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    • 4. 发明授权
    • Sequencing using a predetermined coverage amount of polynucleotide fragments
    • 使用预定覆盖量的多核苷酸片段进行测序
    • US08771957B2
    • 2014-07-08
    • US13975215
    • 2013-08-23
    • Callida Genomics, Inc.
    • Radoje Drmanac
    • C12Q1/68
    • C12Q1/6874C07H21/04C07K1/047C12Q1/6806C12Q1/682C12Q1/6837C12Q1/6869C12Q2525/151C12Q2525/313C12Q2531/125C12Q2565/513G01N15/1404G01N15/1434Y10S977/778Y10S977/789Y10S977/792Y10S977/88Y10S977/882C12Q2521/307C12Q2525/161C12Q2535/122C12Q2563/179C12Q2565/514
    • The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.
    • 本发明提供了用于排序从一个或多个目标多核苷酸衍生的序列信息的方法和试剂盒。 在一个方面,产生一个或多个分层或等级的碎片和等分试样,之后从最终级别或层级的片段获得序列信息。 这样的最后一层中的每个片段都来自特定的等分试样,而这些等分试样又是来自先前层的特定等分试样,等等。 对于最后一层中的等分试样的每个片段,从每个先前的层次派生的等分试样是已知的,或者可以被辨别出来。 因此,来自不同等分试样的重叠片段的相同序列可以被区分并分组为从与先前层相同或不同的片段衍生的。 当最终层中的片段被排序时,使用不同等分试样的片段的重叠序列区域来登记片段,使得非重叠区域被排序。 在一个方面,该方法以分级方式进行,直到一个或多个目标多核苷酸被表征为例如。 通过其核酸序列,或通过序列片段的排序,或通过单核苷酸多态性(SNP)等的排序。
    • 5. 发明申请
    • METHOD FOR PREPARING GRAPHENE BY USING TWO-DIMENSIONAL CONFINED SPACE BETWEEN THE LAYERS OF INORGANIC LAYERED MATERIALS
    • 通过在无机层材料层之间使用两维限定空间来制备石墨的方法
    • US20140154165A1
    • 2014-06-05
    • US13980715
    • 2012-03-27
    • Wensheng YangJie SunHaimei LiuXu ChenXue Duan
    • Wensheng YangJie SunHaimei LiuXu ChenXue Duan
    • C01B31/04
    • C01B31/0446C01B32/192C01B32/22Y10S977/789
    • The present invention relates to a method for preparing graphene using the two-dimensional confined space between the layers of inorganic layered materials. Such method comprises the following steps: mix a soluble salt of a divalent metal ion M2+, a soluble salt of a trivalent metal ion M′3+, a soluble salt of a chain alkyl anion A− and a carbon source molecule C and dissolve them in deionized and CO2-eliminated water to prepare a mixed salt solution; mix the mixed salt solution with an alkali solution under nitrogen protection and subject them to reaction and crystallization under nitrogen, and filter the suspension obtained thereafter and wash the filter cake with deionized water until the pH of the filtrate is 7 to 7.5, and then dry the filter cake to obtain layered double hydroxides with an intercalated structure; under an inert atmosphere or a reducing atmosphere, calcinate the layered double hydroxides with an intercalated structure to provide a calcinated product; add the calcinated product into a hydrochloric acid solution for ultrasonic treatment, and separate the solution by centrifugation and wash the precipitate obtained by centrifugation with deionized water until the pH of the filtrate is 6.5 to 7 to obtain the graphene.
    • 本发明涉及使用无机层状材料层之间的二维密闭空间来制备石墨烯的方法。 该方法包括以下步骤:将二价金属离子M2 +的可溶性盐,三价金属离子M'3 +的可溶性盐,链烷基阴离子A的可溶性盐和碳源分子C混合并溶解 在去离子水和二氧化碳消除的水中制备混合盐溶液; 在氮气保护下将混合盐溶液与碱溶液混合,并在氮气下进行反应和结晶,过滤后得到的悬浮液,用去离子水洗涤滤饼直到滤液的pH为7至7.5,然后干燥 滤饼得到具有插层结构的层状双氢氧化物; 在惰性气氛或还原气氛下,以层间结构煅烧层状双氢氧化物,得到煅烧产物; 将煅烧后的产物加入到盐酸溶液中进行超声波处理,离心分离溶液,用去离子水离心洗涤沉淀,直到滤液的pH值达到6.5〜7,得到石墨烯。
    • 6. 发明授权
    • Using non-overlapping fragments for nucleic acid sequencing
    • 使用非重叠片段进行核酸测序
    • US08673562B2
    • 2014-03-18
    • US13954778
    • 2013-07-30
    • Callida Genomics, Inc.
    • Radoje Drmanac
    • C12Q1/68
    • C12Q1/6874C07H21/04C07K1/047C12Q1/6806C12Q1/682C12Q1/6837C12Q1/6869C12Q2525/151C12Q2525/313C12Q2531/125C12Q2565/513G01N15/1404G01N15/1434Y10S977/778Y10S977/789Y10S977/792Y10S977/88Y10S977/882C12Q2521/307C12Q2525/161C12Q2535/122C12Q2563/179C12Q2565/514
    • The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.
    • 本发明提供了用于排序从一个或多个目标多核苷酸衍生的序列信息的方法和试剂盒。 在一个方面,产生一个或多个分层或等级的碎片和等分试样,之后从最终级别或层级的片段获得序列信息。 这样的最后一层中的每个片段都来自特定的等分试样,而这些等分试样又是来自先前层的特定等分试样,等等。 对于最后一层中的等分试样的每个片段,从每个先前的层次派生的等分试样是已知的,或者可以被辨别出来。 因此,来自不同等分试样的重叠片段的相同序列可以被区分并分组为从与先前层相同或不同的片段衍生的。 当最终层中的片段被排序时,使用不同等分试样的片段的重叠序列区域来登记片段,使得非重叠区域被排序。 在一个方面,该方法以分级方式进行,直到一个或多个目标多核苷酸被表征为例如。 通过其核酸序列,或通过序列片段的排序,或通过单核苷酸多态性(SNP)等的排序。