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    • 9. 发明申请
    • CELL MEASURING VESSEL, EXTRACELLULAR POTENTIAL MEASURING METHOD, AND CHEMICAL TESTING METHOD
    • 细胞测量容器,细胞电位测量方法和化学测试方法
    • US20100304423A1
    • 2010-12-02
    • US12733729
    • 2008-09-17
    • Yasuyuki AsaiNagakura MakotoFukami TakeakiOosumi Keisuke
    • Yasuyuki AsaiNagakura MakotoFukami TakeakiOosumi Keisuke
    • C12Q1/02C12M1/34
    • B01L3/5085B01L2300/0645B01L2300/0829C12M41/36G01N33/4836G01N33/48728
    • The cell observation using a conventional well plate takes much costs. Each well 3 being opened at a top plate 2a of the well plate 1 has a diameter reduced portion 32 which inner circumferential surface 32a is conically hollowed. At a lower portion of the diameter reduced portion 32 is formed an inserting hole 33. In a detecting portion 4, an outer circumferential surface of a round-bar reference electrode 41 is covered by an insulating portion 42. The detecting portion 4 has an upper end portion 4a and a lower end portion 4b which outer circumferential surface is exposed to an outside without being covered by an insulating portion 42. On an upper side above the lower end portion 4b is formed a diameter expanded portion 4c in which an outer circumferential surface of the insulating portion 42 is covered with a measuring electrode 43. The detecting portion 4 is fixed to a well 3 whose diameter expanded portion 4c is inserted into an inserting hole 33 and its upper end portion 4a is contained in the well 3. In the detecting portion 4, the upper end surface 43a of the measuring electrode 43 is communicated with an inner circumferential surface 32a of the diameter reduced portion 32 positioned in an edge surrounding portion of the inserting hole 33.
    • 使用常规孔板的细胞观察需要很多成本。 在井板1的顶板2a上开口的各孔3具有直径减小部32,该内周面32a是锥形中空的。 在直径减小部32的下部形成有插入孔33.在检测部4中,圆棒状基准电极41的外周面被绝缘部42覆盖。检测部4具有上部 端部4a和下端部4b,该外周面暴露于外部而不被绝缘部42覆盖。在下端部4b上方的上侧形成有直径扩大部4c,其中外周面 绝缘部分42被测量电极43覆盖。检测部分4固定到孔3,孔3的直径扩大部分4c插入插入孔33中,并且其上端部分4a包含在井3中。在 检测部分4,测量电极43的上端表面43a与位于边缘周边的直径减小部分32的内周表面32a相连通 g部分插入孔33。