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    • 7. 发明授权
    • Spin column system and methods
    • 自旋柱系统和方法
    • US08377394B2
    • 2013-02-19
    • US12835886
    • 2010-07-14
    • Robert SakowskiScott Fulton
    • Robert SakowskiScott Fulton
    • B01D45/00
    • G01N35/1074B01L3/50255B01L7/00B01L9/543B01L2300/0829B01L2400/0409G01N30/467G01N30/6043G01N30/6091G01N2035/1053Y10T436/255
    • The present invention provides a low bed-volume spin column system and methods of use. The system includes various combinations of spin columns with packed beds, a rack for holding the spin columns, a receiver plate that attaches to the rack and that has wells in registration with the spin columns held in the rack, a lid that attaches to the receiver plate and seals the spin columns, and an incubator block configured for accepting the wells of the receiver plate and for incubating the packed bed. The lid, spin columns, rack, receiver plate, and incubator block are preferably capable of being assembled in a nested configuration. In preferred versions of the invention, the rack is compatible with and attachable to conventional 96-well microplates. Further provided are methods of using the spin column system that include quantitatively purifying and analyzing an analyte, removing and preventing air entrainment within the packed bed, and incubating the packed bed while preventing drying thereof.
    • 本发明提供了低床体积旋转塔系统和使用方法。 该系统包括旋转柱与填充床的各种组合,用于保持旋转柱的支架,附接到机架的接收板,并且具有与保持在机架中的旋转柱对准的孔的接收器板,附接到接收器的盖 板和密封旋转柱,以及配置用于接收接收器板的孔并用于孵育填充床的培养箱。 盖,旋转柱,齿条,接收板和培养箱块优选能够以嵌套构型组装。 在本发明的优选形式中,机架与传统的96孔微孔板兼容并可附接。 还提供了使用旋转柱系统的方法,其包括定量纯化和分析分析物,去除和防止填充床内的空气夹带,并且在填充床的同时防止其干燥。
    • 10. 发明授权
    • Channeled polymer fibers as stationary/support phases for chemical separation by liquid chromatography and for waste stream clean-up
    • 引导聚合物纤维作为固定/支持阶段,用于通过液相色谱进行化学分离和废物流净化
    • US07374673B2
    • 2008-05-20
    • US10485701
    • 2002-08-13
    • R. Kenneth Marcus
    • R. Kenneth Marcus
    • B01D15/08
    • B01J20/28023B01J20/28014B01J20/281B01J20/285B01J2220/54G01N30/48G01N30/52G01N30/60G01N30/6043Y10T436/25375G01N30/34G01N30/466
    • Polymer fibers having a novel cross-sectional geometry are used as stationary phase materials for liquid chromatography separations. Fibers of 20 to 50 micrometer diameters have surface-channel structures extending their entire lengths. Bundles of fibers having this novel cross-sectional geometry are packed in columns. Different polymer compositions permit the “chemical tuning” of the separation process. Channeled fibers composed of polystyrene and polypropylene have been used to separate mixtures of polyaromatic hydrocarbons (PAHs), Pb-containing compounds and fatty acids. Use of channeled fibers allows a wide range of liquid flow rates with very low backing pressures. Applications in HPLC, cap-LC, prep-scale separations, analytical separations, single fiber separations, waste remediation/immobilization, extraction of selected organic molecules/ions from solution, purification of liquid streams (process waste, drinking water, pure solvents), selective extraction of cell matter and bacteria from growth media, and immobilization of cell matter and bacteria are envisioned.
    • 具有新颖横截面几何形状的聚合物纤维用作液相色谱分离的固定相材料。 20至50微米直径的纤维具有延伸其整个长度的表面通道结构。 具有这种新颖的横截面几何形状的纤维束被包装在柱中。 不同的聚合物组合物允许分离过程的“化学调节”。 已经使用由聚苯乙烯和聚丙烯组成的通道纤维来分离多芳烃(PAH),含Pb化合物和脂肪酸的混合物。 通道纤维的使用允许具有非常低的背压的宽范围的液体流速。 在HPLC,cap-LC,预分离分离,分析分离,单纤维分离,废物修复/固定,从溶液中萃取选定的有机分子/离子,液体流净化(工艺废物,饮用水,纯溶剂), 从生长培养基中选择性提取细胞和细菌,并设想细胞和细菌的固定。