as antibodies, to improve the purification, production, stability and storage of biomolecules. A biomolecule is covalently bound to a polymer support comprising a diketone group so that the biomolecule can be purified, produced and/or stored before being released from the support. The diketone group of the polymer support is a 1,3-ketoester, 1,3-ketothioester or 1,3-ketoamide is a group of Formula (1): R1 is an optionally substituted hydrocarbyl, perhalogenated hydrocarbyl, or a heterocyclyl group; Y is hydrogen, an optionally substituted hydrocarbyl, or a heterocyclyl group; X is —O, —NR2 or —S, wherein the free valence of —O, —NR2 or —S is bonded to the support optionally via a linker; and R2 is hydrogen, an optionally substituted hydrocarbyl, or a heterocyclyl group. The invention also relates to a polymer support comprising the diketone group.
    • 本发明涉及一种应用于生物分子如抗体的锁定释放方法,以改善生物分子的纯化,生产,稳定性和储存。 生物分子与包含二酮基团的聚合物载体共价结合,使得生物分子可以在从载体释放之前被纯化,产生和/或储存。 聚合物载体的二酮基团是1,3-酮酯,1,3-酮硫代酯或1,3-酮酰胺是式(1)的基团:R 1是任选取代的烃基,全卤代烃基或杂环基; Y是氢,任选取代的烃基或杂环基; X是-O,-NR 2或-S,其中-O,-NR 2或-S的游离基任选地经由连接基与支持基结合; 和R 2是氢,任选取代的烃基或杂环基。 本发明还涉及包含二酮基团的聚合物载体。