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    • 9. 发明授权
    • Method for nucleic acids isolation
    • 核酸分离方法
    • US08809519B2
    • 2014-08-19
    • US12747802
    • 2008-12-12
    • Jesus Ching
    • Jesus Ching
    • C07H21/00C07C315/00C07C317/00
    • B01J20/3251B01D15/424B01J20/286C12N15/1006
    • Methods for isolating nucleic acids using a solid phase having alkylene sulfonyl-containing compounds on its surface are provided. In one embodiment, the method comprises: contacting a sample containing nucleic acids with a solid phase having alkylene sulfonyl-containing compounds on its surface in a first aqueous solution to provide a loaded solid phase; washing the loaded solid phase with a second aqueous solution to provide a washed solid phase; and eluting the washed solid phase with a low ionic strength liquid to obtain the isolated nucleic acids. Kits containing a solid phase having alkylene sulfonyl-containing compounds also are provided.
    • 提供了使用其表面上含亚烷基磺酰基化合物的固相分离核酸的方法。 在一个实施方案中,所述方法包括:使含有核酸的样品与其表面上含有亚烷基磺酰基的化合物在第一水溶液中接触以提供负载的固相; 用第二水溶液洗涤负载的固相以提供洗涤的固相; 并用低离子强度液体洗脱洗涤的固相以获得分离的核酸。 也提供了含有含亚烷基磺酰基化合物的固相的试剂盒。